MOV10 (NM_001286072) Human Tagged ORF Clone

CAT#: RG239812

MOV10 (tGFP-tagged) - Human Mov10 RISC complex RNA helicase (MOV10), transcript variant 3

Product Images

Circular map for RG239812

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MOV10
• Synonyms: fSAP113; gb110
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239812 representing NM_001286072.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGTATGGAATGAAGATTGCAAATCTGGCCTACGTCACCAAGACTCGGGTCAGGTTCTTCAGACTC
  GACCGCTGGGCCGACGTGCGGTTCCCAGAAAAGAGGAGAATGAAGCTGGGGTCAGATATCAGCAAACAC
  CACAAGTCACTGCTAGCCAAGATCTTTTATGACAGGGCTGAGTATCTTCATGGGAAACATGGTGTGGAT
  GTGGAAGTCCAGGGGCCCCATGAAGCCCGAGATGGGCAGCTCCTTATCCGCCTGGATTTGAACCGCAAA
  GAGGTGCTGACCCTGAGGCTTCGGAATGGCGGAACCCAGTCTGTTACCCTCACTCACCTCTTCCCACTC
  TGCCGGACACCCCAGTTTGCTTTCTACAATGAAGACCAGGAGTTGCCCTGTCCACTGGGCCCCGGTGAA
  TGCTATGAACTCCATGTCCATTGTAAGACCAGCTTTGTGGGCTACTTCCCAGCCACAGTGCTCTGGGAG
  CTGCTGGGACCTGGGGAGTCGGGTTCAGAAGGAGCCGGCACATTCTACATTGCCCGCTTCTTGGCTGCC
  GTCGCCCACAGCCCCCTGGCTGCACAGCTGAAGCCCATGACTCCCTTCAAGCGGACCCGGATCACCGGA
  AACCCTGTGGTGACCAATCGGATAGAGGAAGGAGAGAGACCTGACCGCGCTAAGGGCTATGACCTGGAG
  TTAAGTATGGCGCTGGGGACATACTACCCACCTCCCCGCCTCAGGCAGCTGCTCCCCATGCTTCTTCAG
  GGAACAAGTATCTTCACTGCCCCTAAGGAGATCGCAGAGATCAAGGCCCAGCTGGAGACAGCCCTGAAG
  TGGAGGAACTATGAGGTGAAGCTGCGGCTGCTGCTGCACCTGGAGGAACTGCAGATGGAGCATGATATC
  CGGCACTATGACCTGGAGTCGGTGCCCATGACCTGGGACCCTGTGGACCAGAACCCCAGGCTGCTCACG
  CTGGAGGTTCCTGGAGTGACTGAGAGCCGCCCCTCAGTGCTACGGGGCGACCACCTGTTTGCCCTTTTG
  TCCTCGGAGACACACCAGGAGGACCCCATCACATATAAGGGCTTTGTGCACAAGGTGGAATTGGACCGT
  GTCAAGCTGAGCTTTTCCATGAGCCTCCTGAGCCGCTTTGTGGATGGGCTGACCTTCAAGGTGAACTTT
  ACCTTCAACCGCCAGCCGCTGCGAGTCCAGCACCGTGCCCTGGAGCTGACAGGGCGCTGGCTGCTGTGG
  CCCATGCTCTTTCCTGTGGCACCTCGGGACGTCCCGCTGCTGCCCTCAGATGTGAAACTCAAGCTGTAC
  GACCGGAGTCTGGAGTCAAACCCAGAGCAGCTGCAGGCCATGAGGCACATTGTTACGGGCACCACCCGT
  CCAGCCCCCTACATCATCTTTGGGCCTCCAGGCACCGGCAAGACTGTCACGTTAGTGGAGGCAATTAAG
  CAGGTGGTGAAGCACTTGCCCAAAGCCCACATCTTGGCCTGCGCTCCATCCAACTCAGGGGCTGACCTA
  CTCTGTCAAAGGCTCCGGGTCCACCTTCCTAGCTCCATCTACCGCCTCCTGGCCCCCAGCAGGGACATC
  CGCATGGTACCTGAGGACATCAAGCCCTGCTGCAACTGGGACGCAAAGAAGGGGGAGTATGTATTTCCC
  GCCAAGAAGAAGCTGCAGGAATACCGGGTCTTAATTACCACCCTCATCACTGCCGGCAGGTTGGTCTCG
  GCCCAGTTTCCCATTGATCACTTCACACACATCTTCATCGATGAGGCTGGCCACTGCATGGAGCCTGAG
  AGTCTGGTAGCTATAGCAGGGCTGATGGAAGTAAAGGAAACAGGTGATCCAGGAGGGCAGCTGGTGCTG
  GCAGGAGACCCTCGGCAGCTGGGGCCTGTGCTGCGTTCCCCACTGACCCAGAAGCATGGACTGGGATAC
  TCACTGCTGGAGCGGCTGCTCACCTACAACTCCCTGTACAAGAAGGGCCCTGATGGCTATGACCCCCAG
  TTCATAACCAAGCTGCTCCGCAACTACAGGTCTCATCCCACCATCCTGGACATTCCTAACCAGCTCTAT
