Sphkap (NM_172430) Mouse Tagged ORF Clone

CAT#: MR218656

Sphkap (Myc-DDK-tagged) - Mouse SPHK1 interactor, AKAP domain containing (Sphkap)

Product Images

Circular map for MR218656

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sphkap
• Synonyms: 4930544G21Rik; A930009L15Rik; AI852220; mKIAA1678; SKIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218656 representing NM_172430
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTCAACTCCCGGCTTTCTGTGCAAAGCAACGTGGAATCACCACTGATGCATGAAGGTTCAGAGC
  CACAGCAGATCACCAGCAGTGCAGCAGGCAACCTGGCTGGCTCCATCACGGCCTGTAAAAAGGTTCTTCG
  AAGCAATAGCTTGCTGGAGTCCACAGACTACTGGCTGCAGAACCAGAGGACGCCTTGCCAGATTGGGTTT
  GTGGAAGATGAGTCAGAAAACTGTGCTTCTGTCTGCTTTGTGAACCTGGATGTCAACAAAGATGCCTGCA
  TCACAGAGAACCTGCAGCAGAAACTGGTCAATGTTTCACCGGACCTTCCAAACCTCATCAGCTCCATGAA
  TGTCCAACAGCCGAAAGAAAATGAAATTGTCCTCCTAAGTGGGTTAGCCTCTGGAAACCTCCAGGCAGAT
  TTTGATGTTTCACAGTGCCCTTGGCTACCAGATATCTGCTTGGTCCAGTGTGCAAGAGGGAACAGACCAA
  ACAGTACCAACTGCATCATCTTTGAGATCAACAAGTTCTTAATTGGTCTGGAAGTAGTGCAGGAGCGTCA
  GCTCCACCTGGAGACGAACGTGTTGAAGCTGGAGGATGATACAAACTGTTCTCTGTCTTCAATTGAAGAA
  GATTTTCTTACAGCCTCCGAGCATTTGGAGGAAGAAATCGAGGTGGACGACTGCAGAAGTGGTTTGGAAA
  ATACAAATGTGTCAGCTAATGTTTTGGAAAGTAAAAAGCCAAAGGAAACTACCCAGGAAGGATGGGATTA
  CCACAAGGAAAAGTTGCATTGTGCTTTAGGGGAAAAACACATTAGAAAACACCGTACGCCCTCCACAAAA
  ACAGAAGGGTCAAAGGAAAATACAGAAGAGAACACGTCCTTGAAAAGTCTCAATCGTTTAGTCAGGCCAT
  CACACCTGAAAAGTGAAGTGGCGGGGAACAAGCAACTGGCCACAAACTACTCCTACCCAGAAAATATCAA
  AGGGGAACTAGAAACATCTCAAATGCTGTTCATCCCTAGAGATGCCTATCTCTCCATGGTTAAGAAAGAT
  GTACTTTCTCCGTGTAGTGTACTCAGTGAGCAGGGAGGCAGCCACAGAGACCATGACGTTACTCCAAACC
  CTCTTCCTCCCGTTCAAAATGGAGAAGCCAGCACCGGAGAGTATGCCACAAACTTAGCAGAATCTGTAAT
  GCAAGATGCCTTCATCCGATTGTCTCAGTCACAGCCCACACTCCCCCAGGAATCTGCAGTCAGTTTCTCT
  ATGCGAAGTGCTCTCCTTCCCAGTGGCTGTTGCACAAAAGATATGGTGGTCCCTCGTTCATGGAACGAGC
  TACCCAAAATTGTCATTGTGCAGAGCCCAGATGGCAGTGACACAGTCCCTGAGCCAAATGTCTCCTCCTG
  GCCTGACATGGAATTTGTTGAAACCTCGGGCATCTTTTCTGCAGACAGTTCCAGCAGACCCACTCAGAGT
  GCCCTAGAAGTTGCATTGGCTTGTGCAGCCACTGTGATTGGAACCATTTCCAGTCCACAGGCCACAGAAA
  GATTCGCCATGGAGCAAGAATCCCTGGTATCTACCTATGCCCAGAGAGGCACTGGGGTACAGCAAACTCA
  AGTGCCCCAAGCATTCATGGCACCTTCTACAACCGAGTATTCCTTCCCATCAGCTCTGTGTGGCATGACA
  CAAGTGGCAAGTGCTGTCGCTGTCTGTGGCTTATGTGAAAAAGAAGAGGCGACATGTCCTGTAGCTCCAA
  CTGACCTCTTGCCTACATCTGGGGCTTCTGAAGAAATATCCTCCATCGGAAGTTTAGTGATGGAGAGGAG
  CACAGAACTGGGGAAGGAAGCCATTGCAGAGGCTCTGCTCAGAGAGGCTACTCTGATTTTAGCAAGGCCA
  GATGCTTACAGCAGCCTTGGTGAGCTCTTGGAATCTGTGAACCAGAGAATCATAGAAACCACTTCTAAAA
