Stam2 (NM_019667) Mouse Tagged ORF Clone

CAT#: MG208368

Stam2 (tGFP-tagged) - Mouse signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (Stam2)

Product Images

Circular map for MG208368

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Stam2
• Synonyms: 1200004O12Rik; 5730456G07Rik; BB145015; Hbp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208368 representing NM_019667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTGTTCACTGCCAACCCGTTCGAGCAAGATGTGGAAAAAGCCACAAATGAGTACAACACCACAG
  AAGACTGGAGTCTGATCATGGACATCTGTGACAGGGTTGGAAGCACTCCCAGTGGAGCAAAAGATTGCCT
  AAAAGCCATAATGAAAAGGGTAAACCATAAGGTCCCTCACGTTGCTCTGCAGGCATTGACCCTTCTTGGA
  GCTTGTGTGGCAAACTGTGGAAAGATATTTCATTTAGAAGTATGTTCCCGTGATTTTGCAACGGAAGTAC
  GTTCTGTGATAAAAAATAAGGCTCATCCTAAAGTATGTGAAAAACTAAAATCTCTAATGGTGGAATGGTC
  AGAAGAATTCCAGAAGGACCCCCAATTTAGTCTGATATCTGCAACTATTAAGTCTATGAAAGAAGAAGGG
  GTTACTTTTCCTTCCGCAGGCTCCCAGACTGTCGCGGCTGCTGCCAAAAACGGGACATCATTGAACAAAA
  ACAAAGAAGATGAGGACATAGCTAAAGCTATTGAGTTATCGTTGCAAGAGCAGAAGCAGCAGTACACGGA
  GACAAAGGCTTTGTACCCACCCGCAGAGAGTCAGCTCAATAACAAGGCTGCACGGAGAGTCAGAGCTTTA
  TACGACTTTGAAGCTGTTGAGGACAACGAGCTCACCTTTAAACATGGTGAGCTCATTACTGTTTTGGATG
  ACAGTGACGCCAACTGGTGGCAAGGAGAAAATCACAGAGGAACAGGACTCTTCCCCTCCAACTTTGTAAC
  GACTGACTTAAGCACAGAGGTTGAGACAGCAACGGTGGACAAATTGAATGTAATTGATGATGATGTGGAG
  GAAATTAAGAAATCAGAACCTGAGCCTGTTTATATAGATGAGGGTAAAATGGATAGAGCCCTGCAGATTC
  TCCAGAGCATAGATCCAAAAGAGTCAAAACCCGACTCCCAAGACCTCTTGGACTTGGAAGATGTTTGCCA
  ACAGATGGGTCCGATGATAGATGAAAAACTTGAAGAGATTGATAGGAAGCATTCAGAACTGTCTGAGTTG
  AACGTAAAGGTGCTGGAAGCCCTGGACCTGTACAATAAGTTGGTGAATGAAGCGCCTGTGTACTCGGTCT
  ATTCAAAGCTGCATCCCGCACATTACCCACCCGCAGCAGCTGGGGTTCCAGTGCAGACATATCCAGTCCA
  GTCGCATGGTGGAAACTACCTGGGCCACGGCATTCACCAAGTATCTGTTGCCCAGAACTATAACCTAGGA
  CCTGATCCTATGGGCTCGCTGAGATCTCTGCCTCCAAATATGAACTCGGTAACAGCACACACCGTCCAAC
  CTCCATACTTAAGCACTGGACAGGACACTGTCTCCAACCCTTCTTACATGAACCAGAGCTCTCGTCTTCA
  GGCAGCTGCTGGTACAGCTGCTTACACACAGCCTGTGGGGATGTCTACAGATGTGTCTTCTTTCCAGAAC
  ACAGCATCCGGTTTGCCTCAGCTGGCTGGCTTTCCAGTGGCAGTTCCTGCACCTGTCGCTGCACAGCCAC
  AAGCAAGCTACCACCAGCAGCCTCTCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208368 representing NM_019667
  Red=Cloning site Green=Tags(s)
  
  MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDRVGSTPSGAKDCLKAIMKRVNHKVPHVALQALTLLG
  ACVANCGKIFHLEVCSRDFATEVRSVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEG
  VTFPSAGSQTVAAAAKNGTSLNKNKEDEDIAKAIELSLQEQKQQYTETKALYPPAESQLNNKAARRVRAL
  YDFEAVEDNELTFKHGELITVLDDSDANWWQGENHRGTGLFPSNFVTTDLSTEVETATVDKLNVIDDDVE
  EIKKSEPEPVYIDEGKMDRALQILQSIDPKESKPDSQDLLDLEDVCQQMGPMIDEKLEEIDRKHSELSEL
  NVKVLEALDLYNKLVNEAPVYSVYSKLHPAHYPPAAAGVPVQTYPVQSHGGNYLGHGIHQVSVAQNYNLG
  PDPMGSLRSLPPNMNSVTAHTVQPPYLSTGQDTVSNPSYMNQSSRLQAAAGTAAYTQPVGMSTDVSSFQN
  TASGLPQLAGFPVAVPAPVAAQPQASYHQQPLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019667
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019667.2, NP_062641.1
• RefSeq Size: 4124 bp
• RefSeq ORF: 1572 bp
• Locus ID: 56324
• UniProt ID: O88811
• Cytogenetics: 2 C1.1
• Gene Summary: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).[UniProtKB/Swiss-Prot Function]