Hgf (NM_017017) Rat Tagged ORF Clone

CAT#: RR213062

Hgf (Myc-DDK-tagged ORF) - Rat hepatocyte growth factor (Hgf), (10 ug)

Product Images

Circular map for RR213062

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hgf
• Synonyms: HPTA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213062 representing NM_017017
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGGGGGACCAAACTTCTGCCGGTCCTGTTGCTGCAGCATGTCCTGCTGCACCTCCTCCTGCTTC
  CTGTCACCATCCCCTATGCAGAAGGACAGAAGAAGAGAAGAAATACTCTTCATGAATTCAAAAAGTCAGC
  AAAAACTACTCTTACCAAGGAAGACCCATTAGTGAAGATTAAAACCAAAAAAGTGAACTCTGCAGATGAG
  TGTGCCAACAGGTGCATCAGAAACAAGGGCTTTCCATTCACTTGCAAGGCCTTTGTTTTTGATAAGTCGA
  GAAAACGATGCTACTGGTATCCTTTCAATAGTATGTCAAGTGGAGTGAAAAAAGGGTTTGGCCATGAATT
  TGACCTCTATGAAAACAAAGACTATATTAGAAATTGCATCATTGGTAAAGGAGGCAGCTATAAGGGGACA
  GTATCCATCACTAAGAGTGGCATCAAGTGCCAGCCTTGGAATTCCATGATCCCCCATGAACACAGCTTTT
  TGCCTTCGAGCTATCGCGGTAAAGACCTACAGGAAAACTACTGTCGAAATCCTCGAGGGGAAGAAGGGGG
  ACCCTGGTGTTTCACAAGCAATCCAGAGGTACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGTT
  GAATGCATGACCTGCAACGGTGAAAGCTACAGAGGTCCCATGGATCACACAGAATCAGGCAAGACATGTC
  AGCGCTGGGATCAGCAGACACCACACCGGCACAAATTCTTGCCGGAAAGATATCCCGACAAGGGCTTTGA
  TGATAATTATTGCCGCAATCCCGATGGCAAGCCGAGGCCATGGTGCTACACTCTTGACCCTGACACCCCT
  TGGGAGTATTGTGCAATTAAAATGTGCGCTCACAGTGCTGTGAATGAGACTGATGTTCCCATGGAAACAA
  CTGAATGTATAAAAGGCCAAGGAGAAGGTTACAGGGGAACCACCAATACCATTTGGAATGGAATTCCGTG
  TCAGCGTTGGGATTCGCAGTACCCCCACAAGCATGACATCACTCCCGAGAACTTCAAATGCAAGGACCTT
  AGAGAAAATTATTGCCGCAATCCGGATGGGGCTGAATCACCATGGTGTTTTACCACTGATCCAAACATCC
  GAGTTGGTTACTGCTCTCAAATTCCCAAATGTGACGTGTCAAGTGGACAAGATTGTTATCGTGGCAATGG
  GAAAAACTACATGGGCAACTTATCCAAAACAAGGTCTGGACTCACATGTTCCATGTGGGACAAGAATATG
  GAGGATTTACACCGTCATATCTTCTGGGAGCCAGACGCTAGCAAGTTGACTAAGAATTACTGCCGGAACC
  CCGATGACGACGCCCATGGACCTTGGTGCTACACAGGGAATCCTCTCGTTCCTTGGGATTATTGCCCTAT
  TTCCCGTTGTGAAGGAGATACTACACCTACAATTGTCAATTTGGACCATCCTGTAATATCCTGTGCCAAA
  ACAAAACAACTGCGAGTTGTAAATGGCATTCCAACACAAACAACAGTAGGGTGGATGGTTAGTTTGAAAT
  ACAGGAATAAACACATCTGTGGGGGATCATTGATAAAGGAAAGTTGGGTTCTTACTGCAAGGCAATGTTT
  TCCAGCTAGAAACAAAGACTTGAAAGACTATGAAGCTTGGCTTGGAATCCATGATGTCCATGAGAGAGGC
  GAGGAGAAACGCAAACAGATCTTAAACATTTCCCAGCTAGTCTATGGACCTGAAGGCTCAGATTTGGTTT
  TACTGAAGCTTGCTCGCCCTGCAATCCTGGATAACTTTGTCAGTACAATTGATTTACCTAGTTATGGCTG
  TACAATCCCTGAAAAGACTACTTGCAGTATTTACGGCTGGGGCTACACTGGATTGATCAACGCAGATGGT
  TTATTACGAGTAGCTCATCTGTATATTATGGGGAATGAGAAATGCAGTCAGCACCATCAAGGCAAGGTGA
  CTTTGAATGAGTCTGAATTATGTGCTGGGGCTGAAAAGATTGGATCAGGACCTTGTGAGGGAGATTATGG
  TGGCCCACTCATTTGTGAACAACACAAAATGAGAATGGTTCTTGGTGTCATTGTTCCTGGTCGTGGATGT
  GCCATCCCAAATCGTCCTGGTATTTTTGTTCGAGTAGCATATTATGCAAAATGGATACACAAAGTAATTT
  TGACATACAAGTTGTGACATACAAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213062 representing NM_017017
  Red=Cloning site Green=Tags(s)
  
  MMWGTKLLPVLLLQHVLLHLLLLPVTIPYAEGQKKRRNTLHEFKKSAKTTLTKEDPLVKIKTKKVNSADE
  CANRCIRNKGFPFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGT
  VSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEV
  ECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTP
  WEYCAIKMCAHSAVNETDVPMETTECIKGQGEGYRGTTNTIWNGIPCQRWDSQYPHKHDITPENFKCKDL
  RENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNM
  EDLHRHIFWEPDASKLTKNYCRNPDDDAHGPWCYTGNPLVPWDYCPISRCEGDTTPTIVNLDHPVISCAK
  TKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERG
  EEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADG
  LLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGC
  AIPNRPGIFVRVAYYAKWIHKVILTYKL*HTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017017
• ORF Size: 2184 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2532 bp
• RefSeq ORF: 2187 bp
• Locus ID: 24446
• UniProt ID: P17945
• Cytogenetics: 4q12
• MW: 82.9 kDa
• Gene Summary: plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle [RGD, Feb 2006]