CCN1 (CYR61) Human Gene Knockout Kit (CRISPR)

CAT#: KN203492

CYR61 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: CCN1
• Locus ID: 3491
• Components:

  KN203492G1, CCN1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACGACTAAGGCGAGCGCCC

  KN203492G2, CCN1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AACTCACCAGCCTGGTCAAG

  KN203492D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CACGCCCCAA AGAACCCTTC CCAACATAAG TCGTAATTTA AGGTGGAAAA AACGAACTGT TTTCTTGACG GGTCTGGGAC ACACACACAC ACACACACAC ACACACACAC ACCCCCCGAA CTGTTTTCTT GACGGGTCTG GGAGACAGAC ACACACACAG ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACAAAGGT GCAATGGAGC CAGGGGAGGC GCTTGGCAGC AGCCCGCGCC AACCAGCATT CCTGAGATGT TTGAGAATTC TGGAACGCGC AGACAGAGCC GACGTCACTG CAACACGCGG CGCCTCCGCC GGCCCGTATA AAAGGCGGGC TCCGGGGCGC CTGGGCAGAC CGCGAGCGAG AGCGCCCCCG AGCAGCGCCC GCGCCCTCCG CGCCTTCTCC GCCGGGACCT CGAGCGAAAG ACGCCCGCCC GCCGCCCAGC CCTCGCCTCC CTGCCCACCG GGCCCACCGC GCCGCCACCC CGACCCCGCT GCGCACGGCC TGTCCGCTGC ACACCAGCTT GTTGGCGTCT TCGTCGCCGC GCTCGCCCCG GGCTACTCCT GCGCGCCACA CCCCTTCCCC TGGTCCCAGA TTGCCCACGG CAGGAAAAGT TAAAAAGTTC GCGATCGTTT GCGGGTAGCC GTTTCTTTAA GCACTCTCCC CCTCCCCCCG AAGACGTGTC GGGACCTCTT GGTGGGAGCA GCCTTCCGAG GTGGCCGGGC TGGACGAGAT CAGAGGCTCC CCGTCGATAG GGTCGGAGAC CCCCGTCCCT CACTGCGGCA GCCGCGGCGC CCCTCCTGCG CACCGCGCCG AGTCTCACGC GTATCTTCTC CCCCTTCCAG GCGCTCTCCA CCTGCCCCGC TGCCTGCCAC TGCCCCCTGG AGGCGCCCAA GTGCGCGCCG GGAGTCGGGC TGGTCCGGGA CGGCTGCGGC TGCTGTAAGG TCTGCGCCAA GCAGCTCAAC GAGGACTGCA GCAAAACGCA GCCCTGCGAC CACACCAAGG GGCTGGAATG CAACTTCGGC GCCAGCTCCA CCGCTCTGAA GGGGATCTGC AGAGGTAAGA TGCTTGTGGT TTGGCCCCTT TAAAAAAAAT ACTAGTCCCC ATAGTCCAGA AGTTTAGTAA TTCTGAGACC ATGTATGGTG ATGCTTTGTT GTTGGTAGCT TGGCAGAAAG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001554
• UniProt ID: O00622
• Synonyms: CCN1; GIG1; IGFBP10
• Summary: The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]