Neurofilament (NEFM) (NM_001105541) Human Tagged ORF Clone

CAT#: RC225929

NEFM (Myc-DDK-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 2. Note: ORF is codon optimized

Product Images

Circular map for RC225929

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neurofilament
• Synonyms: NEF3; NF-M; NFM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225929 ORF sequence, codon optimized.
  Due to the complexity of NM_001105541, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGACATCTTAGAGAGTACCAGGATCTCCTTAACGTCAAGATGGCGCTGGATATAGAAATTGCCG
  CCTATCGCAAGCTTCTGGAGGGCGAGGAAACAAGATTTTCCACCTTTGCCGGGTCAATCACCGGCCCCCT
  GTACACTCACAGACCACCCATTACCATCTCTTCCAAAATTCAGAAGCCAAAGGTCGAGGCTCCAAAATTG
  AAGGTGCAACACAAGTTTGTTGAAGAGATCATTGAGGAAACAAAAGTGGAAGACGAGAAGAGCGAGATGG
  AGGAGGCCCTCACGGCCATCACCGAAGAGCTCGCTGTCAGCATGAAGGAAGAGAAAAAGGAAGCCGCCGA
  AGAGAAAGAGGAGGAACCAGAAGCTGAGGAAGAGGAGGTGGCTGCCAAGAAGAGTCCAGTCAAGGCCACT
  GCCCCTGAGGTGAAAGAGGAGGAGGGGGAGAAAGAGGAAGAGGAAGGCCAGGAAGAGGAGGAGGAAGAAG
  ATGAAGGAGCCAAGAGCGACCAGGCCGAAGAAGGAGGGAGCGAAAAAGAAGGCAGCTCCGAAAAAGAGGA
  GGGGGAGCAGGAGGAAGGCGAAACTGAGGCGGAGGCCGAAGGCGAAGAGGCCGAAGCTAAAGAGGAGAAA
  AAGGTCGAAGAGAAGAGCGAAGAGGTCGCCACGAAGGAGGAGCTGGTAGCCGATGCTAAGGTTGAGAAAC
  CTGAAAAAGCTAAATCTCCCGTGCCCAAGTCACCGGTGGAGGAGAAGGGAAAATCTCCCGTACCCAAAAG
  CCCGGTTGAAGAAAAAGGCAAAAGTCCCGTCCCTAAGAGTCCCGTGGAGGAGAAAGGTAAATCACCAGTG
  CCTAAGTCACCAGTGGAAGAGAAAGGCAAGAGTCCTGTGAGTAAGAGTCCTGTTGAGGAAAAGGCTAAGT
  CCCCGGTCCCCAAGTCCCCGGTTGAGGAGGCCAAGTCAAAGGCCGAGGTCGGAAAAGGAGAACAAAAAGA
  GGAGGAGGAGAAGGAAGTCAAAGAAGCACCTAAGGAAGAAAAAGTGGAGAAAAAGGAGGAGAAGCCTAAA
  GATGTGCCCGAGAAGAAGAAGGCTGAAAGTCCAGTGAAAGAGGAGGCAGTAGCGGAGGTTGTCACGATCA
  CAAAGAGCGTGAAGGTGCATCTGGAAAAGGAGACAAAGGAGGAAGGCAAGCCACTTCAACAGGAGAAAGA
  GAAGGAGAAGGCAGGCGGTGAGGGCGGAAGTGAAGAAGAAGGGTCAGATAAGGGCGCTAAAGGTTCTCGA
  AAGGAGGATATCGCCGTTAACGGAGAGGTAGAAGGTAAAGAGGAGGTCGAGCAGGAGACTAAGGAAAAAG
  GCTCTGGGAGGGAGGAAGAGAAAGGAGTGGTCACGAACGGACTGGATCTCAGCCCTGCCGACGAGAAGAA
  GGGCGGAGATAAAAGTGAAGAGAAGGTGGTAGTCACTAAGACTGTAGAAAAAATTACCTCTGAAGGAGGG
  GACGGCGCCACAAAATACATCACAAAGTCTGTAACCGTGACTCAAAAAGTGGAGGAACATGAAGAGACTT
  TTGAAGAGAAACTGGTGTCTACCAAGAAAGTCGAGAAAGTGACCTCCCATGCTATAGTGAAGGAGGTGAC
  TCAATCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225929 representing NM_001105541
  Red=Cloning site Green=Tags(s)
  
  MARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRPPITISSKIQKPKVEAPKL
  KVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKAT
  APEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEK
  KVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPV
  PKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPK
  DVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKGSR
  KEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPADEKKGGDKSEEKVVVTKTVEKITSEGG
  DGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105541
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105541.1, NP_001099011.1
• RefSeq Size: 2514 bp
• RefSeq ORF: 1623 bp
• Locus ID: 4741
• Cytogenetics: 8p21.2
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• MW: 59.5 kDa
• Gene Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]