SPHKAP (NM_001142644) Human Untagged Clone

CAT#: SC325420

SPHKAP (untagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPHKAP
• Synonyms: SKIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325420 representing NM_001142644.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAA
  CCGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAG
  GTTCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAA
  ATTGGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGATGTGAACAAG
  GATGAATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATC
  AGTTCCATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAAT
  CTCCAGGCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGA
  GGGAACAGACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTG
  GTGCAGGAGCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTA
  TCTTCAATCGAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCT
  AGGAACGATTATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACC
  CAGGTGGAATGGAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATAT
  CCCACACCATTGATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTA
  GATCCCTCAGCCAAGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTAT
  TATCATTCAGAAGCTTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTAT
  TTCTCCATGATGGATAAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCA
  GGAGACCATGAAGACACAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTAT
  GCTACAAATTTAGCAGAATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTA
  CCCCAGGAATCTGCAGTCAGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGAT
  ACAGTGGTTTCTCGGTCATGGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGAT
  GCTGCCCCACAGCCAGGCATCTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCTC
  TCTGGAGAGAACTCCAGCAGACAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTG
  ATTGGAACTATTTCCAGTCCACAGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCC
  CCAGGGAGCAGTGGTGCACTGCAAACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTAC
  TCCTTTCCATCTGCTTTGTGTGGCATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAA
  AGAGAAGAGGTGACATGCTCAGTGGCTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCC
  ATGCCCCCACTTTGTGGTTTAGCAAGCATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAG
  GAGGCTGCTCTGGTTTTAACAAGGCCTAATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAAC
  AGGAGAATCATGGAAACTGCTTCAAAGTCTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTG
  GCACATACCCTGTCCAATGTTATCCTGAGGCATTCCATTGATGAAGTTCACCACAAAAATATGATAATC
  GACCCCAATGACAACAGGCATTCATCTGAAATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTT
  TTAGATGTGATATGCTTCACGTTCAAGAAGATGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTC
  CTTTCTAAGGAGACCATCAGAAGGAGGGAGACAGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGT
  CAAGCTTGGACAAAAGCCACTGAATCCTCCAGCAGCTCTCCACTTAGCAATTCACACAACACGAGTCTT
  GTCATCAACAATCTTGTGGATGGCATGTATTCAAAACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTC
  AAGAACCCCACGCTGCAGTCACAATTATCACGTAGTCACAGAGTGCCCGATTCTTCAACTGCTACAACA
  TCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGAGAGGATACAAAAAGCCCTCATCACAGTGAGAAT
  GAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAACGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAG
  GCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTACAACTGTGCCACATCTCGCATCAACGAAGTT
  CAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTTCCTGCTCAATCCACGCTTCAAACAAAGCAT
  CCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAGCAGACACGGTCGTCTCCATGGCAACTGAA
  ATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACCCTGGTTTTGTGCATGGAAAAGAGGGAGT
  GAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAGAGGAAGAAAGAGAGCCAGGGGAGCGGG
  ACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCAAGAGGAAGACGGACGAGCACCCTGAG
  CTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAACCTTGAAGATGTCCCAGATTCTGTC
  AATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACGGAGTTCTCTATGGTGGACGGCATG
  TGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACAGGTGGAGCCGGCTGAAGGCCTCC
  AGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGTCAACAGCCTGGGCTTAATGAGC
  ACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCGAGCTGTGAGAGCATCACCGAT
  GAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGATTTGAGTTACTGCTGGATTAC
  TATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGCGTGCCGGAAAAGTGACCAC
  CTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATCACTGAGGAGTTCTACAGG
  TACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGACGGAGCAGCCAGGATTGG
  ACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTCGTCCATGCCAGACAGC
  AGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTAGATGGCTTTGCTCAG
  AACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCGGTCTCTGCAAATCT
  GACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCATGAAACGTGGGCT
  AGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAAGCTGACACTGAG
  CCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGAGCAGGATGTGC
  AGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTACCGAATGTAAA
  CAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGATTCTAAAAAA
  GAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGAGGGAAGTG
  CCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCTTTCCAAA
  AGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGAGGTGGA
  GACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGGCTGAA
  GCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCACAGGC
  AGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGTGAG
  CGATCCATGAGCAATGGCAACAGTAGTGCCACTAGCAGTCTTGGCATTATGGATCTGGACATTTATCAG
  GAAAGCATGCCATCTTCTCCCATGATTAATGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTCA
  GAAAGCACAGAGGCACCTGCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTG
  ATCAACTTTGACCTGGAGCCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCC
  TCTGAACTGGGGATTCCCACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGAT
  GTCGTGCAGCTGGTTCATCGGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGC
  AAAATGCATGAGGAGCAGAAGGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142644
• Insert Size: 5103 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142644.1
• RefSeq Size: 6913 bp
• RefSeq ORF: 5103 bp
• Locus ID: 80309
• UniProt ID: Q2M3C7
• Cytogenetics: 2q36.3
• Protein Families: Druggable Genome
• MW: 186.5 kDa
• Gene Summary: Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).