Flt1 (NM_019306) Rat Tagged ORF Clone

CAT#: RR201567

Flt1 (Myc-DDK-tagged ORF) - Rat fms-related tyrosine kinase 1 (Flt1), (10 ug)

Product Images

Circular map for RR201567

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Flt1
• Synonyms: FLT-1; VEGFR-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201567 representing NM_019306
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCTGCTGGGACACCGCGGTCCTGCCTTGCGCGCTGCTCGGGTGTCTGCTTCTCACAGGATATT
  GTTCAGGGTCGAAGTTAAAAGGACCAGAACTGAGTTTAAAAGGCACCCAGCACGTCATGCAAGCGGGCCA
  GACTCTCTTTCTCAAGTGCAGAGGGGAGGCAGCCCACTCCTGGTCTCTGCCTACTACAGTGAGCCAGGAG
  GACAAAAAGCTGAGCGTCACTAGATCAGCCTGTGGAAGGAATAACAGACAATTCTGCAGCACCTTGACCT
  TGAACATGGCGCAGGCCAACCACACGGGCCTCTACTCCTGCAGATACCTGCCTAAATCCACCTCGAAGGA
  AAAGAAAATGGAATCTGCAATCTACATATTTGTTAGTGATGCAGGGAGTCCTTTCATAGAGATGCACAGT
  GACATACCCAAACTCGTGCACATGACAGAAGGAAGAGAGCTCATCATCCCCTGCCGGGTGACGTCTCCCA
  ACATCACAGTCACTCTAAAAAAGTTTCCATTTGACGCTCTTACCCCTGACGGGCAAAGAATAGCGTGGGA
  CAGTAGGAGAGGCTTTATAATAGCAAATGCAACGTACAAAGAGATAGGACTGCTGACCTGCGAAGCCACA
  GTTAACGGGCACCTGTACCAGACAAGTTATCTGACCCATCGGCAGACCAATACAATCCTAGATGTCCAAA
  TAAGCCCACCGAGCCCCGTGAGATTCCTCCGTGGTCAAACTCTTGTCCTCAACTGCACCGTCACCACGGA
  CCTCAATACAAGGGTGCAGATGAGCTGGAATTACCCTGGTAAAGCAACTAAGAGAGCATCTATCAGGCAG
  CGGATTGACCAAAGCAATCCCCACAGCAATGTGTTCCACAGCGTTCTTAAGATCAACAACGTGGAGAGCC
  GGGACAAGGGACTCTACACTTGTCGTGTGAAGAGTGGGTCGTCATTCCGGACTTTCAACACCTCTGTGCA
  TGTGTATGAAAAGGGATTTATCAGCGTGAAGCATCGGAAGCAACAGGTGCAGGAAACCATAGCAGGAAAA
  CGGTCCCATCGGCTGTCCATGAAAGTGAAGGCCTTCCCCTCGCCAGAAGTCGTATGGTTAAAAGATGGCG
  TACCCGCAACGGAGAAATCTGCTCGCTATTCGGTGCATGGCTACTCGTTAATTATCAAAGATGTAACTGC
  CGAGGACGCAGGGGACTATACAATCTTGCTGGGCATAAAACAGTCAAAGCTATTTAGAAACCTCACAGCC
  ACTCTCATCGTAAATGTGAAACCTCAGATCTACGAAAAGTCCGTGTCGTCCCTTCCAAGCCCACCTCTCT
  ACCCACTGGGCAGCAGACAAGTCCTCACTTGCACCGTGTATGGCATCCCTCAGCCTACCATCAAGTGGCT
  GTGGCACCCCTGTCACTACAACCACTCCAAAGAAAGGAATGACTTCTGCTTTGGGAGTGAAGAATCCTTC
  ATCCTGGATTCCAGCAGCAACATAGGAAACAGAATCGAGGGCATCACTCAGCGCATGATGGTCATAGAAG
  GAACCAATAAGACGGTTAGCACATTGGTGGTGGCTGACTCTCGGACCCCTGGAATCTACAGCTGCAAGGC
  CTTCAATAAAATAGGGACTGTGGAGAGAGACATAAGGTTTTACGTCACAGATGTGCCAAACGGCTTTCAC
  GTTTCCTTGGAAAAGATACCAACCGAAGGAGAGGACCTGAAACTGTCCTGTGTGGTCAGTAAATTCCTGT
  ACAGAGACATTACCTGGATCCTGCTACGGACAGTTAACAACAGGACCATGCACCATAGCATCAGTAAGCA
  AAAAATGGCCACCACTCAGGACTACTCCATCACTCTGAACCTTGTCATCAAGAATGTGTCTCTGGAAGAC
