Cftr (NM_031506) Rat Untagged Clone

CAT#: RN210033

Cftr (untagged ORF) - Rat cystic fibrosis transmembrane conductance regulator homolog (Cftr), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cftr
• Synonyms: RGD1561193
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210033 representing NM_031506
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAGTCGCCTCTGGAGAAAGCCAGCTTTATCTCCAAACTCTTCTTCAGCTGGACCACACCAATTT
  TGAGAAAAGGGTACAGACACCACTTGGAGCTGTCAGACATATACCAAGCCCCTTCTTCTGATTCAGCTGA
  CCACCTGTCTGAAAAGCTAGAAAGAGAATGGGACAGGGAACAAGCTTCCAAAAAGAAGCCCCAGCTTATC
  CACGCCCTCCGGCGATGCTTTGTCTGGAGATTCGTCTTCTACGGAGTCTTGTTATACCTCGGGGAAGTCA
  CCAAGGCTGTCCAGCCTGTCTTGCTAGGAAGAATCATAGCATCCTATGATCCAGACAACACGGAGGAGCG
  TTCCATTGCCATTTACCTAGGCATAGGCTTATGCCTCCTCTTCATTGTCAGGACACTGCTTCTGCACCCA
  GCTATTTTTGGCCTTCATCACATTGGAATGCAGATGAGAATAGCCATGTTTAGCTTGATTTATAAGAAGA
  CTTTAAAGTTGTCAAGCCGTGTTCTTGATAAAATAAGTATTGGACAACTTATTAGTCTTCTTTCCAACAA
  CCTGAACAAATTTGATGAAGGACTTGCCTTGGCACATTTTATATGGATTGCTCCTTTACAAGTGGTTCTT
  CTGATGGGGCTTCTCTGGGACTTGTTACAGTTCTCAGCCTTCTGTGGCCTTGGTTTACTGATAGTCTTGG
  TTATTTTTCAAGCTATCTTAGGGAAGATGATGGTGAAGTACAGAGATAAAAGAGCTGCAAAGATCAATGA
  AAGACTTGTGATCACATCAGAAGTTATTGACAATATCTATTCTGTTAAGGCATATTGTTGGGAATCAGCG
  ATGGAGAAGATAATTGAAAGCTTGAGAGAGGAGGAACTGAAAATGACCCGGAGGTCAGCCTATATGAGGT
  TCTTCACTAGCTCTGCCTTCTTCTTTTCAGGGTTTTTTGTAGTCTTTTTATCTGTGCTTCCCTACACAGT
  CATCAATGGAATCGTCCTCCGAAAAATATTCACAACCATTTCATTCTGCATTGTCCTGCGTATGTCAGTC
  ACACGGCAATTCCCCACTGCTGTACAGATATGGTATGATTCTTTGGGAATGATAAGAAAAATACAGGATT
  TCCTACAGACTCAAGAATATAAAGTGCTGGAGTATAACTTAATGTTCACAGGCCTAGTCATGGAGAATGT
  AACAGCATTTTGGGAGGAGGGATTTCAGGAATTACTGGAGAAAGTACAACTAAACAATGATGACAGAAAA
  ACTTCCAATGGTGAGAACCACCTCAGTTTTAGTCATCTCTGTCTAGTGGGAAATCCTGTGTTGAAAAACA
  TCAATTTGAATATAAAGAAAGGAGAGATGTTGGCTATTACTGGATCTACTGGAGCAGGAAAGACATCACT
  CCTGATGCTGATACTGGGAGAACTGGAAGCGTCAGAGGGAATTATTAAGCACAGTGGAAGAGTTTCATTC
  TCCTCTCAAATTTCTTGGATTATGCCGGGTACTATCAAAGAAAATATCATCTTTGGTGTTTCCTATGATG
  AGTACAGATATAAGAGTGTTGTCAAAGCTTGCCAACTACAGGAGGACATCACCAAGTTTGCAGAACAAGA
  CAACACAGTTCTTGGAGAAGGTGGAGTCACACTCAGTGGAGGTCAACGTGCAAGAATTTCTTTAGCAAGA
  GCAGTATATAAAGATGCTGATTTGTACCTGTTAGATTCCCCCTTCGGATATCTAGATGTTTTAACTGAAG
  AACAAATATTTGAAAGTTGTGTCTGTAAATTGATGGCCAGCAAAACTAGGATTCTGGTCACATCTAAAAT
  GGAGCAGTTAAAGAAAGCTGACAAGATACTCATTTTGCATGAGGGCAGCAGCTATTTTTATGGGACGTTT
  TCTGAACTACAAAGTCTACGTCCAGACTTCAGTTCCAAACTCATGGGGTATGATACTTTTGACCAGTTTA
  CTGAGGAAAGAAGGAGTTCAATTCTAACTGAGACCTTACGCAGGTTCTCAGTGGACGATGCCTCTACCAC
  CTGGAACAAAGCCAAACAGTCATTTAGACAGACTGGAGAGTTTGGAGAAAAAAGGAAGAACTCTATTCTA
  AGTTCATTCAGCTCTGTGAAGAAAATTTCCATTGTGCAAAAGACTCCATTGTCCATAGAAGGAGAATCTG
  ATGACCTCCAAGAAAGGAGACTGTCCCTGGTTCCAGACTCTGAACATGGAGAGGCCGCTCTGCCTCGCAG
