PER3 (NM_001289864) Human Tagged ORF Clone

CAT#: RC239744

PER3 (myc-DDK-tagged) - Human period circadian clock 3 (PER3), transcript variant 5

Product Images

Circular map for RC239744

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PER3
• Synonyms: FASPS3; GIG13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239744 representing NM_001289864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGCCATACACCAAAAAGTTTTGAAGTATGCAGGGCATCCTCCCTTTGAACATTCTCCCATTCGAT
  TTTGTACTCAAAACGGAGACTACATCATACTGGATTCCAGTTGGTCCAGCTTTGTGAATCCCTGGAGCCG
  GAAGATTTCTTTCATCATTGGTCGGCATAAAGTTCGAACGAGCCCACTAAATGAGGATGTTTTTGCTACC
  AAAATTAAAAAGATGAACGATAATGACAAAGACATAACAGAATTACAAGAACAAATTTACAAACTTCTCT
  TACAGCCAGTTCACGTGAGCGTGTCCAGCGGCTACGGGAGCCTGGGGAGCAGCGGGTCGCAGGAGCAGCT
  TGTCAGCATCGCCTCCTCCAGTGAGGCCAGTGGGCACCGTGTGGAGGAGACGAAGGCGGAGCAGATGACC
  TTGCAGCAGGTCTATGCCAGTGTGAACAAAATTAAAAATCTGGGTCAGCAGCTCTACATTGAGTCAATGA
  CCAAATCATCATTCAAGCCAGTGACGGGGACACGCACAGAACCGAATGGTGGTGGTGAGTCAGCGAATGG
  TGGTGGTGAATGTAAGACCTTTACTTCCTTCCACCAAACACTGAAAAACAATAGTGTGTACACTGAGCCC
  TGTGAGGATTTGAGGAACGATGAGCACAGCCCATCCTATCAACAGATCAACTGTATCGACAGTGTCATCA
  GATACCTGAAGAGCTACAACATTCCAGCTTTGAAAAGAAAGTGTATCTCCTGTACAAATACAACTTCTTC
  CTCCTCAGAAGAAGACAAACAGAACCACAAGGCAGATGATGTCCAAGCCTTACAAGCTGGTTTGCAAATC
  CCAGCCATACCTAAATCAGAAATGCCAACAAATGGACGGTCCATAGACACAGGAGGAGGAGCTCCACAGA
  TCCTGTCCACGGCGATGCTGAGCTTGGGGTCGGGCATAAGCCAATGCGGTTACAGCAGCACCATTGTCCA
  TGTCCCACCCCCAGAGACAGCCAGGGATGCTACCCTCTTCTGTGAGCCCTGGACCCTGAACATGCAGCCA
  GCCCCTTTGACCTCGGAAGAATTTAAACACGTGGGGCTCACAGCGGCTGTTCTGTCAGCGCACACCCAGA
  AGGAAGAGCAGAATTATGTTGATAAATTCCGAGAAAAGATCCTGTCATCACCCTACAGCTCCTATCTTCA
  GCAAGAAAGCAGGAGCAAAGCTAAATATTCATATTTTCAAGGAGATTCTACTTCCAAGCAGACGCGGTCG
  GCCGGCTGCAGGAAAGGGAAGCACAAGCGGAAGAAGCTGCCGGAGCCGCCAGACAGCAGCAGCTCGAACA
  CCGGCTCTGGTCCCCGCAGGGGAGCGCATCAGAACGCACAGCCCTGCTGCCCCTCCGCGGCCTCCTCTCC
  GCACACCTCGAGCCCGACCTTCCCACCTGCCGCCATGGTGCCCAGCCAGGCCCCTTACCTCGTCCCAGCT
  TTTCCCCTCCCAGCCGCGACCTCACCCGGAAGAGAATACGCAGCCCCCGGAACTGCACCGGAAGGCCTGC
  ATGGGCTGCCCTTGTCCGAGGGCTTGCAGCCTTACCCAGCTTTCCCTTTTCCTTACTTGGATACTTTTAT
  GACCGTTTTCCTGCCTGACCCCCCTGTCTGTCCTCTGTTGTCGCCATCGTTTTTGCCATGTCCATTCCTG
  GGGGCGACAGCCTCTTCTGCGATATCACCCTCAATGTCGTCAGCAATGAGTCCAACTCTGGACCCACCCC
  CTTCAGTCACCAGCCAAAGGAGAGAGGAGGAAAAGTGGGAGGCACAAAGCGAGGGGCACCCGTTCATTAC
  TTCGAGAAGCAGCTCACCCTTGCAGTTAAACTTACTTCAGGAAGAGATGCCCAGACCCTCTGAATCTCCA
  GATCAGATGAGAAGGAACACGTGCCCACAAACTGAGTATTGTGTTACAGGCAACAATGGCAGTGAGAGCA
  GTCCTGCTACTACCGGTGCACTGTCCACGGGGTCACCTCCCAGGGAGAATCCATCCCATCCTACTGCCAG
  CGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTACTGCCAGCGCTCTGTCCACAGGA
  TCGCCTCCCATGAAGAATCCATCCCATCCTACTGCCAGCACACTGTCCATGGGATTGCCTCCCAGCAGGA
  CTCCATCCCATCCTACTGCCACTGTTCTGTCCACGGGGTCACCTCCCAGCGAATCCCCATCCAGAACTGG
  TTCAGCAGCATCAGGAAGCAGCGACAGCAGTATATACCTTACTAGTAGTGTTTATTCTTCTAAAATCTCC
  CAAAATGGGCAGCAATCTCAGGACGTACAGAAAAAAGAAACATTTCCTAATGTCGCCGAAGAGCCCATCT
  GGAGAATGATACGGCAGACACCTGAGCGCATTCTCATGACATACCAGGTACCTGAGAGGGTTAAAGAAGT
  TGTACTAAAAGAAGACCTGGAAAAGCTAGAAAGTATGAGGCAGCAGCAGCCCCAGTTTTCTCATGGGCAA
  AAGGAGGAGCTGGCTAAGGTGTATAATTGGATTCAAAGCCAGACTGTCACTCAAGAAATCGACATTCAAG
  CCTGTGTCACTTGTGAAAATGAAGATTCAGCTGATGGTGCGGCCACATCCTGTGGTCAGGTTCTGGTAGA
  AGACAGCTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239744 representing NM_001289864
  Red=Cloning site Green=Tags(s)
  
