Flt1 (NM_019306) Rat Untagged Clone

CAT#: RN201567

Flt1 (untagged ORF) - Rat fms-related tyrosine kinase 1 (Flt1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Flt1
• Synonyms: FLT-1; VEGFR-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201567 representing NM_019306
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCTGCTGGGACACCGCGGTCCTGCCTTGCGCGCTGCTCGGGTGTCTGCTTCTCACAGGATATT
  GTTCAGGGTCGAAGTTAAAAGGACCAGAACTGAGTTTAAAAGGCACCCAGCACGTCATGCAAGCGGGCCA
  GACTCTCTTTCTCAAGTGCAGAGGGGAGGCAGCCCACTCCTGGTCTCTGCCTACTACAGTGAGCCAGGAG
  GACAAAAAGCTGAGCGTCACTAGATCAGCCTGTGGAAGGAATAACAGACAATTCTGCAGCACCTTGACCT
  TGAACATGGCGCAGGCCAACCACACGGGCCTCTACTCCTGCAGATACCTGCCTAAATCCACCTCGAAGGA
  AAAGAAAATGGAATCTGCAATCTACATATTTGTTAGTGATGCAGGGAGTCCTTTCATAGAGATGCACAGT
  GACATACCCAAACTCGTGCACATGACAGAAGGAAGAGAGCTCATCATCCCCTGCCGGGTGACGTCTCCCA
  ACATCACAGTCACTCTAAAAAAGTTTCCATTTGACGCTCTTACCCCTGACGGGCAAAGAATAGCGTGGGA
  CAGTAGGAGAGGCTTTATAATAGCAAATGCAACGTACAAAGAGATAGGACTGCTGACCTGCGAAGCCACA
  GTTAACGGGCACCTGTACCAGACAAGTTATCTGACCCATCGGCAGACCAATACAATCCTAGATGTCCAAA
  TAAGCCCACCGAGCCCCGTGAGATTCCTCCGTGGTCAAACTCTTGTCCTCAACTGCACCGTCACCACGGA
  CCTCAATACAAGGGTGCAGATGAGCTGGAATTACCCTGGTAAAGCAACTAAGAGAGCATCTATCAGGCAG
  CGGATTGACCAAAGCAATCCCCACAGCAATGTGTTCCACAGCGTTCTTAAGATCAACAACGTGGAGAGCC
  GGGACAAGGGACTCTACACTTGTCGTGTGAAGAGTGGGTCGTCATTCCGGACTTTCAACACCTCTGTGCA
  TGTGTATGAAAAGGGATTTATCAGCGTGAAGCATCGGAAGCAACAGGTGCAGGAAACCATAGCAGGAAAA
  CGGTCCCATCGGCTGTCCATGAAAGTGAAGGCCTTCCCCTCGCCAGAAGTCGTATGGTTAAAAGATGGCG
  TACCCGCAACGGAGAAATCTGCTCGCTATTCGGTGCATGGCTACTCGTTAATTATCAAAGATGTAACTGC
  CGAGGACGCAGGGGACTATACAATCTTGCTGGGCATAAAACAGTCAAAGCTATTTAGAAACCTCACAGCC
  ACTCTCATCGTAAATGTGAAACCTCAGATCTACGAAAAGTCCGTGTCGTCCCTTCCAAGCCCACCTCTCT
  ACCCACTGGGCAGCAGACAAGTCCTCACTTGCACCGTGTATGGCATCCCTCAGCCTACCATCAAGTGGCT
  GTGGCACCCCTGTCACTACAACCACTCCAAAGAAAGGAATGACTTCTGCTTTGGGAGTGAAGAATCCTTC
  ATCCTGGATTCCAGCAGCAACATAGGAAACAGAATCGAGGGCATCACTCAGCGCATGATGGTCATAGAAG
  GAACCAATAAGACGGTTAGCACATTGGTGGTGGCTGACTCTCGGACCCCTGGAATCTACAGCTGCAAGGC
  CTTCAATAAAATAGGGACTGTGGAGAGAGACATAAGGTTTTACGTCACAGATGTGCCAAACGGCTTTCAC
  GTTTCCTTGGAAAAGATACCAACCGAAGGAGAGGACCTGAAACTGTCCTGTGTGGTCAGTAAATTCCTGT
  ACAGAGACATTACCTGGATCCTGCTACGGACAGTTAACAACAGGACCATGCACCATAGCATCAGTAAGCA
  AAAAATGGCCACCACTCAGGACTACTCCATCACTCTGAACCTTGTCATCAAGAATGTGTCTCTGGAAGAC
  TCGGGCACCTATGCCTGCAGAGCCAGGAACATATACACAGGGGAAGAGATCCTTCGGAAGACAGAAGTTC
  TCGTTAGAGATTTGGAAGCGCCACTCCTGCTTCAAAACCTCAGTGACCACGAGGTGTCCATCAGTGGCTC
