Sphkap (NM_172430) Mouse Recombinant Protein

CAT#: TP518656

Purified recombinant protein of Mouse SPHK1 interactor, AKAP domain containing (Sphkap), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR218656 representing NM_172430
  Red=Cloning site Green=Tags(s)
  
  MDVNSRLSVQSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGF
  VEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQAD
  FDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEE
  DFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTK
  TEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKD
  VLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFS
  MRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQS
  ALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMT
  QVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARP
  DAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCG
  TLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLV
  EDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRA
  STDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYG
  ITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKH
  KPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCS
  RTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVK
  QMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKE
  SKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQ
  PVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQ
  VEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAG
  LNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSR
  DSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSELVEEKEI
  LKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIE
  KFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 182.5 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_766018
• Locus ID: 77629
• UniProt ID: E9PUC4
• Cytogenetics: 1 C5
• Refseq Size: 6512
• Refseq ORF: 4974
• Synonyms: 4930544G21Rik; A930009L15Rik; AI852220; mKIAA1678; SKIP