Msh2 (NM_008628) Mouse Tagged ORF Clone

CAT#: MR211249

Msh2 (Myc-DDK-tagged) - Mouse mutS homolog 2 (E. coli) (Msh2)

Product Images

Circular map for MR211249

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Msh2
• Synonyms: AI788990
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211249 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGCAGCCTAAGGAGACGCTGCAGTTGGAAGGCGCGGCCGAGGCGGGCTTCGTGCGCTTCTTTG
  AGGGCATGCCGGAGAAGCCGAGCACCACGGTGCGCCTCTTCGACCGCGGCGACTTTTACACGGCGCACGG
  AGAGGACGCGCTGCTGGCGGCCCGCGAGGTGTTCAAGACCCAGGGCGTGATCAAGTACATGGGGCCGGCA
  GGGAGTAAGACCCTGCAGAGTGTTGTGCTTAGCAAGATGAACTTTGAGTCTTTCGTGAAAGATCTTCTTC
  TGGTTCGCCAGTATCGAGTTGAAGTTTATAAGAATAAAGCTGGAAATAAGGCGTCTAAGGAGAATGAGTG
  GTATCTGGCATTTAAGGCTTCTCCCGGCAATCTTTCTCAGTTTGAAGACATCCTGTTTGGTAACAATGAC
  ATGTCAGCTTCCGTTGGCGTTATGGGTATTAAAATGGCCGTGGTTGATGGTCAAAGACATGTTGGAGTTG
  GGTATGTGGATTCCACCCAGAGGAAGCTAGGCTTGTGTGAGTTCCCCGAGAATGATCAGTTCTCCAATCT
  CGAGGCTCTTCTGATTCAGATTGGACCAAAGGAATGCGTTTTACCAGGAGGAGAGACTACTGGAGACATG
  GGGAAACTGAGGCAGGTTATCCAGAGAGGAGGGATTCTGATCACAGAAAGAAAGAGAGCCGACTTTTCCA
  CTAAAGACATTTATCAGGATCTCAACCGGTTACTGAAAGGCAAAAAAGGAGAACAGATAAATAGTGCTGC
  CCTACCAGAGATGGAGAATCAGGTTGCAGTTTCATCACTATCTGCAGTAATCAAGTTTTTAGAACTCTTA
  TCAGACGATTCAAATTTTGGGCAGTTTGAACTGGCCACTTTTGACTTCAGCCAGTACATGAAGTTGGACA
  TGGCAGCAGTTAGAGCCCTCAACCTTTTCCAGGGTTCTGTTGAAGACACCACTGGCTCTCAGTCTCTGGC
  CGCATTATTGAATAAATGCAAAACTGCTCAAGGACAAAGATTGGTTAACCAGTGGATCAAGCAGCCGCTC
  ATGGATAGGAACAGGATAGAGGAGAGGTTAAATTTAGTGGAAGCTTTTGTCGAGGATTCAGAACTGAGGC
  AGAGTTTACAGGAGGATTTGCTTCGCCGGTTTCCAGACCTTAACCGGCTTGCCAAGAAATTCCAGAGACA
  AGCAGCGAATTTACAAGACTGTTACCGACTGTATCAGGGTATTAACCAGCTCCCCAGCGTCATCCAGGCT
  CTGGAGAAATACGAAGGAAGACACCAGGCACTGTTGTTGGCAGTTTTTGTGACTCCTCTTATTGATCTTC
  GTTCTGATTTTTCAAAATTTCAAGAAATGATAGAAACAACTTTAGATATGGATCAGGTGGAAAACCACGA
  GTTCCTTGTAAAACCTTCATTTGATCCTAACCTGAGTGAACTAAGAGAAGTCATGGATGGCCTGGAGAAG
  AAGATGCAGTCCACCTTAATAAATGCAGCCCGGGGGCTCGGATTGGATCCTGGCAAACAGATTAAATTGG
  ACTCCAGTGCACAGTTTGGATATTATTTCCGTGTAACCTGCAAGGAAGAGAAAGTGCTTCGCAACAACAA
  GAACTTCAGCACAGTGGACATCCAGAAGAATGGCGTGAAGTTTACCAACAGTGAATTGTCCTCTTTAAAT
  GAAGAATATACTAAGAACAAAGGCGAGTATGAAGAGGCCCAGGATGCCATTGTTAAAGAAATTGTCAATA
  TTTCCTCAGGCTACGTAGAGCCAATGCAGACGCTCAACGATGTGCTGGCTCACTTAGACGCCATTGTTAG
  CTTCGCTCATGTGTCAAACGCAGCACCCGTTCCTTATGTACGACCAGTCATCTTGGAGAAAGGAAAAGGG
  AGAATTATATTGAAAGCCTCCAGGCATGCTTGTGTTGAAGTTCAAGATGAAGTTGCATTTATTCCAAATG
