CDYL (NM_170752) Human Tagged ORF Clone

CAT#: RC217438

CDYL (Myc-DDK-tagged)-Human chromodomain protein, Y-like (CDYL), transcript variant 3

Product Images

Circular map for RC217438

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  Western blot validation of overexpression lysate (Cat# LY406869) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217438 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDYL protein (Cat# TP317438). The protein was produced from HEK293T cells transfected with CDYL cDNA clone (Cat# RC217438) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDYL
• Synonyms: CDYL1, MGC131936, DKFZp586C1622
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217438 representing NM_170752
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCATTAACAGCCAATGGGACAACCAACATACAGACATCTGTTACAGGAGTGACTGCCAGCAAAA
  GGAAATTTATTGACGACAGAAGAGACCAGCCTTTTGACAAGCGATTGCGTTTCAGCGTGAGGCAAACAGA
  AAGTGCCTACAGATACAGAGATATTGTGGTCAGGAAGCAGGATGGCTTCACCCACATCTTGTTATCCACA
  AAGTCCTCAGAGAATAACTCACTAAATCCAGAGGTAATGAGAGAAGTCCAGAGTGCTCTGAGCACGGCCG
  CTGCCGATGACAGCAAGCTGGTACTGCTCAGCGCCGTTGGCAGCGTCTTCTGTTGTGGACTTGACTTTAT
  TTATTTTATACGACGTCTGACAGATGACAGGAAAAGAGAAAGCACTAAAATGGCAGAAGCTATCAGAAAC
  TTCGTGAATACTTTCATTCAATTTAAGAAGCCCATTATTGTAGCAGTCAATGGCCCAGCCATTGGTCTAG
  GAGCATCTATATTGCCTCTTTGCGATGTGGTTTGGGCTAATGAAAAGGCTTGGTTTCAAACACCCTATAC
  CACCTTCGGACAGAGTCCAGATGGCTGTTCTACCGTTATGTTTCCCAAGATAATGGGAGGAGCATCTGCA
  AACGAGATGCTGCTCAGTGGACGGAAGCTGACAGCGCAGGAGGCGTGTGGCAAGGGCCTGGTCTCCCAGG
  TGTTTTGGCCCGGGACGTTCACTCAGGAAGTGATGGTTCGCATTAAGGAGCTTGCCTCGTGCAATCCAGT
  TGTGCTTGAGGAATCCAAAGCCCTCGTGCGCTGCAACATGAAGATGGAGCTGGAGCAGGCCAACGAGAGG
  GAGTGTGAGGTGCTGAAGAAAATCTGGGGCTCGGCCCAGGGGACGGACTCCATGTTAAAGTACATGCAGA
  GGAAGATCGATGAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217438 representing NM_170752
  Red=Cloning site Green=Tags(s)
  
  MDALTANGTTNIQTSVTGVTASKRKFIDDRRDQPFDKRLRFSVRQTESAYRYRDIVVRKQDGFTHILLST
  KSSENNSLNPEVMREVQSALSTAAADDSKLVLLSAVGSVFCCGLDFIYFIRRLTDDRKRESTKMAEAIRN
  FVNTFIQFKKPIIVAVNGPAIGLGASILPLCDVVWANEKAWFQTPYTTFGQSPDGCSTVMFPKIMGGASA
  NEMLLSGRKLTAQEACGKGLVSQVFWPGTFTQEVMVRIKELASCNPVVLEESKALVRCNMKMELEQANER
  ECEVLKKIWGSAQGTDSMLKYMQRKIDEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170752
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170752.1, NP_736608.1
• RefSeq Size: 2805 bp
• RefSeq ORF: 929 bp
• Locus ID: 9425
• Cytogenetics: 6p25.1
• MW: 34.4 kDa
• Gene Summary: Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]