Riok3 (NM_024182) Mouse Tagged ORF Clone
CAT#: MG208314
- TrueORF®
Riok3 (tGFP-tagged) - Mouse RIO kinase 3 (yeast) (Riok3)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_024182" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Riok3 |
Synonyms | 1200013N13Rik; D18Ertd331e; E130306C24Rik; Sudd |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208314 representing NM_024182
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGGTCGGAGTGTCTTCGCCTGAGCCAGGGCCCGCAGCAGCTTGGGGACCCAGTAAGTGCCCAT GGGCTACCCCTCAAAACACAGTATCCTGTTCACTGACCGAGGTGATGAGTGAGGAGCTGGCTAAAGAGCT GCAGTTGGAAGAAGAAGCGGCTGCTTTCCCTGAAGTTGTTGTTGCTGAAGGACCATTCATTTCTGGAGAA AACATTGACACTTCCAGTGACCTAATGCTGGCTCAGATGCTACAGATGGAATTTGACAGAGAATATGATG CACAGCTTAGGCGTGAGGAAAAGAAGTTCAATGGTGATAGCAAAGTTTCCATTTCGTTTGAAAACTATCG CAAAGTTCATCCTTTTGAAGACAGTGACAGCTCTGAAGACGAGGTTGACTGGCAGGACACTCGAGACGAT CCCTACAGACCAGCAAAGCCAATTCCTACTCCCAAAAAGGGTTTTATTGGAAAAGGAAAAGACATCACCA CCAAGCATGATGAAGTAGTATGTGGAAGAAAGAACACAGCAAGAATGGAAAATTTTGCCCCTGGCTTTCA GGTAGGGGATGGAATTGGAATGGATTTGAAACTATCCAACCACGTTTTTAATGCCTTAAAACAACACGCC TACTCAGAAGAGCGTCGGAGCGCCCGTCTCCACGAGAAGAAGGAACATTCTACCGCTGAAAAAGCAGTTG ATCCTAAGACACGCTTACTTATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTAT TAGTACAGGAAAGGAATCTGTTGTCTTTCATGCATATGGAGGGAGCCTGGAGGATGAAAAGGAAGATGGT AAGGCTATACCAACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAGTTTAAGAATCGTGACA AGTACATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAATCCACGTAAGATCATCCGCAT GTGGGCAGAGAAAGAAATGCACAATCTTACAAGAATGCAGAAGGCTGGAATTCCTTGTCCAACAGTTGTA CTGCTTAAGAAGCACATTTTAGTTATGTCTTTCATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAG AAGTAAAGCTTAGTAATGAAGAAATGAAAGATGCCTACTATCAGACTCTTCATTTGATGCAGCAATTATA TAACGAATGTACCCTTGTGCATGCTGACCTCAGCGAGTATAACATGTTGTGGCATGCTGGGAAGGTCTGG TTGATTGACGTCAGTCAGTCGGTAGAGCCAACCCATCCTCATGGCCTGGAGTTCTTATTCCGTGACTGTA GGAATGTTTCACAGTTCTTCCAGAAAGGAGGAGTAACGGAAGCCCTCAATGAACGGGAGCTGTTCAATGC TGTTTCTGGCCTGAACATCTCGGCAGACAATGAAGCTGATTTCTTAGCTGAGATAGAAGCTTTGGAGAAG ATGAATGAAGATCACGTTCAGAAGAACGGGAGGAAAGCAGCCTCGTTTCTGAAAGATGATGGAAGCCCAC CTGTGCTGTCTGCCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208314 representing NM_024182
Red=Cloning site Green=Tags(s) MDLVGVSSPEPGPAAAWGPSKCPWATPQNTVSCSLTEVMSEELAKELQLEEEAAAFPEVVVAEGPFISGE NIDTSSDLMLAQMLQMEFDREYDAQLRREEKKFNGDSKVSISFENYRKVHPFEDSDSSEDEVDWQDTRDD PYRPAKPIPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPGFQVGDGIGMDLKLSNHVFNALKQHA YSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSLEDEKEDG KAIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLTRMQKAGIPCPTVV LLKKHILVMSFIGHDQVPAPKLKEVKLSNEEMKDAYYQTLHLMQQLYNECTLVHADLSEYNMLWHAGKVW LIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVTEALNERELFNAVSGLNISADNEADFLAEIEALEK MNEDHVQKNGRKAASFLKDDGSPPVLSAD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024182 |
ORF Size | 1557 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024182.3, NP_077144.2 |
RefSeq Size | 3662 bp |
RefSeq ORF | 1560 bp |
Locus ID | 66878 |
UniProt ID | Q9DBU3 |
Cytogenetics | 18 6.15 cM |
Gene Summary | Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway. May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR208314 | Riok3 (Myc-DDK-tagged) - Mouse RIO kinase 3 (yeast) (Riok3) |
USD 484.00 |
|
MR208314L3 | Lenti ORF clone of Riok3 (Myc-DDK-tagged) - Mouse RIO kinase 3 (yeast) (Riok3) |
USD 784.00 |
|
MR208314L4 | Lenti ORF clone of Riok3 (mGFP-tagged) - Mouse RIO kinase 3 (yeast) (Riok3) |
USD 784.00 |
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