  TATGAAGGGGAGCTGCAGGCCTGTGCTGATGTCGTGGATCGAGAACGCTTCTGCCGCTGGGCGGGCCTA
  CCTCGACAGGGCTTTCCCATCATCTTTCACGGCGTAATGGGCAAAGATGAGCGTGAAGGCAACAGCCCA
  TCCTTCTTCAACCCTGAAGAGGCTGCCACAGTGACTTCCTACCTGAAGCTGCTCCTGGCCCCCTCCTCC
  AAGAAGGGCAAAGCTCGCCTGAGCCCTCGAAGTGTGGGCGTCATCTCCCCGTACCGGAAACAGGTGGAG
  AAAATCCGTTACTGCATCACCAAACTTGACAGGGAGCTTCGAGGACTGGATGACATCAAGGACTTGAAG
  GTGGGTTCAGTAGAAGAATTCCAAGGCCAAGAACGAAGCGTCATCCTCATCTCCACCGTGCGAAGCAGC
  CAGAGCTTTGTGCAGCTGGATCTGGACTTTAATCTGGGTTTCCTTAAGAACCCCAAGAGGTTCAATGTA
  GCTGTGACCCGGGCCAAGGCCCTGCTCATCATCGTGGGGAACCCCCTTCTCCTGGGCCATGACCCTGAC
  TGGAAAGTATTCCTGGAGTTCTGTAAAGAAAACGGAGGGTATACCGGGTGTCCCTTCCCTGCCAAACTG
  GACCTGCAACAGGGACAGAATTTACTGCAAGGTCTGAGCAAGCTCAGCCCCTCTACCTCAGGGCCCCAC
  AGCCATGACTACCTCCCCCAGGAGCGGGAGGGTGAAGGGGGCCTGTCTCTGCAAGTGGAGCCAGAGTGG
  AGGAATGAGCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239812
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLYGMKIANLAYVTKTRVRFFRLDRWADVRFPEKRRMKLGSDISKHHKSLLAKIFYDRAEYLHGKHGVD
  VEVQGPHEARDGQLLIRLDLNRKEVLTLRLRNGGTQSVTLTHLFPLCRTPQFAFYNEDQELPCPLGPGE
  CYELHVHCKTSFVGYFPATVLWELLGPGESGSEGAGTFYIARFLAAVAHSPLAAQLKPMTPFKRTRITG
  NPVVTNRIEEGERPDRAKGYDLELSMALGTYYPPPRLRQLLPMLLQGTSIFTAPKEIAEIKAQLETALK
  WRNYEVKLRLLLHLEELQMEHDIRHYDLESVPMTWDPVDQNPRLLTLEVPGVTESRPSVLRGDHLFALL
  SSETHQEDPITYKGFVHKVELDRVKLSFSMSLLSRFVDGLTFKVNFTFNRQPLRVQHRALELTGRWLLW
  PMLFPVAPRDVPLLPSDVKLKLYDRSLESNPEQLQAMRHIVTGTTRPAPYIIFGPPGTGKTVTLVEAIK
  QVVKHLPKAHILACAPSNSGADLLCQRLRVHLPSSIYRLLAPSRDIRMVPEDIKPCCNWDAKKGEYVFP
  AKKKLQEYRVLITTLITAGRLVSAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMEVKETGDPGGQLVL
  AGDPRQLGPVLRSPLTQKHGLGYSLLERLLTYNSLYKKGPDGYDPQFITKLLRNYRSHPTILDIPNQLY
  YEGELQACADVVDRERFCRWAGLPRQGFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKLLLAPSS
  KKGKARLSPRSVGVISPYRKQVEKIRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVILISTVRSS
  QSFVQLDLDFNLGFLKNPKRFNVAVTRAKALLIIVGNPLLLGHDPDWKVFLEFCKENGGYTGCPFPAKL
  DLQQGQNLLQGLSKLSPSTSGPHSHDYLPQEREGEGGLSLQVEPEWRNEL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286072
• ORF Size: 2841 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001286072.1, NP_001273001.1
• RefSeq Size: 4515 bp
• RefSeq ORF: 2844 bp
• Locus ID: 4343
• UniProt ID: Q9HCE1
• Cytogenetics: 1p13.2
• MW: 107.7 kDa
• Gene Summary: 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed:24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:16289642, PubMed:17507929, PubMed:22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:30122351, PubMed:23093941). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed:30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed:24726324).[UniProtKB/Swiss-Prot Function]