MOV10 (NM_001286072) Human Tagged ORF Clone

CAT#: RC239812

MOV10 (myc-DDK-tagged) - Human Mov10 RISC complex RNA helicase (MOV10), transcript variant 3

Product Images

Circular map for RC239812

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOV10
• Synonyms: fSAP113; gb110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239812 representing NM_001286072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTATGGAATGAAGATTGCAAATCTGGCCTACGTCACCAAGACTCGGGTCAGGTTCTTCAGACTCG
  ACCGCTGGGCCGACGTGCGGTTCCCAGAAAAGAGGAGAATGAAGCTGGGGTCAGATATCAGCAAACACCA
  CAAGTCACTGCTAGCCAAGATCTTTTATGACAGGGCTGAGTATCTTCATGGGAAACATGGTGTGGATGTG
  GAAGTCCAGGGGCCCCATGAAGCCCGAGATGGGCAGCTCCTTATCCGCCTGGATTTGAACCGCAAAGAGG
  TGCTGACCCTGAGGCTTCGGAATGGCGGAACCCAGTCTGTTACCCTCACTCACCTCTTCCCACTCTGCCG
  GACACCCCAGTTTGCTTTCTACAATGAAGACCAGGAGTTGCCCTGTCCACTGGGCCCCGGTGAATGCTAT
  GAACTCCATGTCCATTGTAAGACCAGCTTTGTGGGCTACTTCCCAGCCACAGTGCTCTGGGAGCTGCTGG
  GACCTGGGGAGTCGGGTTCAGAAGGAGCCGGCACATTCTACATTGCCCGCTTCTTGGCTGCCGTCGCCCA
  CAGCCCCCTGGCTGCACAGCTGAAGCCCATGACTCCCTTCAAGCGGACCCGGATCACCGGAAACCCTGTG
  GTGACCAATCGGATAGAGGAAGGAGAGAGACCTGACCGCGCTAAGGGCTATGACCTGGAGTTAAGTATGG
  CGCTGGGGACATACTACCCACCTCCCCGCCTCAGGCAGCTGCTCCCCATGCTTCTTCAGGGAACAAGTAT
  CTTCACTGCCCCTAAGGAGATCGCAGAGATCAAGGCCCAGCTGGAGACAGCCCTGAAGTGGAGGAACTAT
  GAGGTGAAGCTGCGGCTGCTGCTGCACCTGGAGGAACTGCAGATGGAGCATGATATCCGGCACTATGACC
  TGGAGTCGGTGCCCATGACCTGGGACCCTGTGGACCAGAACCCCAGGCTGCTCACGCTGGAGGTTCCTGG
  AGTGACTGAGAGCCGCCCCTCAGTGCTACGGGGCGACCACCTGTTTGCCCTTTTGTCCTCGGAGACACAC
  CAGGAGGACCCCATCACATATAAGGGCTTTGTGCACAAGGTGGAATTGGACCGTGTCAAGCTGAGCTTTT
  CCATGAGCCTCCTGAGCCGCTTTGTGGATGGGCTGACCTTCAAGGTGAACTTTACCTTCAACCGCCAGCC
  GCTGCGAGTCCAGCACCGTGCCCTGGAGCTGACAGGGCGCTGGCTGCTGTGGCCCATGCTCTTTCCTGTG
  GCACCTCGGGACGTCCCGCTGCTGCCCTCAGATGTGAAACTCAAGCTGTACGACCGGAGTCTGGAGTCAA
  ACCCAGAGCAGCTGCAGGCCATGAGGCACATTGTTACGGGCACCACCCGTCCAGCCCCCTACATCATCTT
  TGGGCCTCCAGGCACCGGCAAGACTGTCACGTTAGTGGAGGCAATTAAGCAGGTGGTGAAGCACTTGCCC
  AAAGCCCACATCTTGGCCTGCGCTCCATCCAACTCAGGGGCTGACCTACTCTGTCAAAGGCTCCGGGTCC
  ACCTTCCTAGCTCCATCTACCGCCTCCTGGCCCCCAGCAGGGACATCCGCATGGTACCTGAGGACATCAA
  GCCCTGCTGCAACTGGGACGCAAAGAAGGGGGAGTATGTATTTCCCGCCAAGAAGAAGCTGCAGGAATAC
  CGGGTCTTAATTACCACCCTCATCACTGCCGGCAGGTTGGTCTCGGCCCAGTTTCCCATTGATCACTTCA
  CACACATCTTCATCGATGAGGCTGGCCACTGCATGGAGCCTGAGAGTCTGGTAGCTATAGCAGGGCTGAT
  GGAAGTAAAGGAAACAGGTGATCCAGGAGGGCAGCTGGTGCTGGCAGGAGACCCTCGGCAGCTGGGGCCT
  GTGCTGCGTTCCCCACTGACCCAGAAGCATGGACTGGGATACTCACTGCTGGAGCGGCTGCTCACCTACA
  ACTCCCTGTACAAGAAGGGCCCTGATGGCTATGACCCCCAGTTCATAACCAAGCTGCTCCGCAACTACAG
  GTCTCATCCCACCATCCTGGACATTCCTAACCAGCTCTATTATGAAGGGGAGCTGCAGGCCTGTGCTGAT
  GTCGTGGATCGAGAACGCTTCTGCCGCTGGGCGGGCCTACCTCGACAGGGCTTTCCCATCATCTTTCACG
  GCGTAATGGGCAAAGATGAGCGTGAAGGCAACAGCCCATCCTTCTTCAACCCTGAAGAGGCTGCCACAGT
  GACTTCCTACCTGAAGCTGCTCCTGGCCCCCTCCTCCAAGAAGGGCAAAGCTCGCCTGAGCCCTCGAAGT
  GTGGGCGTCATCTCCCCGTACCGGAAACAGGTGGAGAAAATCCGTTACTGCATCACCAAACTTGACAGGG
  AGCTTCGAGGACTGGATGACATCAAGGACTTGAAGGTGGGTTCAGTAGAAGAATTCCAAGGCCAAGAACG
  AAGCGTCATCCTCATCTCCACCGTGCGAAGCAGCCAGAGCTTTGTGCAGCTGGATCTGGACTTTAATCTG
  GGTTTCCTTAAGAACCCCAAGAGGTTCAATGTAGCTGTGACCCGGGCCAAGGCCCTGCTCATCATCGTGG
  GGAACCCCCTTCTCCTGGGCCATGACCCTGACTGGAAAGTATTCCTGGAGTTCTGTAAAGAAAACGGAGG
  GTATACCGGGTGTCCCTTCCCTGCCAAACTGGACCTGCAACAGGGACAGAATTTACTGCAAGGTCTGAGC
  AAGCTCAGCCCCTCTACCTCAGGGCCCCACAGCCATGACTACCTCCCCCAGGAGCGGGAGGGTGAAGGGG
  GCCTGTCTCTGCAAGTGGAGCCAGAGTGGAGGAATGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239812 representing NM_001286072
  Red=Cloning site Green=Tags(s)
  
