PARP8 (NM_001178055) Human Untagged Clone

CAT#: SC329183

PARP8 (untagged)-Human poly (ADP-ribose) polymerase family member 8 (PARP8) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARP8
• Synonyms: ARTD16; pART16
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001178055, the custom clone sequence may differ by one or more nucleotides
  ATGGGGATGTGTTCAAGGCAAGAGCGAATTCAGAAGGATATCGACGTCGTGATCCAGAAG
  TCCAGAGCTGAGAAGGACTGCCTGTTTGCAGATTTCAGATACTCTGACTCCACCTTTACT
  TTTACCTACGTTGGCGGCCCCAGAAGTGTATCCTACTCAGTACATGTATCTGAAGATTAC
  CCAGATAATACATATGTGTCAAGTTCAGAGAATGATGAAGATGTGCTAGTTACTACAGAG
  CCAATACCAGTAATTTTTCATAGAATAGCAACAGAATTAAGAAAAACAAATGACATTAAC
  TGTTGCTTATCCATAAAATCCAAATTACAAAAGGAAAATGGGGAGGAATCAAGACAGAAT
  AGTACAGTGGAGGAAGATTCTGAAGGTGACAATGATTCCGAAGAATTTTATTACGGAGGG
  CAGGTGAACTATGATGGGGAACTGCACAAGCACCCACAACTGGAAGCTGATTTGTCAGCA
  GTTAGAGAGATATATGGGCCACATGCAGTTTCTCTCAGGGAATATGGAGCCATTGATGAT
  GTAGATATTGATCTGCATATCGATGTTAGCTTTCTTGATGAGGAGATTGCTGTGGCTTGG
  GAAGTAATTCGAACAGAACCTATAATTGTTCGACTACACTGTTCACTTACACAGTATTTA
  AATGGCCCAGTGCCCACTGTTGATGTCTTTCAGATTTCCACAAAAGAGCGATTTGGATTG
  GGACATCAGCTGAAAAAAATCATGCAGACATTTGTTACACAGCAGTGGAAACAGAGCAAA
  GAAAAATCCAATTGCCTGCACAATAAAAAGTTGTCAGAGAAGAAAGTGAAGTCTCCCCTG
  CATTTATTTTCTACTTTGCGCAGGTCGCCAAGTTATCCTCCCCCTGGTTGTGGCAAAAGC
  AAATCCAAACTGAAATCTGAGCAGGACGGAATCTCCAAAACGCATAAGCTGCTGCGGAGG
  ACTTGTTCCAGCACAGTCAAGACTGATGATGTGTGTGTCACAAAGTCACACAGGACCTTT
  GGCCGCTCCTTGTCCAGCGATCCCAGGGCGGAGCAGGCTATGACAGCAATTAAATCGCAC
  AAACTTTTGAACCGTCCTTGCCCTGCAGCTGTTAAGTCAGAGGAATGCCTAACTCTAAAG
  TCGCATAGACTATTGACTCGATCTTGTTCTGGAGATCCACGATGTGAGCACAACACAAAC
  TTGAAGCCCCATAAACTGTTAAGCAGGTCTTACTCTAGTAATCTCAGAATGGAAGAATTA
  TATGGACTGAAAAATCACAAATTGCTCAGCAAGTCCTACTCCAGTGCCCCCAAGTCATCC
  AAAACTGAGCTTTTCAAGGAACCTAACGCAGAGGGCAGGAGGCTCTCTCTTACCTCAGGG
  CTTATTGGTATCCTAACACCATCTTCATCTTCATCTTCTCAGCTTGCTCCAAATGGTGCA
  AAATGCATTCCAGTACGAGACCGTGGCTTCCTGGTGCAGACAATTGAGTTTGCTGAACAG
  CGGATCCCTGTATTAAATGAATATTGTGTGGTTTGTGATGAGCCACATGTGTTTCAAAAT
  GGCCCTATGCTTAGGCCTACCGTATGTGAACGGGAGCTGTGTGTGTTTGCTTTTCAAACC
  CTGGGAGTAATGAATGAAGCTGCTGATGAAATAGCAACTGGAGCTCAGGTGGTAGATCTA
  CTAGTATCCATGTGTAGGTCTGCGTTGGAATCTCCTAGAAAAGTTGTGATTTTCGAGCCA
  TATCCTTCTGTGGTAGATCCTAATGATCCTCAGATGTTGGCCTTCAACCCCAGGAAAAAG
  AACTATGATCGAGTAATGAAAGCACTGGATAGCATAACTTCTATCAGAGAAATGACACAA
  GCACCATATCTGGAAATCAAGAAGCAAATGGATAAACAGGACCCCCTTGCTCATCCCTTA
  CTGCAATGGGTTATATCAAGTAATAGATCACATATTGTGAAACTGCCAGTTAACAGGCAA
  TTGAAGTTTATGCATACTCCACATCAGTTCCTTCTTCTCAGCAGTCCACCAGCCAAAGAA
  TCCAATTTTAGAGCTGCTAAAAAACTCTTTGGAAGCACCTTTGCATTTCATGGCTCACAC
  ATTGAAAACTGGCACTCCATCCTGAGGAATGGTCTGGTTGTTGCTTCTAATACACGATTG
  CAGCTCCATGGTGCAATGTATGGAAGTGGAATCTATCTTAGTCCAATGTCAAGCATATCA
  TTTGGTTACTCAGGGATGAACAAGAAACAGAAGGTGTCAGCCAAGGACGAGCCAGCTTCA
  AGCAGTAAAAGCAGCAATACATCACAGTCACAGAAAAAAGGACAGCAATCCCAATTCCTG
  CAAAGCCGTAACTTAAAATGCATAGCCTTATGTGAAGTGATCACCTCATCTGACCTGCAC
  AAACATGGAGAGATATGGGTTGTCCCCAATACTGACCATGTCTGCACACGATTCTTTTTC
  GTCTATGAAGACGGCCAAGTGGGAGATGCAAATATTAATACACAAGAAGGAGGCATTCAC
  AAAGAGATCCTCCGAGTAATTGGTAATCAAACTGCTACTGGTTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001178055
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178055.1, NP_001171526.1
• RefSeq Size: 7201 bp
• RefSeq ORF: 2565 bp
• Locus ID: 79668
• UniProt ID: Q8N3A8
• Cytogenetics: 5q11.1
• Gene Summary: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.