Gls2 (NM_001270787) Rat Untagged Clone

CAT#: RN216945

Gls2 (untagged) - Rat glutaminase 2 (liver, mitochondrial) (Gls2), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gls2
• Synonyms: Ga
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216945 representing NM_001270787
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTTCCTTTAGATGTGGGGCTTTGGGTCCGCCGCGCTCGGGCGGTCAGGTGCTGTCCGCCCGGGA
  GGGCGTTTGGGCAGGTCGGTCTTCGAGATGATGCCTGTCCAAGTGATGCCTCGAACAGTGGCATGTTGCC
  TCGACTTGGTGACCTGCTTTTCTACACCATTGCAGAGGGGCAGGAACGCATCCCCATCCATAAGTTCACC
  ACGGCCCTGAAGGCCACTGGACTGCAGACGTCAGACCCACGGCTCCAGGACTGCATGAGCAAGATGCAGC
  GCATGGTCCAAGAGTCCAGCAGTGGTGGCCTCTTGGACCGAGAACTCTTCCAAAAGTGTGTGAGCAGCAA
  CATTGTGCTCCTGACTCAGGCATTCCGGAAGAAGTTTGTCATTCCTGACTTTGAGGAGTTCACAGGCCAT
  GTGGATCGTATATTTGAGGATGCCAAAGAGCTCACTGGAGGCAAAGTGGCAGCCTACATCCCTCACCTGG
  CCAAGTCAAACCCAGATCTTTGGGGCGTCTCCCTGTGCACTGTGGATGGGCAGCGACACTCTGTGGGCCA
  CACGAAGATCCCTTTCTGCCTACAGTCCTGTGTCAAGCCACTCACTTACGCCATCTCCGTGAGCACCTTA
  GGCACCGACTACGTGCACAAGTTCGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACTCTCCCTCA
  ACGAGGAAGGAATCCCTCATAACCCCATGGTCAATGCTGGTGCTATTGTGGTCAGCTCCCTGATCAAGAT
  GGACTGTAACAAAGCAGAGAAGTTTGACTTTGTGTTACAGTATCTGAACAAGATGGCTGGGAATGAATTC
  ATGGGGTTCAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCATTGGCTATT
  ACCTCAAGGAGAAGAAGTGCTTCCCTAAGGGAGTGGACATGATGGCTGCCCTTGATCTCTATTTCCAGCT
  GTGCTCTGTGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTTGCCAATGGCGGCATCTGC
  CCCATCACAGGGGAGAGCGTGCTGAGTGCGGAAGCCGTGCGCAACACCCTCAGCCTCATGCACTCCTGTG
  GCATGTATGACTTCTCGGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTGTCGGGAGC
  CATCCTCCTGGTTGTACCCAATGTCATGGGGATGATGTGTCTGTCACCCCCGTTAGACAAGCTGGGGAAC
  AGCCACAGGGGCATCAGCTTCTGCCAGAAGTTGGTGTCTCTGTTTAACTTCCACAACTACGACAACCTGC
  GGCACTGTGCTCGGAAGTTAGACCCACGGAGGGAAGGGGGGGAAGTTCGGAACAAGACCGTGGTGAACCT
  GTTATTTGCTGCATATAGTGGAGATGTCTCAGCTCTTCGAAGGTTTGCCTTGTCTGCCGTGGATATGGAG
  CAGAAGGACTATGATTCCCGCACAGCCCTACATGTGGCGGCAGCGGAAGGACACATTGACGTTGTCAAGT
  TTCTGATCGAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGC
  CGTGCAGTTCAATCACCTGGAGGTGGTCAAACTGCTTCAGGATTACCATGACTCCTACATGCTGTCTGAG
  ACTCAAGCTGAGGTAGCAGCTGAGACTCTGTCAAAAGAGAACTTAGAGAGCATGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270787
• Insert Size: 1740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270787.1, NP_001257716.1
• RefSeq Size: 2798 bp
• RefSeq ORF: 1740 bp
• Locus ID: 192268
• UniProt ID: P28492
• Cytogenetics: 7q11
• Gene Summary: first enzyme of glutamine catabolism in liver [RGD, Feb 2006]
  Transcript Variant: This variant (2) uses an alternate splice site at the 3' end of the first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.