PTPLA (HACD1) (NM_014241) Human Tagged ORF Clone

CAT#: RG203054

PTPLA (tGFP-tagged) - Human protein tyrosine phosphatase-like (proline instead of catalytic arginine), member A (PTPLA)

Product Images

Circular map for RG203054

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPLA
• Synonyms: CAP; PTPLA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203054 representing NM_014241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGCCTGACGGAAGCGGCGGCAGCGGGCAGCGGCTCTCGGGCTGCAGGCTGGGCAGGGTCCCCTC
  CCACGCTCCTGCCGCTGTCTCCCACGTCCCCCAGGTGCGCGGCCACCATGGCGTCCAGCGACGAGGACGG
  CACCAACGGCGGCGCCTCGGAGGCCGGCGAGGACCGGGAGGCTCCCGGCAAGCGGAGGCGCCTGGGGTTC
  TTGGCCACCGCCTGGCTCACCTTCTACGACATCGCCATGACCGCGGGGTGGTTGGTTCTAGCTATTGCCA
  TGGTACGTTTTTATATGGAAAAAGGAACACACAGAGGTTTATATAAAAGTATTCAGAAGACACTTAAATT
  TTTCCAGACATTTGCCTTGCTTGAGATAGTTCACTGTTTAATTGGAATTGTACCTACTTCTGTGATTGTG
  ACTGGGGTCCAAGTGAGTTCAAGAATCTTTATGGTGTGGCTCATTACTCACAGTATAAAACCAATCCAGA
  ATGAAGAGAGTGTGGTGCTTTTTCTGGTCGCGTGGACTGTGACAGAGATCACTCGCTATTCCTTCTACAC
  ATTCAGCCTTCTTGACCACTTGCCATACTTCATTAAATGGGCCAGATATAATTTTTTTATCATCTTATAT
  CCTGTTGGAGTTGCTGGTGAACTTCTTACAATATACGCTGCCTTGCCGCATGTGAAGAAAACAGGAATGT
  TTTCAATAAGACTTCCTAACAAATACAATGTCTCTTTTGACTACTATTATTTTCTTCTTATAACCATGGC
  ATCATATATACCTTTGTTTCCACAACTCTATTTTCATATGTTACGTCAAAGAAGAAAGGTGCTTCATGGA
  GAGGTGATTGTAGAAAAGGATGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203054 representing NM_014241
  Red=Cloning site Green=Tags(s)
  
  MGRLTEAAAAGSGSRAAGWAGSPPTLLPLSPTSPRCAATMASSDEDGTNGGASEAGEDREAPGKRRRLGF
  LATAWLTFYDIAMTAGWLVLAIAMVRFYMEKGTHRGLYKSIQKTLKFFQTFALLEIVHCLIGIVPTSVIV
  TGVQVSSRIFMVWLITHSIKPIQNEESVVLFLVAWTVTEITRYSFYTFSLLDHLPYFIKWARYNFFIILY
  PVGVAGELLTIYAALPHVKKTGMFSIRLPNKYNVSFDYYYFLLITMASYIPLFPQLYFHMLRQRRKVLHG
  EVIVEKDD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014241
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014241.2, NP_055056.2
• RefSeq Size: 1026 bp
• RefSeq ORF: 867 bp
• Locus ID: 9200
• UniProt ID: B0YJ81
• Cytogenetics: 10p12.33
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• Gene Summary: The protein encoded by this gene contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation. [provided by RefSeq, Jul 2008]