TNNT3 (C-term) Goat Polyclonal Antibody

CAT#: AP32711PU-N

TNNT3 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

TNNI3 Antibody (0.01 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TNNI3 Antibody staining of paraffin embedded Human Skeletal Muscle at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.01-0.03 μg/ml. Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1).
  The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).
  Immunohistochemistry on Paraffin Sections: 4-8 µg/ml. In paraffin embedded Human Skeletal Muscle shows staining of select fibrils.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_006748.1; NP_001036246.1; NP_001036247.1.
• Specificity: This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: troponin T3, fast skeletal type
• Database Link:
  Entrez Gene 7140 Human
  UniProt ID P45378
• Synonyms: TnTf, Beta TnTF, TNNT3