  MVAIHQKVLKYAGHPPFEHSPIRFCTQNGDYIILDSSWSSFVNPWSRKISFIIGRHKVRTSPLNEDVFAT
  KIKKMNDNDKDITELQEQIYKLLLQPVHVSVSSGYGSLGSSGSQEQLVSIASSSEASGHRVEETKAEQMT
  LQQVYASVNKIKNLGQQLYIESMTKSSFKPVTGTRTEPNGGGESANGGGECKTFTSFHQTLKNNSVYTEP
  CEDLRNDEHSPSYQQINCIDSVIRYLKSYNIPALKRKCISCTNTTSSSSEEDKQNHKADDVQALQAGLQI
  PAIPKSEMPTNGRSIDTGGGAPQILSTAMLSLGSGISQCGYSSTIVHVPPPETARDATLFCEPWTLNMQP
  APLTSEEFKHVGLTAAVLSAHTQKEEQNYVDKFREKILSSPYSSYLQQESRSKAKYSYFQGDSTSKQTRS
  AGCRKGKHKRKKLPEPPDSSSSNTGSGPRRGAHQNAQPCCPSAASSPHTSSPTFPPAAMVPSQAPYLVPA
  FPLPAATSPGREYAAPGTAPEGLHGLPLSEGLQPYPAFPFPYLDTFMTVFLPDPPVCPLLSPSFLPCPFL
  GATASSAISPSMSSAMSPTLDPPPSVTSQRREEEKWEAQSEGHPFITSRSSSPLQLNLLQEEMPRPSESP
  DQMRRNTCPQTEYCVTGNNGSESSPATTGALSTGSPPRENPSHPTASALSTGSPPMKNPSHPTASALSTG
  SPPMKNPSHPTASTLSMGLPPSRTPSHPTATVLSTGSPPSESPSRTGSAASGSSDSSIYLTSSVYSSKIS
  QNGQQSQDVQKKETFPNVAEEPIWRMIRQTPERILMTYQVPERVKEVVLKEDLEKLESMRQQQPQFSHGQ
  KEELAKVYNWIQSQTVTQEIDIQACVTCENEDSADGAATSCGQVLVEDSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289864
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289864.3
• RefSeq Size: 6394 bp
• RefSeq ORF: 2673 bp
• Locus ID: 8863
• UniProt ID: P56645
• Cytogenetics: 1p36.23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 96.8 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]