Mapkapk3 (NM_001012127) Rat Tagged ORF Clone

CAT#: RR204033

Mapkapk3 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase-activated protein kinase 3 (Mapkapk3), (10 ug)

Product Images

Circular map for RR204033

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapkapk3
• Synonyms: MAPKAP-K3; MK-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204033 representing NM_001012127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCGAGACGGCGGGGGAGAAGGGGAGTCTTGTGCCCCAGCCAGGTGCGCTCGGTGCGCCCGCCT
  TGGGCGGTGCTCCGGCCCCAGGTGTGCGACGGGAACCCAAGAAGTACGCGGTGACTGATGACTACCAGTT
  GTCCAAGCAAGTGCTGGGTCTGGGTGTGAACGGCAAGGTACTAGAGTGCTACCATCGGCGCTCTGGGCAG
  AAGTGTGCCTTGAAGCTCCTGTATGACAGCCCCAAGGCCCGGCAGGAGGTGGACCACCACTGGCAGGCCT
  CAGGCGGCCCCCACATTGTGCGCATCCTGGACGTGTACGAGAACATGCATCACGGCAAGCGCTGCCTCCT
  CATCGTCATGGAATGCATGGAGGGTGGTGAGCTGTTCAGCAGGATTCAGGAGCGCGGTGATCAGGCTTTC
  ACTGAGAGAGAGGCCGCAGAGATAATGCGGGACATTGGCACTGCCATCCAGTTCTTGCACAGCCAGAACA
  TTGCCCACCGAGATGTCAAGCCTGAAAACCTACTCTATACATCCAAGGAGAAGGATGCTGTACTTAAACT
  CACTGATTTTGGCTTTGCCAAGGAAACCACCCAAAATGCCCTCCAGACACCCTGTTACACTCCCTATTAT
  GTGGCTCCTGAGGTCCTGGGTCCAGAGAAGTATGACAAGTCGTGCGATATGTGGTCCCTGGGGGTCATCA
  TGTACATCCTTTTGTGTGGATTCCCACCCTTCTACTCCAACACGGGCCAGGCCATCTCTCCAGGAATGAA
  GAGAAGGATTCGCTTGGGCCAGTATGGCTTCCCTAAACCTGAATGGGCAGACGTCTCTGAGGATGCCAAG
  CAGCTAATCCGTCTGCTCCTGAAGACAGATCCCACCGAGAGGCTGACCATCATGCAGTTTATGAACCATC
  CTTGGATCAATCAATCGATGGAGGTCCCACAGACCCCACTCCATACAGCCCGAGTGCTGGAGGAAGACAA
  AGACCACTGGGATGACGTCAAGGAAGAGATGACCAGTGCCCTGGCCACGATGAGGGTAGACTACGACCAG
  GTGAAAATCAAGGACCTGAAGACCTCTAACAACCGACTCCTCAACAAGCGGAGGAAAAAGCAGGGGGGCA
  GCTCCTCAGCCTCACCGGGATGCAACAACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204033 representing NM_001012127
  Red=Cloning site Green=Tags(s)
  
  MDGETAGEKGSLVPQPGALGAPALGGAPAPGVRREPKKYAVTDDYQLSKQVLGLGVNGKVLECYHRRSGQ
  KCALKLLYDSPKARQEVDHHWQASGGPHIVRILDVYENMHHGKRCLLIVMECMEGGELFSRIQERGDQAF
  TEREAAEIMRDIGTAIQFLHSQNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYY
  VAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPKPEWADVSEDAK
  QLIRLLLKTDPTERLTIMQFMNHPWINQSMEVPQTPLHTARVLEEDKDHWDDVKEEMTSALATMRVDYDQ
  VKIKDLKTSNNRLLNKRRKKQGGSSSASPGCNNQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012127
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012127.1, NP_001012127.1
• RefSeq Size: 2634 bp
• RefSeq ORF: 1155 bp
• Locus ID: 315994
• UniProt ID: Q66H84
• Cytogenetics: 8q32
• MW: 43.2 kDa
• Gene Summary: Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression (By similarity).[UniProtKB/Swiss-Prot Function]