Human Neurofilament (NEFM) (NM_001105541) AAV Particle

CAT#: RC225929A1V

AAV ORF Particles, serotype AAV-2, NEFM (Myc-DDK-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 2. Note: ORF is codon optimized, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Neurofilament
• Synonyms: NEF3; NF-M; NFM
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225929 ORF sequence, codon optimized.
  Due to the complexity of NM_001105541, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGACATCTTAGAGAGTACCAGGATCTCCTTAACGTCAAGATGGCGCTGGATATAGAAATTGCCG
  CCTATCGCAAGCTTCTGGAGGGCGAGGAAACAAGATTTTCCACCTTTGCCGGGTCAATCACCGGCCCCCT
  GTACACTCACAGACCACCCATTACCATCTCTTCCAAAATTCAGAAGCCAAAGGTCGAGGCTCCAAAATTG
  AAGGTGCAACACAAGTTTGTTGAAGAGATCATTGAGGAAACAAAAGTGGAAGACGAGAAGAGCGAGATGG
  AGGAGGCCCTCACGGCCATCACCGAAGAGCTCGCTGTCAGCATGAAGGAAGAGAAAAAGGAAGCCGCCGA
  AGAGAAAGAGGAGGAACCAGAAGCTGAGGAAGAGGAGGTGGCTGCCAAGAAGAGTCCAGTCAAGGCCACT
  GCCCCTGAGGTGAAAGAGGAGGAGGGGGAGAAAGAGGAAGAGGAAGGCCAGGAAGAGGAGGAGGAAGAAG
  ATGAAGGAGCCAAGAGCGACCAGGCCGAAGAAGGAGGGAGCGAAAAAGAAGGCAGCTCCGAAAAAGAGGA
  GGGGGAGCAGGAGGAAGGCGAAACTGAGGCGGAGGCCGAAGGCGAAGAGGCCGAAGCTAAAGAGGAGAAA
  AAGGTCGAAGAGAAGAGCGAAGAGGTCGCCACGAAGGAGGAGCTGGTAGCCGATGCTAAGGTTGAGAAAC
  CTGAAAAAGCTAAATCTCCCGTGCCCAAGTCACCGGTGGAGGAGAAGGGAAAATCTCCCGTACCCAAAAG
  CCCGGTTGAAGAAAAAGGCAAAAGTCCCGTCCCTAAGAGTCCCGTGGAGGAGAAAGGTAAATCACCAGTG
  CCTAAGTCACCAGTGGAAGAGAAAGGCAAGAGTCCTGTGAGTAAGAGTCCTGTTGAGGAAAAGGCTAAGT
  CCCCGGTCCCCAAGTCCCCGGTTGAGGAGGCCAAGTCAAAGGCCGAGGTCGGAAAAGGAGAACAAAAAGA
  GGAGGAGGAGAAGGAAGTCAAAGAAGCACCTAAGGAAGAAAAAGTGGAGAAAAAGGAGGAGAAGCCTAAA
  GATGTGCCCGAGAAGAAGAAGGCTGAAAGTCCAGTGAAAGAGGAGGCAGTAGCGGAGGTTGTCACGATCA
  CAAAGAGCGTGAAGGTGCATCTGGAAAAGGAGACAAAGGAGGAAGGCAAGCCACTTCAACAGGAGAAAGA
  GAAGGAGAAGGCAGGCGGTGAGGGCGGAAGTGAAGAAGAAGGGTCAGATAAGGGCGCTAAAGGTTCTCGA
  AAGGAGGATATCGCCGTTAACGGAGAGGTAGAAGGTAAAGAGGAGGTCGAGCAGGAGACTAAGGAAAAAG
  GCTCTGGGAGGGAGGAAGAGAAAGGAGTGGTCACGAACGGACTGGATCTCAGCCCTGCCGACGAGAAGAA
  GGGCGGAGATAAAAGTGAAGAGAAGGTGGTAGTCACTAAGACTGTAGAAAAAATTACCTCTGAAGGAGGG
  GACGGCGCCACAAAATACATCACAAAGTCTGTAACCGTGACTCAAAAAGTGGAGGAACATGAAGAGACTT
  TTGAAGAGAAACTGGTGTCTACCAAGAAAGTCGAGAAAGTGACCTCCCATGCTATAGTGAAGGAGGTGAC
  TCAATCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225929 representing NM_001105541
  Red=Cloning site Green=Tags(s)
  
  MARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRPPITISSKIQKPKVEAPKL
  KVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKAT
  APEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEK
  KVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPV
  PKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPK
  DVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKGSR
  KEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPADEKKGGDKSEEKVVVTKTVEKITSEGG
  DGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001105541
• ORF Size: 1620 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001105541.1, NP_001099011.1
• RefSeq Size: 2514 bp
• RefSeq ORF: 1623 bp
• Locus ID: 4741
• Cytogenetics: 8p21.2
• MW: 59.5 kDa