PARP8 (NM_001178055) Human Tagged ORF Clone

CAT#: RC230545

PARP8 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 8 (PARP8), transcript variant 1

Product Images

Circular map for RC230545

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PARP8
• Synonyms: ARTD16; pART16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230545 representing NM_001178055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATGTGTTCAAGGCAAGAGCGAATTCAGAAGGATATCGACGTCGTGATCCAGAAGTCCAGAGCTG
  AGAAGGACTGCCTGTTTGCAGATTTCAGATACTCTGACTCCACCTTTACTTTTACCTACGTTGGCGGCCC
  CAGAAGTGTATCCTACTCAGTACATGTATCTGAAGATTACCCAGATAATACATATGTGTCAAGTTCAGAG
  AATGATGAAGATGTGCTAGTTACTACAGAGCCAATACCAGTAATTTTTCATAGAATAGCAACAGAATTAA
  GAAAAACAAATGACATTAACTGTTGCTTATCCATAAAATCCAAATTACAAAAGGAAAATGGGGAGGAATC
  AAGACAGAATAGTACAGTGGAGGAAGATTCTGAAGGTGACAATGATTCCGAAGAATTTTATTACGGAGGG
  CAGGTGAACTATGATGGGGAACTGCACAAGCACCCACAACTGGAAGCTGATTTGTCAGCAGTTAGAGAGA
  TATATGGGCCACATGCAGTTTCTCTCAGGGAATATGGAGCCATTGATGATGTAGATATTGATCTGCATAT
  CGATGTTAGCTTTCTTGATGAGGAGATTGCTGTGGCTTGGGAAGTAATTCGAACAGAACCTATAATTGTT
  CGACTACACTGTTCACTTACACAGTATTTAAATGGCCCAGTGCCCACTGTTGATGTCTTTCAGATTTCCA
  CAAAAGAGCGATTTGGATTGGGACATCAGCTGAAAAAAATCATGCAGACATTTGTTACACAGCAGTGGAA
  ACAGAGCAAAGAAAAATCCAATTGCCTGCACAATAAAAAGTTGTCAGAGAAGAAAGTGAAGTCTCCCCTG
  CATTTATTTTCTACTTTGCGCAGGTCGCCAAGTTATCCTCCCCCTGGTTGTGGCAAAAGCAAATCCAAAC
  TGAAATCTGAGCAGGACGGAATCTCCAAAACGCATAAGCTGCTGCGGAGGACTTGTTCCAGCACAGTCAA
  GACTGATGATGTGTGTGTCACAAAGTCACACAGGACCTTTGGCCGCTCCTTGTCCAGCGATCCCAGGGCG
  GAGCAGGCTATGACAGCAATTAAATCGCACAAACTTTTGAACCGTCCTTGCCCTGCAGCTGTTAAGTCAG
  AGGAATGCCTAACTCTAAAGTCGCATAGACTATTGACTCGATCTTGTTCTGGAGATCCACGATGTGAGCA
  CAACACAAACTTGAAGCCCCATAAACTGTTAAGCAGGTCTTACTCTAGTAATCTCAGAATGGAAGAATTA
  TATGGACTGAAAAATCACAAATTGCTCAGCAAGTCCTACTCCAGTGCCCCCAAGTCATCCAAAACTGAGC
  TTTTCAAGGAACCTAACGCAGAGGGCAGGAGGCTCTCTCTTACCTCAGGGCTTATTGGTATCCTAACACC
  ATCTTCATCTTCATCTTCTCAGCTTGCTCCAAATGGTGCAAAATGCATTCCAGTACGAGACCGTGGCTTC
  CTGGTGCAGACAATTGAGTTTGCTGAACAGCGGATCCCTGTATTAAATGAATATTGTGTGGTTTGTGATG
  AGCCACATGTGTTTCAAAATGGCCCTATGCTTAGGCCTACCGTATGTGAACGGGAGCTGTGTGTGTTTGC
  TTTTCAAACCCTGGGAGTAATGAATGAAGCTGCTGATGAAATAGCAACTGGAGCTCAGGTGGTAGATCTA
  CTAGTATCCATGTGTAGGTCTGCGTTGGAATCTCCTAGAAAAGTTGTGATTTTCGAGCCATATCCTTCTG
  TGGTAGATCCTAATGATCCTCAGATGTTGGCCTTCAACCCCAGGAAAAAGAACTATGATCGAGTAATGAA
  AGCACTGGATAGCATAACTTCTATCAGAGAAATGACACAAGCACCATATCTGGAAATCAAGAAGCAAATG
  GATAAACAGGACCCCCTTGCTCATCCCTTACTGCAATGGGTTATATCAAGTAATAGATCACATATTGTGA
  AACTGCCAGTTAACAGGCAATTGAAGTTTATGCATACTCCACATCAGTTCCTTCTTCTCAGCAGTCCACC
  AGCCAAAGAATCCAATTTTAGAGCTGCTAAAAAACTCTTTGGAAGCACCTTTGCATTTCATGGCTCACAC
  ATTGAAAACTGGCACTCCATCCTGAGGAATGGTCTGGTTGTTGCTTCTAATACACGATTGCAGCTCCATG
  GTGCAATGTATGGAAGTGGAATCTATCTTAGTCCAATGTCAAGCATATCATTTGGTTACTCAGGGATGAA
  CAAGAAACAGAAGGTGTCAGCCAAGGACGAGCCAGCTTCAAGCAGTAAAAGCAGCAATACATCACAGTCA
  CAGAAAAAAGGACAGCAATCCCAATTCCTGCAAAGCCGTAACTTAAAATGCATAGCCTTATGTGAAGTGA
  TCACCTCATCTGACCTGCACAAACATGGAGAGATATGGGTTGTCCCCAATACTGACCATGTCTGCACACG
  ATTCTTTTTCGTCTATGAAGACGGCCAAGTGGGAGATGCAAATATTAATACACAAGAAGGAGGCATTCAC
  AAAGAGATCCTCCGAGTAATTGGTAATCAAACTGCTACTGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230545 representing NM_001178055
  Red=Cloning site Green=Tags(s)
  
  MGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPRSVSYSVHVSEDYPDNTYVSSSE
  NDEDVLVTTEPIPVIFHRIATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGG
  QVNYDGELHKHPQLEADLSAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIV
  RLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPL
  HLFSTLRRSPSYPPPGCGKSKSKLKSEQDGISKTHKLLRRTCSSTVKTDDVCVTKSHRTFGRSLSSDPRA
  EQAMTAIKSHKLLNRPCPAAVKSEECLTLKSHRLLTRSCSGDPRCEHNTNLKPHKLLSRSYSSNLRMEEL
  YGLKNHKLLSKSYSSAPKSSKTELFKEPNAEGRRLSLTSGLIGILTPSSSSSSQLAPNGAKCIPVRDRGF
  LVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDL
  LVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQM
  DKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSH
  IENWHSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKSSNTSQS
  QKKGQQSQFLQSRNLKCIALCEVITSSDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIH
  KEILRVIGNQTATG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178055
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178055.2
• RefSeq ORF: 2565 bp
• Locus ID: 79668
• UniProt ID: Q8N3A8
• Cytogenetics: 5q11.1
• MW: 96.3 kDa
• Gene Summary: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.[UniProtKB/Swiss-Prot Function]