Neurofilament (NEFM) (NM_001105541) Human Untagged Clone

CAT#: SC317116

NEFM (untagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEFM
• Synonyms: NEF3; NF-M; NFM
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001105541, the custom clone sequence may differ by one or more nucleotides
  ATGGCTCGTCATTTGCGCGAATACCAGGACCTCCTCAACGTCAAGATGGCTCTGGATATA
  GAAATCGCTGCGTACAGAAAACTCCTGGAGGGTGAAGAGACTAGATTTAGCACATTTGCA
  GGAAGCATCACTGGGCCACTGTATACACACCGACCCCCAATCACAATATCCAGTAAGATT
  CAGAAACCCAAGGTGGAAGCTCCCAAGCTTAAGGTCCAACACAAATTTGTCGAGGAGATC
  ATAGAGGAAACCAAAGTGGAGGATGAGAAGTCAGAAATGGAAGAGGCCCTGACAGCCATT
  ACAGAGGAATTGGCCGTTTCCATGAAGGAAGAGAAGAAAGAAGCAGCAGAAGAAAAGGAA
  GAGGAACCCGAAGCTGAAGAAGAAGAAGTAGCTGCCAAAAAGTCTCCAGTGAAAGCAACT
  GCACCTGAAGTTAAAGAAGAGGAAGGGGAAAAGGAGGAAGAAGAAGGCCAGGAAGAAGAG
  GAGGAAGAAGATGAGGGAGCTAAGTCAGACCAAGCCGAAGAGGGAGGATCCGAGAAGGAA
  GGCTCTAGTGAAAAAGAGGAAGGTGAGCAGGAAGAAGGAGAAACAGAAGCTGAAGCTGAA
  GGAGAGGAAGCCGAAGCTAAAGAGGAAAAGAAAGTGGAGGAAAAGAGTGAGGAAGTGGCT
  ACCAAGGAGGAGCTGGTGGCAGATGCCAAGGTGGAAAAGCCAGAAAAAGCCAAGTCTCCT
  GTGCCAAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAA
  GAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTG
  CCGAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGTCAAAATCACCAGTGGAAGAG
  AAAGCCAAATCTCCTGTGCCAAAATCACCAGTGGAAGAGGCAAAGTCAAAAGCAGAAGTG
  GGGAAAGGTGAACAGAAAGAGGAAGAAGAAAAGGAAGTCAAGGAAGCTCCCAAGGAAGAG
  AAGGTAGAGAAAAAGGAAGAGAAACCAAAGGATGTGCCAGAGAAGAAGAAAGCTGAGTCC
  CCTGTAAAGGAGGAAGCTGTGGCAGAGGTGGTCACCATCACCAAATCGGTAAAGGTGCAC
  TTGGAGAAAGAGACCAAAGAAGAGGGGAAGCCACTGCAGCAGGAGAAAGAGAAGGAGAAA
  GCGGGAGGAGAGGGAGGAAGTGAGGAGGAAGGGAGTGATAAAGGTGCCAAGGGATCCAGG
  AAGGAAGACATAGCTGTCAATGGGGAGGTAGAAGGAAAAGAGGAGGTAGAGCAGGAGACC
  AAGGAAAAAGGCAGTGGGAGGGAAGAGGAGAAAGGCGTTGTCACCAATGGCCTAGACTTG
  AGCCCAGCAGATGAAAAGAAGGGGGGTGATAAAAGTGAGGAGAAAGTGGTGGTGACCAAA
  ACGGTAGAAAAAATCACCAGTGAGGGGGGAGATGGTGCTACCAAATACATCACTAAATCT
  GTAACCGTCACTCAAAAGGTTGAAGAGCATGAAGAGACCTTTGAGGAGAAACTAGTGTCT
  ACTAAAAAGGTAGAAAAAGTCACTTCACACGCCATAGTAAAGGAAGTCACCCAGAGTGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001105541
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105541.1, NP_001099011.1
• RefSeq Size: 2514 bp
• RefSeq ORF: 1623 bp
• Locus ID: 4741
• Cytogenetics: 8p21.2
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1.