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Cftr (NM_031506) Rat Tagged ORF Clone
CAT#: RR210033
- TrueORF®
Cftr (Myc-DDK-tagged ORF) - Rat cystic fibrosis transmembrane conductance regulator homolog (Cftr), (10 ug)
"NM_031506" in other vectors (1)
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Frequently bought together (3)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
USD 198.00
Other products for "Cftr"
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Cftr |
| Synonyms | RGD1561193 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210033 representing NM_031506
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAGTCGCCTCTGGAGAAAGCCAGCTTTATCTCCAAACTCTTCTTCAGCTGGACCACACCAATTT TGAGAAAAGGGTACAGACACCACTTGGAGCTGTCAGACATATACCAAGCCCCTTCTTCTGATTCAGCTGA CCACCTGTCTGAAAAGCTAGAAAGAGAATGGGACAGGGAACAAGCTTCCAAAAAGAAGCCCCAGCTTATC CACGCCCTCCGGCGATGCTTTGTCTGGAGATTCGTCTTCTACGGAGTCTTGTTATACCTCGGGGAAGTCA CCAAGGCTGTCCAGCCTGTCTTGCTAGGAAGAATCATAGCATCCTATGATCCAGACAACACGGAGGAGCG TTCCATTGCCATTTACCTAGGCATAGGCTTATGCCTCCTCTTCATTGTCAGGACACTGCTTCTGCACCCA GCTATTTTTGGCCTTCATCACATTGGAATGCAGATGAGAATAGCCATGTTTAGCTTGATTTATAAGAAGA CTTTAAAGTTGTCAAGCCGTGTTCTTGATAAAATAAGTATTGGACAACTTATTAGTCTTCTTTCCAACAA CCTGAACAAATTTGATGAAGGACTTGCCTTGGCACATTTTATATGGATTGCTCCTTTACAAGTGGTTCTT CTGATGGGGCTTCTCTGGGACTTGTTACAGTTCTCAGCCTTCTGTGGCCTTGGTTTACTGATAGTCTTGG TTATTTTTCAAGCTATCTTAGGGAAGATGATGGTGAAGTACAGAGATAAAAGAGCTGCAAAGATCAATGA AAGACTTGTGATCACATCAGAAGTTATTGACAATATCTATTCTGTTAAGGCATATTGTTGGGAATCAGCG ATGGAGAAGATAATTGAAAGCTTGAGAGAGGAGGAACTGAAAATGACCCGGAGGTCAGCCTATATGAGGT TCTTCACTAGCTCTGCCTTCTTCTTTTCAGGGTTTTTTGTAGTCTTTTTATCTGTGCTTCCCTACACAGT CATCAATGGAATCGTCCTCCGAAAAATATTCACAACCATTTCATTCTGCATTGTCCTGCGTATGTCAGTC ACACGGCAATTCCCCACTGCTGTACAGATATGGTATGATTCTTTGGGAATGATAAGAAAAATACAGGATT TCCTACAGACTCAAGAATATAAAGTGCTGGAGTATAACTTAATGTTCACAGGCCTAGTCATGGAGAATGT AACAGCATTTTGGGAGGAGGGATTTCAGGAATTACTGGAGAAAGTACAACTAAACAATGATGACAGAAAA ACTTCCAATGGTGAGAACCACCTCAGTTTTAGTCATCTCTGTCTAGTGGGAAATCCTGTGTTGAAAAACA TCAATTTGAATATAAAGAAAGGAGAGATGTTGGCTATTACTGGATCTACTGGAGCAGGAAAGACATCACT CCTGATGCTGATACTGGGAGAACTGGAAGCGTCAGAGGGAATTATTAAGCACAGTGGAAGAGTTTCATTC TCCTCTCAAATTTCTTGGATTATGCCGGGTACTATCAAAGAAAATATCATCTTTGGTGTTTCCTATGATG AGTACAGATATAAGAGTGTTGTCAAAGCTTGCCAACTACAGGAGGACATCACCAAGTTTGCAGAACAAGA CAACACAGTTCTTGGAGAAGGTGGAGTCACACTCAGTGGAGGTCAACGTGCAAGAATTTCTTTAGCAAGA GCAGTATATAAAGATGCTGATTTGTACCTGTTAGATTCCCCCTTCGGATATCTAGATGTTTTAACTGAAG AACAAATATTTGAAAGTTGTGTCTGTAAATTGATGGCCAGCAAAACTAGGATTCTGGTCACATCTAAAAT