ZIC2 (NM_007129) Human Tagged ORF Clone

CAT#: RC220798

ZIC2 (Myc-DDK-tagged)-Human Zic family member 2 (ZIC2)

Product Images

Circular map for RC220798

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  Western blot validation of overexpression lysate (Cat# LY416175) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220798 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZIC2 protein (Cat# TP320798). The protein was produced from HEK293T cells transfected with ZIC2 cDNA clone (Cat# RC220798) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZIC2
• Synonyms: HPE5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220798 representing NM_007129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTGGACGCGGGTCCGCAGTTCCCGGCCATCGGGGTGGGCAGCTTCGCGCGCCACCATCACCACT
  CCGCCGCGGCGGCGGCGGCGGCTGCCGCCGAGATGCAGGACCGTGAACTGAGCCTGGCGGCGGCGCAGAA
  CGGCTTCGTTGACTCCGCCGCCGCGCACATGGGAGCCTTCAAGCTCAACCCGGGCGCGCACGAGCTGTCC
  CCGGGCCAGAGCTCGGCGTTCACGTCGCAGGGCCCCGGCGCCTACCCCGGCTCCGCTGCGGCTGCCGCTG
  CGGCCGCAGCGCTCGGGCCCCACGCCGCGCACGTTGGCTCCTACTCTGGGCCGCCCTTCAACTCCACCCG
  GGACTTCCTGTTCCGCAGCCGCGGCTTCGGGGACTCGGCGCCGGGCGGCGGGCAGCACGGGCTGTTCGGG
  CCGGGCGCGGGCGGCCTGCACCACGCGCACTCGGACGCGCAGGGCCACCTCCTCTTCCCGGGCCTGCCAG
  AGCAGCACGGGCCGCACGGCTCGCAGAATGTGCTCAACGGGCAGATGCGCCTCGGGCTGCCCGGCGAGGT
  GTTCGGGCGCTCGGAGCAATACCGCCAGGTGGCCAGCCCGCGGACCGACCCCTACTCGGCGGCGCAACTC
  CACAACCAGTACGGCCCCATGAATATGAACATGGGTATGAACATGGCAGCAGCCGCGGCCCACCACCACC
  ACCACCACCACCACCACCCCGGTGCCTTTTTCCGCTATATGCGGCAGCAGTGCATCAAGCAGGAGCTAAT
  CTGCAAGTGGATCGACCCCGAGCAACTGAGCAATCCCAAGAAGAGCTGCAACAAAACTTTCAGCACCATG
  CACGAGCTGGTGACACACGTCTCGGTGGAGCACGTCGGCGGCCCGGAGCAGAGCAACCACGTCTGCTTCT
  GGGAGGAGTGTCCGCGCGAGGGCAAGCCCTTCAAGGCCAAATACAAACTGGTCAACCACATCCGCGTGCA
  CACAGGCGAGAAACCCTTCCCCTGCCCCTTCCCGGGCTGTGGCAAAGTCTTCGCGCGCTCCGAGAACCTC
  AAGATCCACAAAAGGACCCACACAGGGGAGAAGCCGTTCCAGTGTGAGTTTGAGGGCTGCGACCGGCGCT
  TCGCCAACAGCAGCGACAGGAAGAAGCACATGCACGTCCACACCTCCGATAAGCCCTATCTCTGCAAGAT
  GTGCGACAAGTCCTACACGCACCCCAGCTCGCTGCGGAAGCACATGAAGGTCCATGAGTCCTCCCCGCAG
  GGCTCTGAATCCTCCCCGGCCGCCAGCTCCGGCTATGAGTCGTCCACGCCCCCGGGGCTGGTGTCCCCCA
  GCGCCGAGCCCCAGAGCAGCTCCAACCTGTCCCCAGCGGCGGCGGCAGCGGCGGCGGCGGCTGCGGCGGC
  GGCGGCCGCGGTGTCCGCGGTGCACCGGGGCGGAGGCTCGGGCAGTGGCGGCGCGGGAGGCGGCTCAGGC
  GGCGGCAGCGGCAGTGGCGGGGGCGGCGGCGGGGCGGGCGGCGGGGGCGGCGGCAGCTCTGGCGGGGGCA
  GCGGGACAGCCGGGGGTCACAGCGGCCTCTCCTCCAACTTCAATGAATGGTACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220798 representing NM_007129
  Red=Cloning site Green=Tags(s)
  
  MLLDAGPQFPAIGVGSFARHHHHSAAAAAAAAAEMQDRELSLAAAQNGFVDSAAAHMGAFKLNPGAHELS
  PGQSSAFTSQGPGAYPGSAAAAAAAAALGPHAAHVGSYSGPPFNSTRDFLFRSRGFGDSAPGGGQHGLFG
  PGAGGLHHAHSDAQGHLLFPGLPEQHGPHGSQNVLNGQMRLGLPGEVFGRSEQYRQVASPRTDPYSAAQL
  HNQYGPMNMNMGMNMAAAAAHHHHHHHHHPGAFFRYMRQQCIKQELICKWIDPEQLSNPKKSCNKTFSTM
  HELVTHVSVEHVGGPEQSNHVCFWEECPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENL
  KIHKRTHTGEKPFQCEFEGCDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSPQ
  GSESSPAASSGYESSTPPGLVSPSAEPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGGGSGSGGAGGGSG
  GGSGSGGGGGGAGGGGGGSSGGGSGTAGGHSGLSSNFNEWYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007129
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007129.5
• RefSeq Size: 2698 bp
• RefSeq ORF: 1599 bp
• Locus ID: 7546
• UniProt ID: O95409
• Cytogenetics: 13q32.3
• Domains: zf-C2H2
• Protein Families: Druggable Genome
• Protein Pathways: Hedgehog signaling pathway
• MW: 54.8 kDa
• Gene Summary: This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Expansion of an alanine repeat in the C-terminus of the encoded protein and other mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 5, a related family member on chromosome 13. [provided by RefSeq, Jul 2016]