TEX264 (NM_015926) Human Untagged Clone

CAT#: SC114528

TEX264 (untagged)-Human testis expressed 264 (TEX264), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TEX264
• Synonyms: ZSIG11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114528 sequence for NM_015926 edited (data generated by NextGen Sequencing)
  ATGTCGGACCTGCTACTACTGGGCCTGATTGGGGGCCTGACTCTCTTACTGCTGCTGACG
  CTGCTGGCCTTTGCCGGGTACTCAGGGCTACTGGCTGGGGTGGAAGTGAGTGCTGGGTCA
  CCCCCCATCCGCAACGTCACTGTGGCCTACAAGTTCCACATGGGGCTCTATGGTGAGACT
  GGGCGGCTTTTCACTGAGAGCTGCAGCATCTCTCCCAAGCTCCGCTCCATCGCTGTCTAC
  TATGACAACCCCCACATGGTGCCCCCTGATAAGTGCCGATGTGCCGTGGGCAGCATCCTG
  AGTGAAGGTGAGGAATCGCCCTCCCCTGAGCTCATCGACCTCTACCAGAAATTTGGCTTC
  AAGGTGTTCTCCTTCCCGGCACCCAGCCATGTGGTGACAGCCACCTTCCCCTACACCACC
  ATTCTGTCCATCTGGCTGGCTACCCGCCGTGTCCATCCTGCCTTGGACACCTACATCAAG
  GAGCGGAAGCTGTGTGCCTATCCTCGGCTGGAGATCTACCAGGAAGACCAGATCCATTTC
  ATGTGCCCACTGGCACGGCAGGGAGACTTCTATGTGCCTGAGATGAAGGAGACAGAGTGG
  AAATGGCGGGGGCTTGTGGAGGCCATTGACACCCAGGTGGATGGCACAGGAGCTGACACA
  ATGAGTGACACGAGTTCTGTAAGCTTGGAAGTGAGCCCTGGCAGCCGGGAGACTTCAGCT
  GCCACACTGTCACCTGGGGCGAGCAGCCGTGGCTGGGATGACGGTGACACCCGCAGCGAG
  CACAGCTACAGCGAGTCAGGTGCCAGCGGCTCCTCTTTTGAGGAGCTGGACTTGGAGGGC
  GAGGGGCCCTTAGGGGAGTCACGGCTGGACCCTGGGACTGAGCCCCTGGGGACTACCAAG
  TGGCTCTGGGAGCCCACTGCCCCTGAGAAGGGCAAGGAGTAA
  
  Clone variation with respect to NM_015926.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015926 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGAGCTGCCTT
  GAGGTGCAGTGTTGGGGATCCAGAGCCATGTCGGACCTGCTACTACTGGGCCTGATTGGG
  GGCCTGACTCTCTTACTGCTGCTGACGCTGCTGGCCTTTGCCGGGTACTCAGGGCTACTG
  GCTGGGGTGGAAGTGAGTGCTGGGTCACCCCCCATCCGCAACGTCACTGTGGCCTACAAG
  TTCCACATGGGGCTCTATGGTGAGACTGGGCGGCTTTTCACTGAGAGCTGCAGCATCTCT
  CCCAAGCTCCGCTCCATCGCTGTCTACTATGACAACCCCCACATGGTGCCCCCTGATAAG
  TGCCGATGTGCCGTGGGCAGCATCCTGAGTGAAGGTGAGGAATCGCCCTCCCCTGAGCTC
  ATCGACCTCTACCAGAAATTTGGCTTCAAGGTGTTCTCCTTCCCGGCACCCAGCCATGTG
  GTGACAGCCACCTTCCCCTACACCACCATTCTGTCCATCTGGCTGGCTACCCGCCGTGTC
  CATCCTGCCTTGGACACCTACATCAAGGAGCGGAAGCTGTGTGCCTATCCTCGGCTGGAG
  ATCTACCAGGAAGACCAGATCCATTTCATGTGCCCACTGGCACGGCAGGGAGACTTCTAT
  GTGCCTGAGATGAAGGAGACAGAGTGGAAATGGCGGNGGCTTGTGGAGGCCATTGACACC
  CAGGTGGATGGCACAGGAGCTGACACANTGAGTGACACGAGTTCTGTTAGCTTGGAAGTG
  AGCCCTGNCAGCCGGGAGACTTCAGCTGCCACACTGTCACCTGGGGCGAGCAGCCGTGGC
  TGGGATGACGGTGACACCCGCAGCGAGCACAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015926 unedited
  NTTTTACTCTGGACCGCGGCCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAG
  GTGAAACAAGTAAATCATTGGCTTTATTCTGGGTCCTGGAAGCTCCACTGTGAGTCTGAA
  AAAAAGACACAACAGGGGCGGCAGCCCTGGGGGCTGGTGCAGAAAATAGTCCCTGGCTCC
  TCTGGCCCTGGGAGCCTAAAGGGCAGTGAGGAGAAGGCTTAGCAAGAGGCCTGGAGCAGG
  GGAAGTCAGGTCCCTCAGGAACCCCTCCTCCCCCAGAGGAAGGAGGAAGAGGGCTGGAGA
  GTCTGCTGGAGAGTCTGCTCAGTTCCTCAGCAACTGCACTGCAGGAGGGTGCAGGCCATG
  GGTTACTCCTTGCCCTTCTCAGGGGCAGTGGGCTCCCAGAGCCACTTGGTAGTCCCCAGG
  GGCTCAGTCCCAGGGTCCAGCCGTGACTCCCCTAAGGGCCCCTCGCCCTCCAAGTCCAGC
  TCCTCAAAAGAGGAGCCGCTGGCACCTGACTCGCTGTAGCTGTGCTCGCTGCGGGTGTCA
  CCGTCATCCCAGCCACGGCTGCTCGCCCCAGGTGACAGTGTGGCAGCTGAAGTCTCCCGG
  CTGCCAGGGCTCACTTCCAAGCTTACAGAACTCGTGCCACTCATTGGGTCAGCTCCTGTG
  CCATCCACCTGGGTGTCAATGGCCTCCACAGCCCCCGCCATTTNCACTCTGGCTCCTTCA
  TCTAAGGCACATAGAAGTCTTCCTGCCGGGCCAGTGGGCACATGACATGGATCTGGCCTT
  CCTGGGAATACTTCAACCCGAGGATAGGCAACCCGCCTCCCGTTTCTTGATGGAGGTGCT
  CAAAGCCAGAATGCACACGCGGCGTACTCACCCCTATGCACAGATTGCTCGTGTCGGGCG
  ATGGTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015926
• Insert Size: 1370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015926.3, NP_057010.1
• RefSeq Size: 1601 bp
• RefSeq ORF: 942 bp
• Locus ID: 51368
• UniProt ID: Q9Y6I9
• Cytogenetics: 3p21.2
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: Major reticulophagy (also called ER-phagy) receptor that acts independently of other candidate reticulophagy receptors to remodel subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537). The ATG8-containing isolation membrane (IM) cradles a tubular segment of TEX264-positive ER near a three-way junction, allowing the formation of a synapse of 2 juxtaposed membranes with trans interaction between the TEX264 and ATG8 proteins (PubMed:31006537). Expansion of the IM would extend the capture of ER, possibly through a 'zipper-like' process involving continued trans TEX264-ATG8 interactions, until poorly understood mechanisms lead to the fission of relevant membranes and, ultimately, autophagosomal membrane closure (PubMed:31006537).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 2, 4, 5, and 7 encode the same isoform.