SPHKAP (NM_001142644) Human Tagged ORF Clone

CAT#: RC227959

  • TrueORF®

SPHKAP (Myc-DDK-tagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro


  "NM_001142644" in other vectors (4)

Reconstitution Protocol

USD 2,285.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Other products for "SPHKAP"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SPHKAP
Synonyms SKIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC227959 representing NM_001142644
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAAC
CGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAGGT
TCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAAATT
GGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGATGTGAACAAGGATG
AATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATCAGTTC
CATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAATCTCCAG
GCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGAGGGAACA
GACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTGGTGCAGGA
GCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTATCTTCAATC
GAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCTAGGAACGATT
ATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACCCAGGTGGAATG
GAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATATCCCACACCATTG
ATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTAGATCCCTCAGCCA
AGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTATTATCATTCAGAAGC
TTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTATTTCTCCATGATGGAT
AAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCAGGAGACCATGAAGACA
CAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTATGCTACAAATTTAGCAGA
ATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTACCCCAGGAATCTGCAGTC
AGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGATACAGTGGTTTCTCGGTCAT
GGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGATGCTGCCCCACAGCCAGGCAT
CTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCTCTCTGGAGAGAACTCCAGCAGA
CAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTGATTGGAACTATTTCCAGTCCAC
AGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCCCCAGGGAGCAGTGGTGCACTGCA
AACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTACTCCTTTCCATCTGCTTTGTGTGGC
ATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAAAGAGAAGAGGTGACATGCTCAGTGG
CTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCCATGCCCCCACTTTGTGGTTTAGCAAG
CATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAGGAGGCTGCTCTGGTTTTAACAAGGCCT
AATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAACAGGAGAATCATGGAAACTGCTTCAAAGT
CTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTGGCACATACCCTGTCCAATGTTATCCTGAG
GCATTCCATTGATGAAGTTCACCACAAAAATATGATAATCGACCCCAATGACAACAGGCATTCATCTGAA
ATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTTTTAGATGTGATATGCTTCACGTTCAAGAAGA
TGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTCCTTTCTAAGGAGACCATCAGAAGGAGGGAGAC
AGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGTCAAGCTTGGACAAAAGCCACTGAATCCTCCAGC
AGCTCTCCACTTAGCAATTCACACAACACGAGTCTTGTCATCAACAATCTTGTGGATGGCATGTATTCAA
AACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTCAAGAACCCCACGCTGCAGTCACAATTATCACGTAG
TCACAGAGTGCCCGATTCTTCAACTGCTACAACATCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGA
GAGGATACAAAAAGCCCTCATCACAGTGAGAATGAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAA
CGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAGGCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTA
CAACTGTGCCACATCTCGCATCAACGAAGTTCAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTT
CCTGCTCAATCCACGCTTCAAACAAAGCATCCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAG
CAGACACGGTCGTCTCCATGGCAACTGAAATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACC
CTGGTTTTGTGCATGGAAAAGAGGGAGTGAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAG
AGGAAGAAAGAGAGCCAGGGGAGCGGGACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCA
AGAGGAAGACGGACGAGCACCCTGAGCTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAA
CCTTGAAGATGTCCCAGATTCTGTCAATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACG
GAGTTCTCTATGGTGGACGGCATGTGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACA
GGTGGAGCCGGCTGAAGGCCTCCAGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGT
CAACAGCCTGGGCTTAATGAGCACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCG
AGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGAT
TTGAGTTACTGCTGGATTACTATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGC
GTGCCGGAAAAGTGACCACCTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATC
ACTGAGGAGTTCTACAGGTACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGAC
GGAGCAGCCAGGATTGGACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTC
GTCCATGCCAGACAGCAGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTA
GATGGCTTTGCTCAGAACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCG
GTCTCTGCAAATCTGACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCA
TGAAACGTGGGCTAGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAA
GCTGACACTGAGCCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGA
GCAGGATGTGCAGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTAC
CGAATGTAAACAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGAT
TCTAAAAAAGAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGA
GGGAAGTGCCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCT
TTCCAAAAGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGA
GGTGGAGACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGG
CTGAAGCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCAC
AGGCAGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGT
GAGCGATCCATGAGCAATGGCAACAGTAGTGCCACTAGCAGTCTTGGCATTATGGATCTGGACATTTATC
AGGAAAGCATGCCATCTTCTCCCATGATTAATGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTC
AGAAAGCACAGAGGCACCTGCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTG
ATCAACTTTGACCTGGAGCCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCCT
CTGAACTGGGGATTCCCACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGATGT
CGTGCAGCTGGTTCATCGGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGCAAA
ATGCATGAGGAGCAGAAGGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC227959 representing NM_001142644
Red=Cloning site Green=Tags(s)

MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQI
GFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQ
ADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSI
EEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPL
IKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMD
KDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAV
SVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSR
QPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCG
MTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRP
NTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSE
ILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSS
SSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAG
EDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLL
PAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLK
RKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLT
EFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQS
SCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESI
TEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSL
DGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEE
ADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLD
SKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVR
GGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLS
ERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLV
INFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCK
MHEEQKDGRLSLFDWLLELG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001142644
ORF Size 5100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001142644.2
RefSeq ORF 5103 bp
Locus ID 80309
UniProt ID Q2M3C7
Cytogenetics 2q36.3
Protein Families Druggable Genome
MW 186.3 kDa
Gene Summary Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.[UniProtKB/Swiss-Prot Function]

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