  TCGGGCACCTATGCCTGCAGAGCCAGGAACATATACACAGGGGAAGAGATCCTTCGGAAGACAGAAGTTC
  TCGTTAGAGATTTGGAAGCGCCACTCCTGCTTCAAAACCTCAGTGACCACGAGGTGTCCATCAGTGGCTC
  CACGACCTTAGACTGTCAAGCTAGAGGTGTCCCTGCGCCTCAGATCACTTGGTTCAAAAACAACCACAAA
  ATACAACAAGAACCGGGAATTATTTTAGGACCAGGAAACAGCACGCTGTTTATTGAAAGAGTCACAGAAG
  AGGATGAGGGTGTCTATAGGTGCCGAGCCACCAACCAGAAGGGGGTCGTGGAAAGCTCAGCGTACCTCAC
  CGTGCAAGGAACCTCAGACAAGTCAAACCTGGAGCTGATCACCCTCACGTGCACGTGTGTGGCTGCGACA
  CTCTTTTGGCTCCTTCTAACTCTCTTCATCCGAAAACTGAAGCGGTCTTCTTCCGAAGTAAAGACGGACT
  ACCTGTCAATCATCATGGACCCAGATGAAGTCCCCCTGGATGAGCAGTGTGAACGGCTGCCCTATGATGC
  CAGCAAGTGGGAGTTTGCGCGGGAGAGACTTAAACTAGGCAAATCACTCGGGAGAGGGGCTTTTGGGAAG
  GTGGTTCAGGCCTCGGCATTTGGCATTAAGAAATCACCCACCTGCCGGACTGTGGCTGTGAAGATGTTGA
  AAGAGGGGGCCACAGCCAGTGAGTACAAAGCTCTGATGACCGAACTCAAGATCTTGACCCACATCGGCCA
  TCATCTGAATGTGGTTAACCTCCTGGGAGCCTGCACCAAGCAAGGAGGGCCTCTGATGGTGATCGTGGAA
  TACTGCAAATATGGAAACCTGTCCAACTACCTTAAGAGCAAACGTGACTTCTTCTGTCTCAACAAGGATG
  CAGCCTTGCATATGGAGCCCAAGAAAGAAAAGCTGGAGCCAGACCTGGAGCAGGACCAGAAACCCCGCCT
  AGACAGTGTCAGCAGCAGCGAGAGTTTCACCAGCTCCGGCTTCCAGGAAGATAAGAGTGTGAGCGATGTG
  GAAGGAGGCGAGGATTACAGTGAGATCTCCAAGCAGCCCCTCACCATGGAAGACCTGATCTCCTACAGTT
  TCCAAGTGGCCAGAGGCATGGAGTTTCTGTCCTCCAGAAAGTGCATTCATCGCGACCTGGCAGCACGGAA
  CATCCTTTTATCTGAGAACAATGTTGTGAAGATTTGCGACTTTGGCCTGGCCCGGGATATTTATAAGAAC
  CCTGATTACGTGAGGAGAGGAGATACTCGACTTCCCCTAAAATGGATGGCTCCCGAATCCATCTTTGACA
  AGGTCTACAGCACCAAGAGTGACGTGTGGTCCTACGGAGTGTTGCTGTGGGAGATCTTTTCCTTAGGGGG
  TTCTCCATACCCAGGAGTGCAAATGGATGAAGACTTCTGTAGCCGGCTGAAGGAAGGCATGCGCATGAGA
  ACACCAGAGTATGCCACACCTGAAATCTACCAAATCATGCTGGATTGCTGGCACAAAGACCCCAAAGAAA
  GGCCCCGGTTTGCTGAACTTGTGGAGAAGCTCGGTGACCTGCTTCAAGCCAATGTCCAACAGGATGGTAA
  AGACTACATCCCCCTCAACGCCATACTGACTAGAAACAGTGGCTTCACATACTCAGTCCCCACCTTCTCA
  GAGGACTTTTTCAAGGATGGTTTCACGGATCCAAAGTTTCATTCTGGAAGCTCTGATGATGTGAGATACG
  TAAACGCTTTCAAATTCATGAGCCTGGAAAGAATCAAAACCTTTGAGGAGCTTTCACCAAATGCCACCTC
  CATGTTTGAAGACTATCATCTGGATACCAGCAGTCTGCTGACCTCCCCCTTGCTGAAGCGATTCACCTGG
  ACTGAGACCAAGCCCAAGGCCTCAATGAAAATAGACCTGAGAATTACTAGTAAAAGCAAGGAGGCGGGGC
  TTTCCGATCTGCCCGGACCCAGCTTCTGCTTTTCCAGCTGTGGCCACATCAGGCCTGTGCGCCAGGAGGA
  TGAAGACGACCCTGAGCTGGGGAAGGAATCCTGCTGCTCTCCGCCCCCAGACTACAACTCCGTGGTGTTG
  TACTCCTCCCCGCCTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201567 representing NM_019306
  Red=Cloning site Green=Tags(s)
  
