Stk36 (BC043103) Mouse Tagged ORF Clone

CAT#: MR211549

Stk36 (Myc-DDK-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete

Product Images

Circular map for MR211549

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stk36
• Synonyms: FU, MGC58023, B930045J24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211549 representing BC043103
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAGTACCACGTTTTGGAGATGATTGGAGAAGGCTCTTTTGGGAGAGTGTATAAGGGCCGAAAAA
  AATACAGTGCTCAGGTGGTGGCCTTGAAGTTCATCCCCAAACTGGGGCGCTCAGAGAAAGAGCTGAGGAA
  TCTGCAACGAGAGATTGAAATCATGCGGGGTCTGTGGCATCCCAACATTGTGCATATGCTCGACAGCTTT
  GAGACTGACAAAGAGGTGGTGGTGGTGACAGACTACGCTGAAGGAGAACTCTTTCAGATTCTGGAAGATG
  ATGGAAAACTTCCTGAAGACCAGGTTCAGGCCATCGCTGCCCAGTTGGTGTCAGCTCTGTACTACCTGCA
  TTCCCACCGCATCCTACACCGCGACATGAAACCGCAGAACATTCTTCTCGCCAAGGGTGGTGGCATTAAA
  CTTTGTGACTTTGGATTCGCCCGAGCTATGAGCACCAACACCATGGTGCTGACGTCGATCAAAGGCACAC
  CGCTCTATATGTCTCCAGAGCTGGTGGAGGAGCGACCATATGACCACACCGCAGACCTCTGGTCTGTGGG
  CTGCATCCTGTATGAGCTGGCTGTCGGCACGCCTCCCTTCTACACCACCAGCATCTTTCAGCTGGTTAGC
  CTCATTCTCAAGGACCCTGTGCGCTGGCCCTCCACCATTAGTTCCTGCTTCAAGAACTTCTTGCAGGGGC
  TGCTCACCAAGGACCCCCGGCAGCGTCTGTCCTGGCCAGACCTCTTACATCACCCCTTTATTGCCGGCCG
  TGTCACCATCATAACTGAACCAGCAGGCTCCGATTTGGGCACCCCATTTACTAGTCGCCTACCCCCAGAA
  CTTCAGGTCCTCAAGGATGAACAGGCGCATCGGCTCGCACCCAAGGGTAACCAGTCTCGCATCCTGCGCC
  AGGCCTGTAAACTCATGGCTGAAGAAGCCAAGCAGAAGGAAGACCAAAATGCAGGATCTGCCCTTGAACA
  AGAAGACGGGCTCTGCAAGGTGACACCCAGCACAGCCCCCGTGCCTGGACTAAAGGCCACTCCTCAGGAG
  TCAAGCCTCTTGGCTGGTATACTGGCTTCAGAAATGAAGAACAACTGGGAAGACTGGGGGGCTGGAGAAG
  CACCCCGTACCTCTCGGGAAAACCACATCAACCTGGAGTGTGAACAAGGCTTCCCAGAGCCGAGGCCAGA
  GGCGATGGGCCGGCAGAGCACTGATGTAGTGGATCCTGAAAATGAGGAGCCAGACAGTGATGATGAGTGG
  CAACGCCTACTAGAGACCAGCGAGCCTGGGCCTGTGCAGCTGAAGTCCCCCCTCACCCTGTTGTGTAACC
  CTGACTTCTGCCAGCGCATCCAGAGTCAGCTGCGCGGGACTGGCGAGCAGATCCTGAAAGGCGTTCTGGA
  TGGTGTGTCCCACCTCCTTCCTGTACTCCGCATCCTGAGTAGTCTCCTATCCAGCTGCAATGACTCTGTG
  CTCTTGTATTCCTTCTGCCAGGAGGCAGGACTGCCTGAGCTGCCTCTCAGCCTTCTTAGGTACAGCCAGG
  AGAGTAGCAGCATCCAGCAGCAACCTTGGTATGGGGCGCTCTTACGGGACCTGGTGGCTGTGGTTCAGGC
  CTACTTTTCGTGCACCTTCAATCTGGAGAGGAGCCAGACAGGTGACAGCCTACAGGTGTTTCAGGAGGCC
  GCCAGCCTCTTTTTGGACCTGTTGGGGAAGCTGCTGGCCCAATCAGATGATTCCGAGCAGACATTTCGAA
  GGGATAGCCTTATGTGCTTTGCTGTCTTGTGTGAAGCTGTGGACGGAAACAGCTGGGCCGTCTCCAAAGC
  CTTCTACTCCAGCCTGTTGACCACACAGCGCGCTGTGTTGGATGGCCTCCTTCACGGCCTGACAGTCCCA
  CAGCTTCCTTTCCACACACCACCAGGAGCCCCACAAGTGAGCCAGCCGCTGCGGGAGCAGAGTGAAGATG
  TACCTGGAGCCATCTCTTCTGCCCTGGCAGCCATGTGTACTGCTCCTGTGGGGCTGCCCAGCTGTTGGGA
  TGCCAAGGAGCAGGTCTCTTGGCATTTGGCCAACCAGCTAACTGAAGACAGCAGCCAACTGAGGCCATCC
  CTCATCTCTGGTCTGCGACACCATGTCCTGTGCCTCCACCTTCTCAAGGTTCTCTACGCCTGCTGCTATA
  TTAGTGAGCGCCTGTGCCATATTCTGGGGCAAGAGCCCCTGGCCTTGGAGTCGCTGCTGATGTTAGTCCA
  GGGCAAGGTGAAAGTAGCAGATTGGGAAGAGTCCACTGAGGTGGCACTTTACCTCCTGTCCCTTCTTGTC
  TTTCGGCTCCAAGATCTGCCTTCAGGGATGGAGAAGCTGGGCAGTGAGGTTGCTACTCTCTTTACCCACT
  CACATGTCGTCTCTCTTGTGAATGCGGCAGCCTGTCTCTTAGGACAGCTTGGTCAGCAAGGGGTGACCTT
  TGACCTCCAGCCCCGGGAATGGATTGCTGCAGCTGCACATGCCTTGTCTGCCCCTGCAGAGGTCCGGCTG
  ACTCCGCCGTATAGTTGTGGCTTCTATGATGGCCTCCTCATTCTCCTGCTTCAGCTTCTTATGCAGGGGA
  AGCCTGGCCTGATCAGGGATGTGGTTGGTTCAGAGGTGTGGACCATTCTGTGGCACCGCTTTTCCATGGC
  CCTGAGGCTGCCCGAGGAGGTGTCTGCCCAGGAAGACGACCTGCTACTATCAAGTCCCTCCAGCCTAGAG
  CCAGACTGGACACTGATTTCACCCCAAGGCATGGCAGCCTTGCTGAGCCTGGCCATGGCCATCTTCACCC
  AGGAGTCCCAGTTATGCCTGAGCCACCTGTCCCAGCATGGCAGTGTCCTCATGCTGACCCTGAAGCACCT
  GCTTTCACCCAGCTTCTTGCACCACCTGAGCCAGGCGCCGCAGGGGCCCGAGTTTCTCCCCGTTGTGGTG
  CTCTCCGTGTGCAAGCTGCTCTGCTTCCCTTTTGCCCTGGATGTGGATGCTGACCTCCTTGTAGGTGTCT
  TGGCTGACCTCAGGGCCTCGGAAGTGGTAGTCTGCCTGCTGCAGGTCTGCTGCCACCACCTTTCGTTGTT
  ACAAGCAGAGCTGCCCATTGGCCTCCTTACACGCCTGGCCCTCACGGATTCTGCCTCTCTCAGCGGCCGC
  TCGCTACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211549 representing BC043103
  Red=Cloning site Green=Tags(s)
  
