Nr1d1 (NM_145775) Rat Tagged ORF Clone

CAT#: RR200153

Nr1d1 (Myc-DDK-tagged ORF) - Rat nuclear receptor subfamily 1, group D, member 1 (Nr1d1), transcript variant 2, (10 ug)

Product Images

Circular map for RR200153

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nr1d1
• Synonyms: REV-ERBAALPHA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200153 representing NM_145775
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACAGCAGCCGAGTGTCCCCCAGCAAGGGCACCAGCAACATTACCAAGCTTAACGGCATGGTGC
  TACTGTGTAAAGTGTGTGGGGACGTGGCCTCAGGCTTCCACTATGGCGTGCATGCCTGCGAGGGCTGCAA
  GGGCTTTTTCCGCCGGAGCATCCAGCAGAACATCCAGTACAAGCGGTGTTTGAAAAACGAGAACTGCTCC
  ATTGTCCGTATCAATCGCAACCGCTGCCAGCAGTGTCGCTTCAAGAAGTGTCTCTCCGTTGGCATGTCTA
  GAGACGCTGTGCGTTTTGGACGTATCCCCAAGAGAGAGAAGCAACGAATGCTTGCTGAGATGCAGAACGC
  CATGAACTTGGCCAACAACCAACTGAGCAGCCTGTGCCCTCTAGAGACCTCACCTGCCCCGCACCCCACC
  TCAGGCTCCGTGGGCCCCTCACCACCTCCTGCACCAGCCCCGACACCTTTGGTGGGCTTCTCTCAGTTCC
  CACAACAGCTGACACCACCCAGATCCCCGAGTCCTGAGCCCACCGTGGAGGATGTGATATCCCAGGTGGC
  CAGGGCCCATCGAGAAATCTTCACCTATGCCCATGACAAATTAGGCACCTCACCTGGCAACTTCAATGCC
  AATCATGCATCAGGTAGCCCTCCGGCTACCACTCCACAGTGCTGGGAGAGTCAGGGATGCCCGTCTACCC
  CCAACGACAACAACCTTTTGGCGGCTCAGCGTCATAATGAAGCACTGAATGGTCTACGCCAGGGCCCCTC
  CTCCTACCCTCCTACCTGGCCTTCTGGCCCTGCCCACCACAGCTGCCACCAGCCTAACAGCAATGGGCAT
  CGCCTGTGCCCCACCCACGTATACTCGGCCCCAGAAGGCAAGGCACCTGCCAACGGTCTACGGCAAGGCA
  ACACCAAGAATGTTCTGCTGGCATGTCCCATGAACATGTATCCCCATGGACGTAGTGGCCGGACTGTGCA
  GGAGATCTGGGAAGACTTCTCTATGAGCTTCACACCCGCTGTGCGGGAGGTGGTAGAATTTGCCAAACAC
  ATCCCCGGCTTCCGTGACCTTTCTCAGCACGACCAGGTGACCCTGCTTAAGGCTGGCACCTTTGAGGTGC
  TGATGGTGCGCTTTGCGTCATTGTTCAACGTGAAGGACCAGACAGTCATGTTCCTGAGCCGCACAACCTA
  CAGCCTGCAGGAGCTCGGTGCCATGGGCATGGGTGACCTGCTCAATGCCATGTTTGACTTCAGCGAGAAG
  CTCAACTCTCTGGCGCTTACTGAGGAGGAGCTGGGCCTTTTCACGGCAGTGGTACTTGTCTCTGCAGACC
  GCTCGGGAATGGAGAATTCCGCTTCGGTGGAGCAGCTCCAGGAGACGCTGCTGCGGGCTCTTCGGGCTCT
  GGTGCTGAAGAACCGGCCCTCGGAGACTTCCCGCTTCACCAAACTGCTGCTCAAGCTGCCGGACCTGCGG
  ACCCTGAACAACATGCATTCCGAGAAGCTGCTGTCCTTCCGGGTGGACGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200153 representing NM_145775
  Red=Cloning site Green=Tags(s)
  
  MEDSSRVSPSKGTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCS
  IVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQNAMNLANNQLSSLCPLETSPAPHPT
  SGSVGPSPPPAPAPTPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGTSPGNFNA
  NHASGSPPATTPQCWESQGCPSTPNDNNLLAAQRHNEALNGLRQGPSSYPPTWPSGPAHHSCHQPNSNGH
  RLCPTHVYSAPEGKAPANGLRQGNTKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKH
  IPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLNAMFDFSEK
  LNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPSETSRFTKLLLKLPDLR
  TLNNMHSEKLLSFRVDAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145775
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145775.2, NP_665718.2
• RefSeq Size: 2314 bp
• RefSeq ORF: 1527 bp
• Locus ID: 252917
• UniProt ID: Q63503
• Cytogenetics: 10q31
• MW: 56 kDa
• Gene Summary: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (Arntl). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Feb 2014]