GGAGCAGTTAAAGAAAGCTGACAAGATACTCATTTTGCATGAGGGCAGCAGCTATTTTTATGGGACGTTT TCTGAACTACAAAGTCTACGTCCAGACTTCAGTTCCAAACTCATGGGGTATGATACTTTTGACCAGTTTA CTGAGGAAAGAAGGAGTTCAATTCTAACTGAGACCTTACGCAGGTTCTCAGTGGACGATGCCTCTACCAC CTGGAACAAAGCCAAACAGTCATTTAGACAGACTGGAGAGTTTGGAGAAAAAAGGAAGAACTCTATTCTA AGTTCATTCAGCTCTGTGAAGAAAATTTCCATTGTGCAAAAGACTCCATTGTCCATAGAAGGAGAATCTG ATGACCTCCAAGAAAGGAGACTGTCCCTGGTTCCAGACTCTGAACATGGAGAGGCCGCTCTGCCTCGCAG CAACATGATCACCGCCGGGCCCACGTTTCCAGGCAGAAGAAGACAGTCTGTTTTGGATCTTATGACATTC ACACCCAGCTCAGTCTCCAGCAGTCTCCAGAGGACCAGAGCTTCTATTCGAAAAATATCCTTAGCCCCTC GGATAAGCTTAAAGGAAGAGGATATATATTCAAGGCGATTATCGCAAGATAGCACACTGAACATCACCGA AGAAATTAATGAAGAAGATTTAAAGGAGTGTTTTTTTGATGATATGGTAAAGATCCCCACGGTGACAACA TGGAACACATACCTTCGATATTTCACGCTCCACAGAGGCTTATTTGCAGTGCTGATTTGGTGCGTGCTGG TTTTTCTGGTTGAGGTGGCTGCTTCTTTATTTGTGTTGTGGTTGCTTAAAAACAACCCCGTTAATGGTGG AAACAATGGCACTAAAATTGCCAATACCTCCTATGTTGTGGTCATCACCAGTTCCAGCTTCTATTACATT TTTTACATTTACGTGGGAGTGGCTGACACTTTGCTTGCTCTGAGCCTCTTCAGAGGTTTGCCCCTGGTGC ATACGTTAATCACAGCATCAAAAATTTTGCACAGGAAAATGTTACATTCTATTCTTCATGCCCCTATGTC AACCTTCAACAAGCTGAAAGCAGGTGGGATTCTTAACAGATTCTCCAAAGATATAGCAATTTTGGATGAC TTTCTGCCTCTTACCATATTTGACTTCATCCAGTTGCTGTTCATTGTGGTCGGAGCAATAATAGTTGTCT CGGCTTTACAACCCTACATCTTCCTAGCAACGGTGCCAGGGCTAGCAGTCTTCATTTTACTGAGGGCATA CTTCCTTCATACCTCCCAACAGCTCAAACAACTGGAATCTGAAGGCAGGAGTCCAATTTTCACGCACCTT GTTACAAGCTTAAAAGGACTGTGGACACTTCGAGCCTTCCGACGCCAGACTTACTTTGAAACCCTATTCC ACAAAGCTCTGAATTTGCACACTGCCAACTGGTTTATGTATCTGGCAACCTTGCGCTGGTTCCAGATGAG AATAGACATGATTTTCGTCCTCTTCTTCATTGTTGTTACCTTCATCTCCATTTTAACAACAGGTGAAGGA GAAGGAACGACTGGTATTATTCTAACTTTAGCTATGAATATTATGAGTACTTTGCAATGGGCTGTGAACT CAAGCATTGATACAGATAGCTTGATGCGATCTGTGAGCAGAGTCTTTAAGTTTATTGATATACAAACAGA AGAAAGTATATGTACCAAGATAATGAAAGAACTACATAGTGAAGACTCACCTAATGCTTTAGTCATTAAG AATGAACATGTGAAGAAATGTGATACCTGGCCCTCTGGAGGTGAAATGGTTGTCAAAGACCTTACTGTGA AATATGTGGATGATGGGAATGCCATATTAGAGAACATTTCTTTTTCAATAAGTCCTGGCCAGAGGGTGGG GCTCTTAGGAAGAACTGGCTCAGGAAAAAGTACTTTGCTGTCAGCCTTTTTACGAATGTTGAACATTAAA GGCGAAATACAGATCGATGGTGTCTCCTGGAACTCAATGACCTTACAAGAATGGAGAAAAGCTTTTGGAG TGATAACACAGAAAGTATTTATCTTTTCTGGAACATTCAGACAAAACTTGGATCCCAATGGAAAATGGAG AGATGAAGAAATATGGAAAGTTGCAGATCAGGTTGGCCTCAAGTCTGTGATAGAGCAGTTTCCTGGACAG CTCAACTTTACCCTTGTGGATGGGGGTTATGTGCTAAGCCATGGTCACAAGCAGTTGATGTGCTTGGCCC GATCTGTTCTCAGTAAGGCCAAGATCATACTGCTTGATGAGCCTAGTGCCAATCTAGACCCCATAACATA CCAAGTGATTCGACGAGTTCTAAGACAAGCCTTCGCTGGTTGCACAGTAGTCCTCTGTGAACACAGGATA GAAGCAATGTTGGACTGTCAGCGATTTTTGGTCATAGAGCAGGGCAATGTCTGGCAGTATGAATCCCTCC AGGCACTTCTGAGCGAGAAGAGTGTCTTCCAGCGAGCCCTTAGCTCCTCAGAAAAGATGAAGCTCTTCCA CGGCCGGCACTCCAGCAAGCAGAAGCCTCGGACACAGATCACTGCTGTGAAAGAGGAGACGGAGGAAGAA GTGCAAGAGACCCGGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210033 representing NM_031506
