Nr1d1 (NM_145775) Rat Untagged Clone

CAT#: RN200153

Nr1d1 (untagged ORF) - Rat nuclear receptor subfamily 1, group D, member 1 (Nr1d1), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nr1d1
• Synonyms: REV-ERBAALPHA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200153 representing NM_145775
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACAGCAGCCGAGTGTCCCCCAGCAAGGGCACCAGCAACATTACCAAGCTTAACGGCATGGTGC
  TACTGTGTAAAGTGTGTGGGGACGTGGCCTCAGGCTTCCACTATGGCGTGCATGCCTGCGAGGGCTGCAA
  GGGCTTTTTCCGCCGGAGCATCCAGCAGAACATCCAGTACAAGCGGTGTTTGAAAAACGAGAACTGCTCC
  ATTGTCCGTATCAATCGCAACCGCTGCCAGCAGTGTCGCTTCAAGAAGTGTCTCTCCGTTGGCATGTCTA
  GAGACGCTGTGCGTTTTGGACGTATCCCCAAGAGAGAGAAGCAACGAATGCTTGCTGAGATGCAGAACGC
  CATGAACTTGGCCAACAACCAACTGAGCAGCCTGTGCCCTCTAGAGACCTCACCTGCCCCGCACCCCACC
  TCAGGCTCCGTGGGCCCCTCACCACCTCCTGCACCAGCCCCGACACCTTTGGTGGGCTTCTCTCAGTTCC
  CACAACAGCTGACACCACCCAGATCCCCGAGTCCTGAGCCCACCGTGGAGGATGTGATATCCCAGGTGGC
  CAGGGCCCATCGAGAAATCTTCACCTATGCCCATGACAAATTAGGCACCTCACCTGGCAACTTCAATGCC
  AATCATGCATCAGGTAGCCCTCCGGCTACCACTCCACAGTGCTGGGAGAGTCAGGGATGCCCGTCTACCC
  CCAACGACAACAACCTTTTGGCGGCTCAGCGTCATAATGAAGCACTGAATGGTCTACGCCAGGGCCCCTC
  CTCCTACCCTCCTACCTGGCCTTCTGGCCCTGCCCACCACAGCTGCCACCAGCCTAACAGCAATGGGCAT
  CGCCTGTGCCCCACCCACGTATACTCGGCCCCAGAAGGCAAGGCACCTGCCAACGGTCTACGGCAAGGCA
  ACACCAAGAATGTTCTGCTGGCATGTCCCATGAACATGTATCCCCATGGACGTAGTGGCCGGACTGTGCA
  GGAGATCTGGGAAGACTTCTCTATGAGCTTCACACCCGCTGTGCGGGAGGTGGTAGAATTTGCCAAACAC
  ATCCCCGGCTTCCGTGACCTTTCTCAGCACGACCAGGTGACCCTGCTTAAGGCTGGCACCTTTGAGGTGC
  TGATGGTGCGCTTTGCGTCATTGTTCAACGTGAAGGACCAGACAGTCATGTTCCTGAGCCGCACAACCTA
  CAGCCTGCAGGAGCTCGGTGCCATGGGCATGGGTGACCTGCTCAATGCCATGTTTGACTTCAGCGAGAAG
  CTCAACTCTCTGGCGCTTACTGAGGAGGAGCTGGGCCTTTTCACGGCAGTGGTACTTGTCTCTGCAGACC
  GCTCGGGAATGGAGAATTCCGCTTCGGTGGAGCAGCTCCAGGAGACGCTGCTGCGGGCTCTTCGGGCTCT
  GGTGCTGAAGAACCGGCCCTCGGAGACTTCCCGCTTCACCAAACTGCTGCTCAAGCTGCCGGACCTGCGG
  ACCCTGAACAACATGCATTCCGAGAAGCTGCTGTCCTTCCGGGTGGACGCCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145775
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145775.2, NP_665718.2
• RefSeq Size: 2314 bp
• RefSeq ORF: 1527 bp
• Locus ID: 252917
• UniProt ID: Q63503
• Cytogenetics: 10q31
• Gene Summary: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (Arntl). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) is shorter at the N-temrinus, as compared to isoform 1.