Msh2 (NM_008628) Mouse Untagged Clone

CAT#: MC222681

Msh2 (untagged) - Mouse mutS homolog 2 (E. coli) (Msh2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Msh2
• Synonyms: AI788990
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222681 representing NM_008628
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGCAGCCTAAGGAGACGCTGCAGTTGGAAGGCGCGGCCGAGGCGGGCTTCGTGCGCTTCTTTG
  AGGGCATGCCGGAGAAGCCGAGCACCACGGTGCGCCTCTTCGACCGCGGCGACTTTTACACGGCGCACGG
  AGAGGACGCGCTGCTGGCGGCCCGCGAGGTGTTCAAGACCCAGGGCGTGATCAAGTACATGGGGCCGGCA
  GGGAGTAAGACCCTGCAGAGTGTTGTGCTTAGCAAGATGAACTTTGAGTCTTTCGTGAAAGATCTTCTTC
  TGGTTCGCCAGTATCGAGTTGAAGTTTATAAGAATAAAGCTGGAAATAAGGCGTCTAAGGAGAATGAGTG
  GTATCTGGCATTTAAGGCTTCTCCCGGCAATCTTTCTCAGTTTGAAGACATCCTGTTTGGTAACAATGAC
  ATGTCAGCTTCCGTTGGCGTTATGGGTATTAAAATGGCCGTGGTTGATGGTCAAAGACATGTTGGAGTTG
  GGTATGTGGATTCCACCCAGAGGAAGCTAGGCTTGTGTGAGTTCCCCGAGAATGATCAGTTCTCCAATCT
  CGAGGCTCTTCTGATTCAGATTGGACCAAAGGAATGCGTTTTACCAGGAGGAGAGACTACTGGAGACATG
  GGGAAACTGAGGCAGGTTATCCAGAGAGGAGGGATTCTGATCACAGAAAGAAAGAGAGCCGACTTTTCCA
  CTAAAGACATTTATCAGGATCTCAACCGGTTACTGAAAGGCAAAAAAGGAGAACAGATAAATAGTGCTGC
  CCTACCAGAGATGGAGAATCAGGTTGCAGTTTCATCACTATCTGCAGTAATCAAGTTTTTAGAACTCTTA
  TCAGACGATTCAAATTTTGGGCAGTTTGAACTGGCCACTTTTGACTTCAGCCAGTACATGAAGTTGGACA
  TGGCAGCAGTTAGAGCCCTCAACCTTTTCCAGGGTTCTGTTGAAGACACCACTGGCTCTCAGTCTCTGGC
  CGCATTATTGAATAAATGCAAAACTGCTCAAGGACAAAGATTGGTTAACCAGTGGATCAAGCAGCCGCTC
  ATGGATAGGAACAGGATAGAGGAGAGGTTAAATTTAGTGGAAGCTTTTGTCGAGGATTCAGAACTGAGGC
  AGAGTTTACAGGAGGATTTGCTTCGCCGGTTTCCAGACCTTAACCGGCTTGCCAAGAAATTCCAGAGACA
  AGCAGCGAATTTACAAGACTGTTACCGACTGTATCAGGGTATTAACCAGCTCCCCAGCGTCATCCAGGCT
  CTGGAGAAATACGAAGGAAGACACCAGGCACTGTTGTTGGCAGTTTTTGTGACTCCTCTTATTGATCTTC
  GTTCTGATTTTTCAAAATTTCAAGAAATGATAGAAACAACTTTAGATATGGATCAGGTGGAAAACCACGA
  GTTCCTTGTAAAACCTTCATTTGATCCTAACCTGAGTGAACTAAGAGAAGTCATGGATGGCCTGGAGAAG
  AAGATGCAGTCCACCTTAATAAATGCAGCCCGGGGGCTCGGATTGGATCCTGGCAAACAGATTAAATTGG
  ACTCCAGTGCACAGTTTGGATATTATTTCCGTGTAACCTGCAAGGAAGAGAAAGTGCTTCGCAACAACAA
  GAACTTCAGCACAGTGGACATCCAGAAGAATGGCGTGAAGTTTACCAACAGTGAATTGTCCTCTTTAAAT
  GAAGAATATACTAAGAACAAAGGCGAGTATGAAGAGGCCCAGGATGCCATTGTTAAAGAAATTGTCAATA
  TTTCCTCAGGCTACGTAGAGCCAATGCAGACGCTCAACGATGTGCTGGCTCACTTAGACGCCATTGTTAG
  CTTCGCTCATGTGTCAAACGCAGCACCCGTTCCTTATGTACGACCAGTCATCTTGGAGAAAGGAAAAGGG
  AGAATTATATTGAAAGCCTCCAGGCATGCTTGTGTTGAAGTTCAAGATGAAGTTGCATTTATTCCAAATG
  ACGTGCACTTTGAAAAAGATAAACAGATGTTCCACATCATTACTGGTCCCAATATGGGAGGTAAATCAAC
  ATACATTCGTCAGACCGGGGTGATTGTACTCATGGCCCAAATCGGGTGTTTTGTGCCCTGTGAGTCGGCA
  GAAGTGTCCATTGTGGATTGCATCCTTGCTCGAGTCGGGGCTGGTGACAGTCAACTGAAAGGCGTCTCCA
  CATTCATGGCTGAAATGCTGGAGACTGCTTCCATCCTCAGGTCAGCAACCAAAGACTCCTTAATAATCAT
  TGATGAGCTGGGAAGAGGAACCTCTACCTATGATGGATTTGGGTTAGCATGGGCTATATCAGATTACATT
  GCAACGAAGATTGGTGCCTTTTGCATGTTTGCCACCCATTTTCATGAACTTACTGCTTTGGCCAACCAAA
  TACCAACTGTTAATAATCTACATGTCACAGCGCTCACTACTGAGGAGACCCTAACTATGCTTTACCAAGT
  GAAAAAAGGTGTCTGTGATCAGAGTTTCGGGATTCACGTGGCTGAGCTCGCTAACTTCCCGAGGCACGTG
  ATAGCGTGCGCCAAGCAGAAGGCTCTAGAGCTTGAAGAATTTCAGAACATTGGAACCTCGCTGGGATGTG
  ACGAAGCCGAGCCGGCTGCAAAGAGACGCTGCCTGGAAAGAGAGCAAGGTGAGAAAATTATTCTGGAGTT
  CCTGTCGAAGGTCAAGCAGGTGCCCTTTACTGCCATGTCGGAGGAGAGCATCTCCGCGAAGCTGAAGCAA
  CTGAAAGCCGAGGTGGTCGCAAAGAACAACAGCTTCGTAAACGAGATCATTTCACGGATAAAGGCTCCGG
  CTCCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008628
• Insert Size: 2808 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008628.2, NP_032654.1
• RefSeq Size: 3056 bp
• RefSeq ORF: 2808 bp
• Locus ID: 17685
• UniProt ID: P43247
• Cytogenetics: 17 57.87 cM
• Gene Summary: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.[UniProtKB/Swiss-Prot Function]