Red=Cloning site Green=Tags(s) MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSSDSADHLSEKLEREWDREQASKKKPQLI HALRRCFVWRFVFYGVLLYLGEVTKAVQPVLLGRIIASYDPDNTEERSIAIYLGIGLCLLFIVRTLLLHP AIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLISLLSNNLNKFDEGLALAHFIWIAPLQVVL LMGLLWDLLQFSAFCGLGLLIVLVIFQAILGKMMVKYRDKRAAKINERLVITSEVIDNIYSVKAYCWESA MEKIIESLREEELKMTRRSAYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSV TRQFPTAVQIWYDSLGMIRKIQDFLQTQEYKVLEYNLMFTGLVMENVTAFWEEGFQELLEKVQLNNDDRK TSNGENHLSFSHLCLVGNPVLKNINLNIKKGEMLAITGSTGAGKTSLLMLILGELEASEGIIKHSGRVSF SSQISWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQEDITKFAEQDNTVLGEGGVTLSGGQRARISLAR AVYKDADLYLLDSPFGYLDVLTEEQIFESCVCKLMASKTRILVTSKMEQLKKADKILILHEGSSYFYGTF SELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDASTTWNKAKQSFRQTGEFGEKRKNSIL SSFSSVKKISIVQKTPLSIEGESDDLQERRLSLVPDSEHGEAALPRSNMITAGPTFPGRRRQSVLDLMTF TPSSVSSSLQRTRASIRKISLAPRISLKEEDIYSRRLSQDSTLNITEEINEEDLKECFFDDMVKIPTVTT WNTYLRYFTLHRGLFAVLIWCVLVFLVEVAASLFVLWLLKNNPVNGGNNGTKIANTSYVVVITSSSFYYI FYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTFNKLKAGGILNRFSKDIAILDD FLPLTIFDFIQLLFIVVGAIIVVSALQPYIFLATVPGLAVFILLRAYFLHTSQQLKQLESEGRSPIFTHL VTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEG EGTTGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRVFKFIDIQTEESICTKIMKELHSEDSPNALVIK NEHVKKCDTWPSGGEMVVKDLTVKYVDDGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIK GEIQIDGVSWNSMTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWRDEEIWKVADQVGLKSVIEQFPGQ LNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSANLDPITYQVIRRVLRQAFAGCTVVLCEHRI EAMLDCQRFLVIEQGNVWQYESLQALLSEKSVFQRALSSSEKMKLFHGRHSSKQKPRTQITAVKEETEEE VQETRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031506 |
| ORF Size | 4428 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031506.1, NP_113694.1 |
| RefSeq Size | 6287 bp |
| RefSeq ORF | 4431 bp |
| Locus ID | 24255 |
| UniProt ID | P34158 |
| Cytogenetics | 4q21 |
| MW | 167.8 kDa |
| Gene Summary | cAMP-activated chloride channel and ATP binding cassette (ABC) transporter involved in intestinal anion secretion [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210033 | Cftr (untagged ORF) - Rat cystic fibrosis transmembrane conductance regulator homolog (Cftr), (10 ug) |
USD 2,021.00 |
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