Gls2 (NM_001270787) Rat Tagged ORF Clone

CAT#: RR216945

Gls2 (myc-DDK-tagged) - Rat glutaminase 2 (liver, mitochondrial) (Gls2), transcript variant 2

Product Images

Circular map for RR216945

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gls2
• Synonyms: Ga
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216945 representing NM_001270787
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTTCCTTTAGATGTGGGGCTTTGGGTCCGCCGCGCTCGGGCGGTCAGGTGCTGTCCGCCCGGGA
  GGGCGTTTGGGCAGGTCGGTCTTCGAGATGATGCCTGTCCAAGTGATGCCTCGAACAGTGGCATGTTGCC
  TCGACTTGGTGACCTGCTTTTCTACACCATTGCAGAGGGGCAGGAACGCATCCCCATCCATAAGTTCACC
  ACGGCCCTGAAGGCCACTGGACTGCAGACGTCAGACCCACGGCTCCAGGACTGCATGAGCAAGATGCAGC
  GCATGGTCCAAGAGTCCAGCAGTGGTGGCCTCTTGGACCGAGAACTCTTCCAAAAGTGTGTGAGCAGCAA
  CATTGTGCTCCTGACTCAGGCATTCCGGAAGAAGTTTGTCATTCCTGACTTTGAGGAGTTCACAGGCCAT
  GTGGATCGTATATTTGAGGATGCCAAAGAGCTCACTGGAGGCAAAGTGGCAGCCTACATCCCTCACCTGG
  CCAAGTCAAACCCAGATCTTTGGGGCGTCTCCCTGTGCACTGTGGATGGGCAGCGACACTCTGTGGGCCA
  CACGAAGATCCCTTTCTGCCTACAGTCCTGTGTCAAGCCACTCACTTACGCCATCTCCGTGAGCACCTTA
  GGCACCGACTACGTGCACAAGTTCGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACTCTCCCTCA
  ACGAGGAAGGAATCCCTCATAACCCCATGGTCAATGCTGGTGCTATTGTGGTCAGCTCCCTGATCAAGAT
  GGACTGTAACAAAGCAGAGAAGTTTGACTTTGTGTTACAGTATCTGAACAAGATGGCTGGGAATGAATTC
  ATGGGGTTCAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCATTGGCTATT
  ACCTCAAGGAGAAGAAGTGCTTCCCTAAGGGAGTGGACATGATGGCTGCCCTTGATCTCTATTTCCAGCT
  GTGCTCTGTGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTTGCCAATGGCGGCATCTGC
  CCCATCACAGGGGAGAGCGTGCTGAGTGCGGAAGCCGTGCGCAACACCCTCAGCCTCATGCACTCCTGTG
  GCATGTATGACTTCTCGGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTGTCGGGAGC
  CATCCTCCTGGTTGTACCCAATGTCATGGGGATGATGTGTCTGTCACCCCCGTTAGACAAGCTGGGGAAC
  AGCCACAGGGGCATCAGCTTCTGCCAGAAGTTGGTGTCTCTGTTTAACTTCCACAACTACGACAACCTGC
  GGCACTGTGCTCGGAAGTTAGACCCACGGAGGGAAGGGGGGGAAGTTCGGAACAAGACCGTGGTGAACCT
  GTTATTTGCTGCATATAGTGGAGATGTCTCAGCTCTTCGAAGGTTTGCCTTGTCTGCCGTGGATATGGAG
  CAGAAGGACTATGATTCCCGCACAGCCCTACATGTGGCGGCAGCGGAAGGACACATTGACGTTGTCAAGT
  TTCTGATCGAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGC
  CGTGCAGTTCAATCACCTGGAGGTGGTCAAACTGCTTCAGGATTACCATGACTCCTACATGCTGTCTGAG
  ACTCAAGCTGAGGTAGCAGCTGAGACTCTGTCAAAAGAGAACTTAGAGAGCATGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216945 representing NM_001270787
  Red=Cloning site Green=Tags(s)
  
  MPFPLDVGLWVRRARAVRCCPPGRAFGQVGLRDDACPSDASNSGMLPRLGDLLFYTIAEGQERIPIHKFT
  TALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEFTGH
  VDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISVSTL
  GTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEF
  MGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGIC
  PITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGN
  SHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAVDME
  QKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYMLSE
  TQAEVAAETLSKENLESMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270787
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270787.1, NP_001257716.1
• RefSeq Size: 2798 bp
• RefSeq ORF: 1740 bp
• Locus ID: 192268
• UniProt ID: P28492
• Cytogenetics: 7q11
• MW: 63.9 kDa
• Gene Summary: first enzyme of glutamine catabolism in liver [RGD, Feb 2006]