  CAACATGATCACCGCCGGGCCCACGTTTCCAGGCAGAAGAAGACAGTCTGTTTTGGATCTTATGACATTC
  ACACCCAGCTCAGTCTCCAGCAGTCTCCAGAGGACCAGAGCTTCTATTCGAAAAATATCCTTAGCCCCTC
  GGATAAGCTTAAAGGAAGAGGATATATATTCAAGGCGATTATCGCAAGATAGCACACTGAACATCACCGA
  AGAAATTAATGAAGAAGATTTAAAGGAGTGTTTTTTTGATGATATGGTAAAGATCCCCACGGTGACAACA
  TGGAACACATACCTTCGATATTTCACGCTCCACAGAGGCTTATTTGCAGTGCTGATTTGGTGCGTGCTGG
  TTTTTCTGGTTGAGGTGGCTGCTTCTTTATTTGTGTTGTGGTTGCTTAAAAACAACCCCGTTAATGGTGG
  AAACAATGGCACTAAAATTGCCAATACCTCCTATGTTGTGGTCATCACCAGTTCCAGCTTCTATTACATT
  TTTTACATTTACGTGGGAGTGGCTGACACTTTGCTTGCTCTGAGCCTCTTCAGAGGTTTGCCCCTGGTGC
  ATACGTTAATCACAGCATCAAAAATTTTGCACAGGAAAATGTTACATTCTATTCTTCATGCCCCTATGTC
  AACCTTCAACAAGCTGAAAGCAGGTGGGATTCTTAACAGATTCTCCAAAGATATAGCAATTTTGGATGAC
  TTTCTGCCTCTTACCATATTTGACTTCATCCAGTTGCTGTTCATTGTGGTCGGAGCAATAATAGTTGTCT
  CGGCTTTACAACCCTACATCTTCCTAGCAACGGTGCCAGGGCTAGCAGTCTTCATTTTACTGAGGGCATA
  CTTCCTTCATACCTCCCAACAGCTCAAACAACTGGAATCTGAAGGCAGGAGTCCAATTTTCACGCACCTT
  GTTACAAGCTTAAAAGGACTGTGGACACTTCGAGCCTTCCGACGCCAGACTTACTTTGAAACCCTATTCC
  ACAAAGCTCTGAATTTGCACACTGCCAACTGGTTTATGTATCTGGCAACCTTGCGCTGGTTCCAGATGAG
  AATAGACATGATTTTCGTCCTCTTCTTCATTGTTGTTACCTTCATCTCCATTTTAACAACAGGTGAAGGA
  GAAGGAACGACTGGTATTATTCTAACTTTAGCTATGAATATTATGAGTACTTTGCAATGGGCTGTGAACT
  CAAGCATTGATACAGATAGCTTGATGCGATCTGTGAGCAGAGTCTTTAAGTTTATTGATATACAAACAGA
  AGAAAGTATATGTACCAAGATAATGAAAGAACTACATAGTGAAGACTCACCTAATGCTTTAGTCATTAAG
  AATGAACATGTGAAGAAATGTGATACCTGGCCCTCTGGAGGTGAAATGGTTGTCAAAGACCTTACTGTGA
  AATATGTGGATGATGGGAATGCCATATTAGAGAACATTTCTTTTTCAATAAGTCCTGGCCAGAGGGTGGG
  GCTCTTAGGAAGAACTGGCTCAGGAAAAAGTACTTTGCTGTCAGCCTTTTTACGAATGTTGAACATTAAA
  GGCGAAATACAGATCGATGGTGTCTCCTGGAACTCAATGACCTTACAAGAATGGAGAAAAGCTTTTGGAG
  TGATAACACAGAAAGTATTTATCTTTTCTGGAACATTCAGACAAAACTTGGATCCCAATGGAAAATGGAG
  AGATGAAGAAATATGGAAAGTTGCAGATCAGGTTGGCCTCAAGTCTGTGATAGAGCAGTTTCCTGGACAG
  CTCAACTTTACCCTTGTGGATGGGGGTTATGTGCTAAGCCATGGTCACAAGCAGTTGATGTGCTTGGCCC
  GATCTGTTCTCAGTAAGGCCAAGATCATACTGCTTGATGAGCCTAGTGCCAATCTAGACCCCATAACATA
  CCAAGTGATTCGACGAGTTCTAAGACAAGCCTTCGCTGGTTGCACAGTAGTCCTCTGTGAACACAGGATA
  GAAGCAATGTTGGACTGTCAGCGATTTTTGGTCATAGAGCAGGGCAATGTCTGGCAGTATGAATCCCTCC
  AGGCACTTCTGAGCGAGAAGAGTGTCTTCCAGCGAGCCCTTAGCTCCTCAGAAAAGATGAAGCTCTTCCA
  CGGCCGGCACTCCAGCAAGCAGAAGCCTCGGACACAGATCACTGCTGTGAAAGAGGAGACGGAGGAAGAA
  GTGCAAGAGACCCGGCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031506
• Insert Size: 4431 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031506.1, NP_113694.1
• RefSeq Size: 6287 bp
• RefSeq ORF: 4431 bp
• Locus ID: 24255
• UniProt ID: P34158
• Cytogenetics: 4q21
• Gene Summary: cAMP-activated chloride channel and ATP binding cassette (ABC) transporter involved in intestinal anion secretion [RGD, Feb 2006]