ACOT11 (NM_015547) Human Untagged Clone

CAT#: SC304326

ACOT11 (untagged)-Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACOT11
• Synonyms: BFIT; STARD14; THEA; THEM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015547 edited
  ATGATCCAGAATGTCGGAAATCACCTGCGACGGGGCTTGGCCTCTGTGTTCTCCAACCGC
  ACATCCCGGAAGTCAGCCTTACGTGCGGGGAACGACAGTGCCATGGCAGACGGCGAGGGA
  TACCGGAACCCCACGGAGGTGCAGATGAGCCAGCTGGTGCTGCCCTGCCACACCAACCAA
  CGTGGTGAGCTGAGCGTCGGGCAGCTGCTCAAGTGGATTGACACCACGGCTTGCCTGTCC
  GCGGAGAGGCACGCTGGCTGCCCCTGTGTCACAGCTTCCATGGATGACATCTATTTTGAG
  CACACCATTAGTGTTGGACAAGTGGTGAATATCAAGGCCAAGGTGAACCGGGCCTTCAAC
  TCCAGCATGGAGGTGGGCATCCAGGTGGCCTCGGAGGACCTGTGCTCTGAGAAGCAGTGG
  AATGTGTGCAAGGCCTTGGCCACCTTCGTGGCCCGCCGAGAGATCACCAAGGTGAAGCTG
  AAGCAGATCACGCCGCGGACAGAAGAGGAGAAGATGGAGCACAGTGTGGCGGCTGAGCGC
  CGGCGCATGCGCCTTGTCTATGCAGACACCATCAAGGACCTCCTGGCCAACTGCGCCATT
  CAGGGCGATCTGGAGAGCAGAGACTGTAGCCGCATGGTGCCGGCTGAGAAGACCCGTGTG
  GAGAGTGTGGAGCTGGTCCTGCCTCCCCACGCCAATCACCAGGGCAACACCTTTGGGGGC
  CAGATCATGGCCTGGATGGAGAATGTGGCCACCATTGCAGCCAGCCGGCTCTGCCGTGCC
  CACCCTACGCTGAAGGCCATTGAAATGTTCCACTTCCGAGGCCCGTCCCAGGTCGGCGAC
  CGTCTGGTGCTCAAAGCCATCGTGAACAATGCCTTCAAACATAGCATGGAGGTGGGCGTG
  TGCGTGGAGGCCTATCGCCAGGAGGCTGAGACCCACCGGCGCCACATCAACAGTGCCTTT
  ATGACCTTTGTGGTCCTGGACGCAGATGACCAGCCCCAGTTGCTGCCCTGGATTCGGCCC
  CAGCCCGGCGATGGTGAGCGGCGGTACCGAGAGGCCAGTGCCAGAAAGAAGATCCGCCTG
  GACAGGAAGTACATCGTGTCCTGTAAGCAGACAGAGGTGCCCCTCTCCGTCCCCTGGGAC
  CCTAGCAACCAGGTGTACCTGAGCTACAATAACGTCTCCTCCTTGAAGATGCTTGTGGCC
  AAGGACAACTGGGTGCTGTCCTCGGAGATCAGTCAGGTCCGCCTGTACACTCTGGAGGAT
  GACAAGTTCCTCTCCTTCCACATGGAGATGGTGGTGCATGTGGATGCAGCCCAGGCCTTC
  CTGCTGCTCTCGGACCTGCGTCAGAGGCCAGAGTGGGACAAGCACTACCGGAGCGTGGAG
  CTAGTGCAGCAGGTAGACGAGGACGACGCCATCTACCACGTCACCAGCCCTGCCCTCGGA
  GGTCACACAAAGCCCCAGGACTTCGTGATCCTGGCCTCGAGGCGGAAGCCTTGTGACAAT
  GGGGACCCCTATGTCATCGCGCTGAGGTCGGTCACGCTGCCCACACACCGAGAGACGCCA
  GAGTACAGACGCGGAGAGACCCTCTGCTCAGGCTTCTGCCTCTGGCGCGAGGGGGACCAG
  CTGACCAAGTGCTGCTGGGTTAGGGTCTCCCTGACTGAGCTGGTCTCGGCAAGTGGCTTC
  TATTCCTGGGGGCTCGAATCCAGGTCAAAGGGTCGCAGGAGCGACGGTTGGAATGGAAAA
  CTAGCTGGAGGACACCTGAGTACTCTTAAAGCAATCCCCGTGGCCAAAATCAACAGCCGA
  TTTGGATACCTTCAAGACACCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_015547
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015547.2, NP_056362.1
• RefSeq Size: 1921 bp
• RefSeq ORF: 1824 bp
• Locus ID: 26027
• UniProt ID: Q8WXI4
• Cytogenetics: 1p32.3
• Gene Summary: This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) encodes the longer isoform (BFIT1) of this protein.