  ACGTGCACTTTGAAAAAGATAAACAGATGTTCCACATCATTACTGGTCCCAATATGGGAGGTAAATCAAC
  ATACATTCGTCAGACCGGGGTGATTGTACTCATGGCCCAAATCGGGTGTTTTGTGCCCTGTGAGTCGGCA
  GAAGTGTCCATTGTGGATTGCATCCTTGCTCGAGTCGGGGCTGGTGACAGTCAACTGAAAGGCGTCTCCA
  CATTCATGGCTGAAATGCTGGAGACTGCTTCCATCCTCAGGTCAGCAACCAAAGACTCCTTAATAATCAT
  TGATGAGCTGGGAAGAGGAACCTCTACCTATGATGGATTTGGGTTAGCATGGGCTATATCAGATTACATT
  GCAACGAAGATTGGTGCCTTTTGCATGTTTGCCACCCATTTTCATGAACTTACTGCTTTGGCCAACCAAA
  TACCAACTGTTAATAATCTACATGTCACAGCGCTCACTACTGAGGAGACCCTAACTATGCTTTACCAAGT
  GAAAAAAGGTGTCTGTGATCAGAGTTTCGGGATTCACGTGGCTGAGCTCGCTAACTTCCCGAGGCACGTG
  ATAGCGTGCGCCAAGCAGAAGGCTCTAGAGCTTGAAGAATTTCAGAACATTGGAACCTCGCTGGGATGTG
  ACGAAGCCGAGCCGGCTGCAAAGAGACGCTGCCTGGAAAGAGAGCAAGGTGAGAAAATTATTCTGGAGTT
  CCTGTCGAAGTTCAAGCAGGTGCCCTTTACTGCCATGTCGGAGGAGAGCATCTCCGCGAAGCTGAAGCAA
  CTGAAAGCCGAGGTGGTCGCAAAGAACAACAGCTTCGTAAACGAGATCATTTCACGGATAAAGGCTCCGG
  CTCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211249 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVQPKETLQLEGAAEAGFVRFFEGMPEKPSTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPA
  GSKTLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNKAGNKASKENEWYLAFKASPGNLSQFEDILFGNND
  MSASVGVMGIKMAVVDGQRHVGVGYVDSTQRKLGLCEFPENDQFSNLEALLIQIGPKECVLPGGETTGDM
  GKLRQVIQRGGILITERKRADFSTKDIYQDLNRLLKGKKGEQINSAALPEMENQVAVSSLSAVIKFLELL
  SDDSNFGQFELATFDFSQYMKLDMAAVRALNLFQGSVEDTTGSQSLAALLNKCKTAQGQRLVNQWIKQPL
  MDRNRIEERLNLVEAFVEDSELRQSLQEDLLRRFPDLNRLAKKFQRQAANLQDCYRLYQGINQLPSVIQA
  LEKYEGRHQALLLAVFVTPLIDLRSDFSKFQEMIETTLDMDQVENHEFLVKPSFDPNLSELREVMDGLEK
  KMQSTLINAARGLGLDPGKQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSELSSLN
  EEYTKNKGEYEEAQDAIVKEIVNISSGYVEPMQTLNDVLAHLDAIVSFAHVSNAAPVPYVRPVILEKGKG
  RIILKASRHACVEVQDEVAFIPNDVHFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESA
  EVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISDYI
  ATKIGAFCMFATHFHELTALANQIPTVNNLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPRHV
  IACAKQKALELEEFQNIGTSLGCDEAEPAAKRRCLEREQGEKIILEFLSKFKQVPFTAMSEESISAKLKQ
  LKAEVVAKNNSFVNEIISRIKAPAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008628
• ORF Size: 2808 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008628.1, NM_008628.2, NP_032654.1
• RefSeq Size: 3056 bp
• RefSeq ORF: 2808 bp
• Locus ID: 17685
• UniProt ID: P43247
• Cytogenetics: 17 57.87 cM
• MW: 104.2 kDa
• Gene Summary: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.[UniProtKB/Swiss-Prot Function]