  CACGACCTTAGACTGTCAAGCTAGAGGTGTCCCTGCGCCTCAGATCACTTGGTTCAAAAACAACCACAAA
  ATACAACAAGAACCGGGAATTATTTTAGGACCAGGAAACAGCACGCTGTTTATTGAAAGAGTCACAGAAG
  AGGATGAGGGTGTCTATAGGTGCCGAGCCACCAACCAGAAGGGGGTCGTGGAAAGCTCAGCGTACCTCAC
  CGTGCAAGGAACCTCAGACAAGTCAAACCTGGAGCTGATCACCCTCACGTGCACGTGTGTGGCTGCGACA
  CTCTTTTGGCTCCTTCTAACTCTCTTCATCCGAAAACTGAAGCGGTCTTCTTCCGAAGTAAAGACGGACT
  ACCTGTCAATCATCATGGACCCAGATGAAGTCCCCCTGGATGAGCAGTGTGAACGGCTGCCCTATGATGC
  CAGCAAGTGGGAGTTTGCGCGGGAGAGACTTAAACTAGGCAAATCACTCGGGAGAGGGGCTTTTGGGAAG
  GTGGTTCAGGCCTCGGCATTTGGCATTAAGAAATCACCCACCTGCCGGACTGTGGCTGTGAAGATGTTGA
  AAGAGGGGGCCACAGCCAGTGAGTACAAAGCTCTGATGACCGAACTCAAGATCTTGACCCACATCGGCCA
  TCATCTGAATGTGGTTAACCTCCTGGGAGCCTGCACCAAGCAAGGAGGGCCTCTGATGGTGATCGTGGAA
  TACTGCAAATATGGAAACCTGTCCAACTACCTTAAGAGCAAACGTGACTTCTTCTGTCTCAACAAGGATG
  CAGCCTTGCATATGGAGCCCAAGAAAGAAAAGCTGGAGCCAGACCTGGAGCAGGACCAGAAACCCCGCCT
  AGACAGTGTCAGCAGCAGCGAGAGTTTCACCAGCTCCGGCTTCCAGGAAGATAAGAGTGTGAGCGATGTG
  GAAGGAGGCGAGGATTACAGTGAGATCTCCAAGCAGCCCCTCACCATGGAAGACCTGATCTCCTACAGTT
  TCCAAGTGGCCAGAGGCATGGAGTTTCTGTCCTCCAGAAAGTGCATTCATCGCGACCTGGCAGCACGGAA
  CATCCTTTTATCTGAGAACAATGTTGTGAAGATTTGCGACTTTGGCCTGGCCCGGGATATTTATAAGAAC
  CCTGATTACGTGAGGAGAGGAGATACTCGACTTCCCCTAAAATGGATGGCTCCCGAATCCATCTTTGACA
  AGGTCTACAGCACCAAGAGTGACGTGTGGTCCTACGGAGTGTTGCTGTGGGAGATCTTTTCCTTAGGGGG
  TTCTCCATACCCAGGAGTGCAAATGGATGAAGACTTCTGTAGCCGGCTGAAGGAAGGCATGCGCATGAGA
  ACACCAGAGTATGCCACACCTGAAATCTACCAAATCATGCTGGATTGCTGGCACAAAGACCCCAAAGAAA
  GGCCCCGGTTTGCTGAACTTGTGGAGAAGCTCGGTGACCTGCTTCAAGCCAATGTCCAACAGGATGGTAA
  AGACTACATCCCCCTCAACGCCATACTGACTAGAAACAGTGGCTTCACATACTCAGTCCCCACCTTCTCA
  GAGGACTTTTTCAAGGATGGTTTCACGGATCCAAAGTTTCATTCTGGAAGCTCTGATGATGTGAGATACG
  TAAACGCTTTCAAATTCATGAGCCTGGAAAGAATCAAAACCTTTGAGGAGCTTTCACCAAATGCCACCTC
  CATGTTTGAAGACTATCATCTGGATACCAGCAGTCTGCTGACCTCCCCCTTGCTGAAGCGATTCACCTGG
  ACTGAGACCAAGCCCAAGGCCTCAATGAAAATAGACCTGAGAATTACTAGTAAAAGCAAGGAGGCGGGGC
  TTTCCGATCTGCCCGGACCCAGCTTCTGCTTTTCCAGCTGTGGCCACATCAGGCCTGTGCGCCAGGAGGA
  TGAAGACGACCCTGAGCTGGGGAAGGAATCCTGCTGCTCTCCGCCCCCAGACTACAACTCCGTGGTGTTG
  TACTCCTCCCCGCCTGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019306
• Insert Size: 4011 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019306.2, NP_062179.2
• RefSeq Size: 6848 bp
• RefSeq ORF: 4011 bp
• Locus ID: 54251
• Cytogenetics: 12p11
• Gene Summary: tyrosine kinase receptor for vascular endothelial growth factor; may play a role in cell proliferation and cell survival [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.