Human TEX264 activation kit by CRISPRa

CAT#: GA109769

TEX264 CRISPRa kit - CRISPR gene activation of human testis expressed 264, ER-phagy receptor

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: TEX264
• Locus ID: 51368
• Kit Components:

  GA109769G1, TEX264 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA109769G2, TEX264 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA109769G3, TEX264 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001129884, NM_001243725, NM_001243726, NM_001243727, NM_001278195, NM_015926, NR_024012, NR_103462, NR_103463
• UniProt ID: Q9Y6I9
• Synonyms: ZSIG11
• Summary: Major reticulophagy (also called ER-phagy) receptor that acts independently of other candidate reticulophagy receptors to remodel subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537). The ATG8-containing isolation membrane (IM) cradles a tubular segment of TEX264-positive ER near a three-way junction, allowing the formation of a synapse of 2 juxtaposed membranes with trans interaction between the TEX264 and ATG8 proteins (PubMed:31006537). Expansion of the IM would extend the capture of ER, possibly through a 'zipper-like' process involving continued trans TEX264-ATG8 interactions, until poorly understood mechanisms lead to the fission of relevant membranes and, ultimately, autophagosomal membrane closure (PubMed:31006537).[UniProtKB/Swiss-Prot Function]