  CCCAGACCCTGTGCACAGAAAGTGTTCAAAGGAATGAACTGGCACACACCTTGTCCAATGTCATCCTCAA
  GCACTCCGTGGATGAACTTCACCAGAAAACTACAATGGCTCACCCCACTGATGAAAGGCATCCCTGTGGA
  ACTCTGGACACCTTGATGGAAAGTGTGAACCAGCTGCTGCACAATGTGATCTGCTTCACATTCAAAAAGA
  TGAATCACATTGTAACACTCAGTGAGCATCCCTCATTTGATCAGGCTGCTGGTCAAGCCTGGGTAAAAGC
  CTTTGCATGCCCCAGCAGCCAGCCTCTTAGCAATGCACACGGTACTGGCCTTGTCATCAGGAATCTTGTA
  GAGGATGCATCTCCTAAGTCAAACAAGGGCGGAGCAAGGCCAGAACTGGTCAACAACCCCAGGTTGCAAT
  CTGAATTCTCCTGCAGTCATAGAATGTTTGATTCAACTGCTAAGTCATTCCCCAAGGAAATATATCTGAA
  AGGGATTATGGGAGAGGATACCAGGAACCCTCATCATACACTAAATTATGACAGCAATGAACGAAGAGCC
  TCTACAGACCTAGGAAAATTGACAACAGCAAGCGAGGGTTGTAGTGGTTTCCAGGAAACTGAAGACAGCA
  TTGTTCCAAACACCCAAGAGAAATACATCTGTGCCACACCCCTAAACAATGAAGCTCAAGTTAACCTATC
  CTTATTAGGTGATGACCTGTCTGTTCCTGCTCAGTCTACTCTAGAGGCAAAGCAGTCCGAGGTCTATGGC
  ATCACAGATTTTGCGGAAGAATTGGCAGAGACTGTTGTCTCCATGGCAACTGAAATTGCAGCAATCTGCC
  TTGACAACTCCAATGGCAAACAGCCCTGGTTTTGTGCTTGGAAAAGAGGGAACGAGTTTTTAACGGCGCC
  TAACGGATCCTGCCGATCCTTGAAGAGGAAGAAGGAAAACTCAAGCGCCGGAAGCACCGTGAGGAAACAC
  AAGCCACCTCGCCTCAGTGAAATCAAGAGAAAAGCTGATGAGCACCCAGAGCTGAAAGAGAAGCTGATGA
  ACAGAGTCATGGATGAGTCCATGAACCTCGAAGACATCCCTGATTCCGTCAGTACCTTTGCCAATGAAGT
  GGCAGCCAAGATCATGAACCTCACAGAGTTTTCCATGGTGGATGGGGTGTGGCAAGGCCAGAGTTGTTCC
  CGGACTCGGCTTCTGGGTGGCGATAGGTGGAACCGGCTGAAGGCCTCAAGCTGTGAGAGCATTCCTGAGG
  AGGACTCTGAAGCCAGGGTCTTTGTAAACAGCCTGGGTTTGATGAGTACCTTAAGCCAACCAGTTAGCAG
  GGCCAGCTCCGTCTCCAAACAGTCCAGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAAA
  CAGATGGAAAACGAAGGGAGAGGGTTTGAGTTGCTGCTGGATTACTACGCAGGCAAAAATGCCAGCAGTA
  TCATGAGCTCAGCGATGCAGCAGGCATGCCAGAAAAACGACCACCTCAACGTGAGACCGAGCTGCCCCTC
  TAAGCAATCCAGTACAGAGAGCATAACAGAGGAGTTCTATAGGTACATGTTAAGGGACATCGCGAAAGAA
  AGCAAAGATGGTGCCTCCTCCAGACGTAGCAGCCATGATTGGACCACGGGCCTGCTGTCTCCTTCTACAC
  GATCCCCTCTGTGTTACAGACAGTCATCTATGCCTGACAGCAGGTCGCCATGCTCCAGACTAACAGTAAA
  TGCGCCTGTCAAAGCCAACTCCTTAGATGGCTTTGCCCAAAACTGCCCTCAAGATTCTGTAAATGTACAG
  CCAGTCAGTAGGGCTTCCTCATCTGGCCTCTGCAAATCAGATTCCTGCTTGTATCGCAGAAGTGGGACTG
  ACCAGATCACAAACATGCTAATTCATGAAACATGGGCCAGCTCCATTGAGGCTCTCATGAGAAAGAACAA
  AATCATTGCTGATGATAGCGAGGCAGCTAATGCCAGTCCTGGCCCTGTTTCCAGTGGTTCTCCTTTGCAA
  GTAGAGAAGAATGCCAACAGATTAGCCACCAGCAAAGGACACAGGGGGCCAACTCTGCTTGTACAGGAAT
  CTGTTGATTACCAGAGAAAAGATGCTGTTACTGAAGGCAATCATTCCCCAGTGTCATCTCCAGGCAAAAC
  AGCTCCTGTTAAAAAACCCAGTGATTTTGATCCTAGAAGAGAAACCTCTGCATGCCACAATGCTGCTGGT
  CTCAACAGCCCTAGGCGGTCACTCTGCTCAAGGGATGTGCCTTTGATTCAGATAGAGACAGATCAGAAAG
