Calbindin (CALB1) (NM_004929) Human Untagged Clone

CAT#: SC117035

CALB1 (untagged)-Human calbindin 1, 28kDa (CALB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Calbindin
• Synonyms: CALB; D-28K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117035 sequence for NM_004929 edited (data generated by NextGen Sequencing)
  ATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGATCTGG
  CTCCATTTCGACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTTGATC
  CAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAAAACT
  TTTGTGGATCAGTATGGGCAAAGAGATGATGGAAAAATAGGAATTGTAGAGTTGGCTCAC
  GTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGATGCCAGCAGCTGAAGTCCTGT
  GAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTTCATAGAAACT
  GAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGATGAC
  ACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGATGGG
  AAGCTGGAATTAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCTTAAA
  TTCCAGGGAATCAAAATGTGTGGGAAAGAGTTCAATAAGGCTTTTGAGCTGTATGATCAG
  GACGGCAATGGATACATAGATGAAAATGAACTGGATGCTTTACTGAAGGATCTGTGCGAG
  AAGAATAAACAGGATCTGGATATTAATAATATTACAACATACAAGAAGAACATAATGGCT
  TTGTCGGATGGAGGGAAGCTGTACCGAACGGATCTTGCTCTTATTCTCTGTGCTGGGGAT
  AACTAG
  
  Clone variation with respect to NM_004929.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004929 unedited
  GCGGCACGCGCAATTCGGCACGAGGCTGAGCTGAGCAGCACCGAGGACAGCGCCCGGCAG
  CGCCCGCGCCCAGGTCTCCCTCCGCAGCCCTGACTCGCGCACACGCTGAGCTTTTGCTCA
  CTCCCCCTCGCGCGGACACAGACACACGCATATTCACACACCCAGACACACACCCCGCTG
  TACAATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGAT
  CTGGCTCCATTTCGACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTT
  GATCCAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAA
  AACTTTTGTGGATCAGTATGGGCAAAGAGATGATGGAAAAATAGGAATTGTAGAGTTGGC
  TCACGTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGATGCCAGCAGCTGAAGTC
  CTGTGAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTTCATAGA
  AACTGAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGA
  TGACACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGA
  TGGGAAGCTGGAATTAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCT
  TAATTCCAGGGAATCAAAATGTGTGGGAAAAGAGTTCATAAGGGCTTTGAGCTGTATGAT
  CAGGACNNGCATGGATACATAGATGAAAATGAACTGGGATGCTTACTGAAGGATCTGTGC
  GAGAAGAATAACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004929 unedited
  GACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTAAGGTTATTTTCT
  TCTTTATTTATTCTTAGTAGTTAAATTCAGTCTAATGGCAGTGCTTCCATTTACACGCTA
  AAGGCAAAGCCAATCTCCCACTCAACATCATTTGTTGAATTTTAGTTTTTGGCAAATCTC
  GTTTCTGTTTGTTCTTGAGGGAAACATTATAGCTCACATTTTTTGGTTATTTTTATGAAT
  TCAGAAAATAAGTGTTGAATTTATAGAATGGGGACATTCAAGCTGACCCAGAACGATCAT
  TAACTCCTAGGCAGTAACTGTAAATTTCACATGGACACATCTCTCATAGCTCCCTGTTTC
  ACAATCAAAGAAATCTAAAATCACCAACTGTTACATATCAACTATAACCAGTATAGGATA
  TTCTAGCTACAAATATGTGAAACTGACTTAGATGTATGTTAAAGAATGTTTTAGAATCTT
  CTTGCTAGATAAGATAAATGATTACATTAGCATTTCTTTTCAGATGTTATTTTTTTAAAC
  TCATTTGAAACTATTTGCAGATAGAAATAGAACTATTTTGAATGCTGATCTCAATATGAA
  ATATAGAATCCACAGAATACACAAAAGTATTGATGATATAACATTCAATAATCAGGATGC
  TATGAATTTTACATTAAAATTTAATTATGTAGTAAAAAATANGAGTTGTTATAGCTAGAA
  AAAAATATTTTCAGAGGCAGCAGATACCCTTGGTGGAANTGAACANGTTCTCATAGAAAG
  GAAATCCCAGAAGTATTGCAACTAGACACAGGATATGCAGTATATAAAATTAATCAGAGA
  TACACAGGTTTGACATAAACAGCNGGTGACTGAATATTTTGCACACTGAAAACAGTTAGA
  AATGGATTAGGTCCTATCTCTGACGAAAATTGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004929
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004929.2, NP_004920.1
• RefSeq Size: 2531 bp
• RefSeq ORF: 786 bp
• Locus ID: 793
• UniProt ID: P05937
• Cytogenetics: 8q21.3
• Domains: EFh
• Gene Summary: The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]