ACOT11 (NM_015547) Human Tagged ORF Clone

CAT#: RG222250

ACOT11 (tGFP-tagged) - Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 1

Product Images

Circular map for RG222250

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACOT11
• Synonyms: BFIT; STARD14; THEA; THEM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222250 representing NM_015547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCAGAATGTCGGAAATCACCTGCGACGGGGCTTGGCCTCTGTGTTCTCCAACCGCACATCCCGGA
  AGTCAGCCTTACGTGCGGGGAACGACAGTGCCATGGCAGACGGCGAGGGATACCGGAACCCCACGGAGGT
  GCAGATGAGCCAGCTGGTGCTGCCCTGCCACACCAACCAACGTGGTGAGCTGAGCGTCGGGCAGCTGCTC
  AAGTGGATTGACACCACGGCTTGCCTGTCCGCGGAGAGGCACGCTGGCTGCCCCTGTGTCACAGCTTCCA
  TGGATGACATCTATTTTGAGCACACCATTAGTGTTGGACAAGTGGTGAATATCAAGGCCAAGGTGAACCG
  GGCCTTCAACTCCAGCATGGAGGTGGGCATCCAGGTGGCCTCGGAGGACCTGTGCTCTGAGAAGCAGTGG
  AATGTGTGCAAGGCCTTGGCCACCTTCGTGGCCCGCCGAGAGATCACCAAGGTGAAGCTGAAGCAGATCA
  CGCCGCGGACAGAAGAGGAGAAGATGGAGCACAGTGTGGCGGCTGAGCGCCGGCGCATGCGCCTTGTCTA
  TGCAGACACCATCAAGGACCTCCTGGCCAACTGCGCCATTCAGGGCGATCTGGAGAGCAGAGACTGTAGC
  CGCATGGTGCCGGCTGAGAAGACCCGTGTGGAGAGTGTGGAGCTGGTCCTGCCTCCCCACGCCAATCACC
  AGGGCAACACCTTTGGGGGCCAGATCATGGCCTGGATGGAGAATGTGGCCACCATTGCAGCCAGCCGGCT
  CTGCCGTGCCCACCCTACGCTGAAGGCCATTGAAATGTTCCACTTCCGAGGCCCGTCCCAGGTCGGCGAC
  CGTCTGGTGCTCAAAGCCATCGTGAACAATGCCTTCAAACATAGCATGGAGGTGGGCGTGTGCGTGGAGG
  CCTATCGCCAGGAGGCTGAGACCCACCGGCGCCACATCAACAGTGCCTTTATGACCTTTGTGGTCCTGGA
  CGCAGATGACCAGCCCCAGTTGCTGCCCTGGATTCGGCCCCAGCCCGGCGATGGTGAGCGGCGGTACCGA
  GAGGCCAGTGCCAGAAAGAAGATCCGCCTGGACAGGAAGTACATCGTGTCCTGTAAGCAGACAGAGGTGC
  CCCTCTCCGTCCCCTGGGACCCTAGCAACCAGGTGTACCTGAGCTACAATAACGTCTCCTCCTTGAAGAT
  GCTTGTGGCCAAGGACAACTGGGTGCTGTCCTCGGAGATCAGTCAGGTCCGCCTGTACACTCTGGAGGAT
  GACAAGTTCCTCTCCTTCCACATGGAGATGGTGGTGCATGTGGATGCAGCCCAGGCCTTCCTGCTGCTCT
  CGGACCTGCGTCAGAGGCCAGAGTGGGACAAGCACTACCGGAGCGTGGAGCTAGTGCAGCAGGTAGACGA
  GGACGACGCCATCTACCACGTCACCAGCCCTGCCCTCGGAGGTCACACAAAGCCCCAGGACTTCGTGATC
  CTGGCCTCGAGGCGGAAGCCTTGTGACAATGGGGACCCCTATGTCATCGCGCTGAGGTCGGTCACGCTGC
  CCACACACCGAGAGACGCCAGAGTACAGACGCGGAGAGACCCTCTGCTCAGGCTTCTGCCTCTGGCGCGA
  GGGGGACCAGCTGACCAAGTGCTGCTGGGTTAGGGTCTCCCTGACTGAGCTGGTCTCGGCAAGTGGCTTC
  TATTCCTGGGGGCTCGAATCCAGGTCAAAGGGTCGCAGGAGCGACGGTTGGAATGGAAAACTAGCTGGAG
  GACACCTGAGTACTCTTAAAGCAATCCCCGTGGCCAAAATCAACAGCCGATTTGGATACCTTCAAGACAC
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222250 representing NM_015547
  Red=Cloning site Green=Tags(s)
  
  MIQNVGNHLRRGLASVFSNRTSRKSALRAGNDSAMADGEGYRNPTEVQMSQLVLPCHTNQRGELSVGQLL
  KWIDTTACLSAERHAGCPCVTASMDDIYFEHTISVGQVVNIKAKVNRAFNSSMEVGIQVASEDLCSEKQW
  NVCKALATFVARREITKVKLKQITPRTEEEKMEHSVAAERRRMRLVYADTIKDLLANCAIQGDLESRDCS
  RMVPAEKTRVESVELVLPPHANHQGNTFGGQIMAWMENVATIAASRLCRAHPTLKAIEMFHFRGPSQVGD
  RLVLKAIVNNAFKHSMEVGVCVEAYRQEAETHRRHINSAFMTFVVLDADDQPQLLPWIRPQPGDGERRYR
  EASARKKIRLDRKYIVSCKQTEVPLSVPWDPSNQVYLSYNNVSSLKMLVAKDNWVLSSEISQVRLYTLED
  DKFLSFHMEMVVHVDAAQAFLLLSDLRQRPEWDKHYRSVELVQQVDEDDAIYHVTSPALGGHTKPQDFVI
  LASRRKPCDNGDPYVIALRSVTLPTHRETPEYRRGETLCSGFCLWREGDQLTKCCWVRVSLTELVSASGF
  YSWGLESRSKGRRSDGWNGKLAGGHLSTLKAIPVAKINSRFGYLQDT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015547
• ORF Size: 1821 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015547.4
• RefSeq Size: 1921 bp
• RefSeq ORF: 1824 bp
• Locus ID: 26027
• UniProt ID: Q8WXI4
• Cytogenetics: 1p32.3
• Gene Summary: This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]