  MEKYHVLEMIGEGSFGRVYKGRKKYSAQVVALKFIPKLGRSEKELRNLQREIEIMRGLWHPNIVHMLDSF
  ETDKEVVVVTDYAEGELFQILEDDGKLPEDQVQAIAAQLVSALYYLHSHRILHRDMKPQNILLAKGGGIK
  LCDFGFARAMSTNTMVLTSIKGTPLYMSPELVEERPYDHTADLWSVGCILYELAVGTPPFYTTSIFQLVS
  LILKDPVRWPSTISSCFKNFLQGLLTKDPRQRLSWPDLLHHPFIAGRVTIITEPAGSDLGTPFTSRLPPE
  LQVLKDEQAHRLAPKGNQSRILRQACKLMAEEAKQKEDQNAGSALEQEDGLCKVTPSTAPVPGLKATPQE
  SSLLAGILASEMKNNWEDWGAGEAPRTSRENHINLECEQGFPEPRPEAMGRQSTDVVDPENEEPDSDDEW
  QRLLETSEPGPVQLKSPLTLLCNPDFCQRIQSQLRGTGEQILKGVLDGVSHLLPVLRILSSLLSSCNDSV
  LLYSFCQEAGLPELPLSLLRYSQESSSIQQQPWYGALLRDLVAVVQAYFSCTFNLERSQTGDSLQVFQEA
  ASLFLDLLGKLLAQSDDSEQTFRRDSLMCFAVLCEAVDGNSWAVSKAFYSSLLTTQRAVLDGLLHGLTVP
  QLPFHTPPGAPQVSQPLREQSEDVPGAISSALAAMCTAPVGLPSCWDAKEQVSWHLANQLTEDSSQLRPS
  LISGLRHHVLCLHLLKVLYACCYISERLCHILGQEPLALESLLMLVQGKVKVADWEESTEVALYLLSLLV
  FRLQDLPSGMEKLGSEVATLFTHSHVVSLVNAAACLLGQLGQQGVTFDLQPREWIAAAAHALSAPAEVRL
  TPPYSCGFYDGLLILLLQLLMQGKPGLIRDVVGSEVWTILWHRFSMALRLPEEVSAQEDDLLLSSPSSLE
  PDWTLISPQGMAALLSLAMAIFTQESQLCLSHLSQHGSVLMLTLKHLLSPSFLHHLSQAPQGPEFLPVVV
  LSVCKLLCFPFALDVDADLLVGVLADLRASEVVVCLLQVCCHHLSLLQAELPIGLLTRLALTDSASLSGR
  SLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC043103
• ORF Size: 3159 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC043103.1
• RefSeq Size: 3378 bp
• RefSeq ORF: 3161 bp
• Locus ID: 269209
• Cytogenetics: 1 C4
• MW: 116.2 kDa
• Gene Summary: Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur. Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function]