  AAGAGTGCATTGGAGAACCTGGACCTTTCCTTTCCCAAAGTGGCTCCCTAGAGGAAACAGAAGGCCACCA
  ACCTGAAGAAACCATCCCAGATGTGGCCAGAAATGAAGACACAGCTCCGAGCACCTGTCAGAGCTCTCGT
  GACAGCCTTGAGACCAGTGGGGAAGTAGAAGTGGAGGTCTTGAAAGAAGACATACCCCGAGATGAGTCCC
  GGAACCCTCCTAGCAGCAGCGAGGAGAGTACAGGCAGCTGGTCCCAGCTGGCCAATGAGGAGGACATCCC
  AGACGACACAAGCAGCTTTCTGCAGCTCAGCGAGCGGTCCATGAGTGAATTAGTAGAAGAAAAGGAGATT
  CTTAAAGAACAATCAGAAAGCATAAAGGAACATGCCTCTGGACTGCCAGGGAGAGCTGCCAGCCCCCAGA
  GGAGCCTACTGGTGATCAACTTTGACCTGGAGCCAGAGTGTCCTGATGCTGAGCTTCGAGCCACTCTGCA
  GTGGATAGCTGCCTCCGAACTGGGGATCCCAACAATCTACTTTAAGAAATCTCAGGAAAGCAGAATTGAA
  AAGTTTCTAGATGTTGTGAAGCTTGTTCAGCAGAAGTCCTGGAAGGTGGGAGATATATTTCACGCGGTTG
  TCCAGTACTGCAAACTGCATGCAGAGCAGAAGGAGAGGACTCCGAGTCTCTTTGACTGGCTTCTGGAACT
  AGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218656 representing NM_172430
  Red=Cloning site Green=Tags(s)
  
  MDVNSRLSVQSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGF
  VEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQAD
  FDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEE
  DFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTK
  TEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKD
  VLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFS
  MRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQS
  ALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMT
  QVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARP
  DAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCG
  TLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLV
  EDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRA
  STDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYG
  ITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKH
  KPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCS
  RTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVK
  QMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKE
  SKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQ
  PVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQ
  VEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAG
  LNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSR
  DSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSELVEEKEI
  LKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIE
  KFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172430
• ORF Size: 4974 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172430.4
• RefSeq Size: 6512 bp
• RefSeq ORF: 4977 bp
• Locus ID: 77629
• Cytogenetics: 1 C5
• MW: 182.5 kDa