Hgf (NM_017017) Rat Untagged Clone

CAT#: RN213062

Hgf (untagged ORF) - Rat hepatocyte growth factor (Hgf), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hgf
• Synonyms: HPTA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213062 representing NM_017017
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGGGGGACCAAACTTCTGCCGGTCCTGTTGCTGCAGCATGTCCTGCTGCACCTCCTCCTGCTTC
  CTGTCACCATCCCCTATGCAGAAGGACAGAAGAAGAGAAGAAATACTCTTCATGAATTCAAAAAGTCAGC
  AAAAACTACTCTTACCAAGGAAGACCCATTAGTGAAGATTAAAACCAAAAAAGTGAACTCTGCAGATGAG
  TGTGCCAACAGGTGCATCAGAAACAAGGGCTTTCCATTCACTTGCAAGGCCTTTGTTTTTGATAAGTCGA
  GAAAACGATGCTACTGGTATCCTTTCAATAGTATGTCAAGTGGAGTGAAAAAAGGGTTTGGCCATGAATT
  TGACCTCTATGAAAACAAAGACTATATTAGAAATTGCATCATTGGTAAAGGAGGCAGCTATAAGGGGACA
  GTATCCATCACTAAGAGTGGCATCAAGTGCCAGCCTTGGAATTCCATGATCCCCCATGAACACAGCTTTT
  TGCCTTCGAGCTATCGCGGTAAAGACCTACAGGAAAACTACTGTCGAAATCCTCGAGGGGAAGAAGGGGG
  ACCCTGGTGTTTCACAAGCAATCCAGAGGTACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGTT
  GAATGCATGACCTGCAACGGTGAAAGCTACAGAGGTCCCATGGATCACACAGAATCAGGCAAGACATGTC
  AGCGCTGGGATCAGCAGACACCACACCGGCACAAATTCTTGCCGGAAAGATATCCCGACAAGGGCTTTGA
  TGATAATTATTGCCGCAATCCCGATGGCAAGCCGAGGCCATGGTGCTACACTCTTGACCCTGACACCCCT
  TGGGAGTATTGTGCAATTAAAATGTGCGCTCACAGTGCTGTGAATGAGACTGATGTTCCCATGGAAACAA
  CTGAATGTATAAAAGGCCAAGGAGAAGGTTACAGGGGAACCACCAATACCATTTGGAATGGAATTCCGTG
  TCAGCGTTGGGATTCGCAGTACCCCCACAAGCATGACATCACTCCCGAGAACTTCAAATGCAAGGACCTT
  AGAGAAAATTATTGCCGCAATCCGGATGGGGCTGAATCACCATGGTGTTTTACCACTGATCCAAACATCC
  GAGTTGGTTACTGCTCTCAAATTCCCAAATGTGACGTGTCAAGTGGACAAGATTGTTATCGTGGCAATGG
  GAAAAACTACATGGGCAACTTATCCAAAACAAGGTCTGGACTCACATGTTCCATGTGGGACAAGAATATG
  GAGGATTTACACCGTCATATCTTCTGGGAGCCAGACGCTAGCAAGTTGACTAAGAATTACTGCCGGAACC
  CCGATGACGACGCCCATGGACCTTGGTGCTACACAGGGAATCCTCTCGTTCCTTGGGATTATTGCCCTAT
  TTCCCGTTGTGAAGGAGATACTACACCTACAATTGTCAATTTGGACCATCCTGTAATATCCTGTGCCAAA
  ACAAAACAACTGCGAGTTGTAAATGGCATTCCAACACAAACAACAGTAGGGTGGATGGTTAGTTTGAAAT
  ACAGGAATAAACACATCTGTGGGGGATCATTGATAAAGGAAAGTTGGGTTCTTACTGCAAGGCAATGTTT
  TCCAGCTAGAAACAAAGACTTGAAAGACTATGAAGCTTGGCTTGGAATCCATGATGTCCATGAGAGAGGC
  GAGGAGAAACGCAAACAGATCTTAAACATTTCCCAGCTAGTCTATGGACCTGAAGGCTCAGATTTGGTTT
  TACTGAAGCTTGCTCGCCCTGCAATCCTGGATAACTTTGTCAGTACAATTGATTTACCTAGTTATGGCTG
  TACAATCCCTGAAAAGACTACTTGCAGTATTTACGGCTGGGGCTACACTGGATTGATCAACGCAGATGGT
  TTATTACGAGTAGCTCATCTGTATATTATGGGGAATGAGAAATGCAGTCAGCACCATCAAGGCAAGGTGA
  CTTTGAATGAGTCTGAATTATGTGCTGGGGCTGAAAAGATTGGATCAGGACCTTGTGAGGGAGATTATGG
  TGGCCCACTCATTTGTGAACAACACAAAATGAGAATGGTTCTTGGTGTCATTGTTCCTGGTCGTGGATGT
  GCCATCCCAAATCGTCCTGGTATTTTTGTTCGAGTAGCATATTATGCAAAATGGATACACAAAGTAATTT
  TGACATACAAGTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017017
• Insert Size: 2187 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017017.2, NP_058713.1
• RefSeq Size: 2532 bp
• RefSeq ORF: 2187 bp
• Locus ID: 24446
• UniProt ID: P17945
• Cytogenetics: 4q12
• Gene Summary: plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle [RGD, Feb 2006]