  MLYGMKIANLAYVTKTRVRFFRLDRWADVRFPEKRRMKLGSDISKHHKSLLAKIFYDRAEYLHGKHGVDV
  EVQGPHEARDGQLLIRLDLNRKEVLTLRLRNGGTQSVTLTHLFPLCRTPQFAFYNEDQELPCPLGPGECY
  ELHVHCKTSFVGYFPATVLWELLGPGESGSEGAGTFYIARFLAAVAHSPLAAQLKPMTPFKRTRITGNPV
  VTNRIEEGERPDRAKGYDLELSMALGTYYPPPRLRQLLPMLLQGTSIFTAPKEIAEIKAQLETALKWRNY
  EVKLRLLLHLEELQMEHDIRHYDLESVPMTWDPVDQNPRLLTLEVPGVTESRPSVLRGDHLFALLSSETH
  QEDPITYKGFVHKVELDRVKLSFSMSLLSRFVDGLTFKVNFTFNRQPLRVQHRALELTGRWLLWPMLFPV
  APRDVPLLPSDVKLKLYDRSLESNPEQLQAMRHIVTGTTRPAPYIIFGPPGTGKTVTLVEAIKQVVKHLP
  KAHILACAPSNSGADLLCQRLRVHLPSSIYRLLAPSRDIRMVPEDIKPCCNWDAKKGEYVFPAKKKLQEY
  RVLITTLITAGRLVSAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMEVKETGDPGGQLVLAGDPRQLGP
  VLRSPLTQKHGLGYSLLERLLTYNSLYKKGPDGYDPQFITKLLRNYRSHPTILDIPNQLYYEGELQACAD
  VVDRERFCRWAGLPRQGFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKLLLAPSSKKGKARLSPRS
  VGVISPYRKQVEKIRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVILISTVRSSQSFVQLDLDFNL
  GFLKNPKRFNVAVTRAKALLIIVGNPLLLGHDPDWKVFLEFCKENGGYTGCPFPAKLDLQQGQNLLQGLS
  KLSPSTSGPHSHDYLPQEREGEGGLSLQVEPEWRNEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286072
• ORF Size: 2841 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286072.1, NP_001273001.1
• RefSeq Size: 4515 bp
• RefSeq ORF: 2844 bp
• Locus ID: 4343
• UniProt ID: Q9HCE1
• Cytogenetics: 1p13.2
• MW: 107.7 kDa
• Gene Summary: 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed:24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:16289642, PubMed:17507929, PubMed:22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:30122351, PubMed:23093941). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed:30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed:24726324).[UniProtKB/Swiss-Prot Function]