  MVSCWDTAVLPCALLGCLLLTGYCSGSKLKGPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQE
  DKKLSVTRSACGRNNRQFCSTLTLNMAQANHTGLYSCRYLPKSTSKEKKMESAIYIFVSDAGSPFIEMHS
  DIPKLVHMTEGRELIIPCRVTSPNITVTLKKFPFDALTPDGQRIAWDSRRGFIIANATYKEIGLLTCEAT
  VNGHLYQTSYLTHRQTNTILDVQISPPSPVRFLRGQTLVLNCTVTTDLNTRVQMSWNYPGKATKRASIRQ
  RIDQSNPHSNVFHSVLKINNVESRDKGLYTCRVKSGSSFRTFNTSVHVYEKGFISVKHRKQQVQETIAGK
  RSHRLSMKVKAFPSPEVVWLKDGVPATEKSARYSVHGYSLIIKDVTAEDAGDYTILLGIKQSKLFRNLTA
  TLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPQPTIKWLWHPCHYNHSKERNDFCFGSEESF
  ILDSSSNIGNRIEGITQRMMVIEGTNKTVSTLVVADSRTPGIYSCKAFNKIGTVERDIRFYVTDVPNGFH
  VSLEKIPTEGEDLKLSCVVSKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLED
  SGTYACRARNIYTGEEILRKTEVLVRDLEAPLLLQNLSDHEVSISGSTTLDCQARGVPAPQITWFKNNHK
  IQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGVVESSAYLTVQGTSDKSNLELITLTCTCVAAT
  LFWLLLTLFIRKLKRSSSEVKTDYLSIIMDPDEVPLDEQCERLPYDASKWEFARERLKLGKSLGRGAFGK
  VVQASAFGIKKSPTCRTVAVKMLKEGATASEYKALMTELKILTHIGHHLNVVNLLGACTKQGGPLMVIVE
  YCKYGNLSNYLKSKRDFFCLNKDAALHMEPKKEKLEPDLEQDQKPRLDSVSSSESFTSSGFQEDKSVSDV
  EGGEDYSEISKQPLTMEDLISYSFQVARGMEFLSSRKCIHRDLAARNILLSENNVVKICDFGLARDIYKN
  PDYVRRGDTRLPLKWMAPESIFDKVYSTKSDVWSYGVLLWEIFSLGGSPYPGVQMDEDFCSRLKEGMRMR
  TPEYATPEIYQIMLDCWHKDPKERPRFAELVEKLGDLLQANVQQDGKDYIPLNAILTRNSGFTYSVPTFS
  EDFFKDGFTDPKFHSGSSDDVRYVNAFKFMSLERIKTFEELSPNATSMFEDYHLDTSSLLTSPLLKRFTW
  TETKPKASMKIDLRITSKSKEAGLSDLPGPSFCFSSCGHIRPVRQEDEDDPELGKESCCSPPPDYNSVVL
  YSSPPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019306
• ORF Size: 4008 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019306.2, NP_062179.2
• RefSeq Size: 6848 bp
• RefSeq ORF: 4011 bp
• Locus ID: 54251
• Cytogenetics: 12p11
• MW: 150.3 kDa
• Gene Summary: tyrosine kinase receptor for vascular endothelial growth factor; may play a role in cell proliferation and cell survival [RGD, Feb 2006]