Ercc1 (NM_001127324) Mouse Tagged ORF Clone

CAT#: MG224948

Ercc1 (tGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency complementation group 1 (Ercc1) transcript variant 2, (10ug)

Product Images

Circular map for MG224948

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ercc1
• Synonyms: Ercc-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224948 representing NM_001127324
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCTGGGAAGGACGAGGAAAGTCGGCCACAGCCCTCAGGACCACCCACCAGGAGGAAGTTTGTTA
  TCCCACTGGAGGAAGAAGAGGTGCCCTGTGCAGGGGTCAAGCCCTTATTCAGATCGTCACGGAATCCCAC
  CATCCCAGCAACCTCAGCCCACGTGGCCCCTCAGACGTATGCTGAGTACGCCATCACCCAGCCTCCAGGA
  GGGGCTGGGGCCACAGTGCCCACAGGCTCTGAACCTGCGGCAGGAGAGAACCCCAGCCAGACCCTGAAAA
  CAGGAGCAAAGTCTAATAGCATCATCGTGAGCCCGAGGCAGAGGGGCAACCCCGTGTTGAAGTTTGTGCG
  CAATGTGCCCTGGGAATTCGGTGAGGTGATTCCCGATTATGTGCTGGGCCAGAGCACCTGCGCCCTTTTC
  CTCAGCCTCCGCTACCACAACCTCCATCCAGACTACATCCATGAACGGCTGCAGAGCCTGGGGAAGAACT
  TCGCCCTTCGTGTGCTGCTGGTTCAAGTGGATGTGAAAGATCCCCAGCAGGCTCTCAAGGAGCTGGCTAA
  GATGTGCATCTTGGCTGACTGCACCCTGGTCCTGGCCTGGAGTGCAGAGGAAGCAGGGCGGTACCTGGAG
  ACCTACAAGGCGTATGAGCAGAAGCCAGCCGACCTCCTTATGGAAAAGCTGGAGCAGAACTTCCTATCAC
  GGGTATCACTGTGCCTCTTGTCCTCCTGTACCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224948 representing NM_001127324
  Red=Cloning site Green=Tags(s)
  
  MDPGKDEESRPQPSGPPTRRKFVIPLEEEEVPCAGVKPLFRSSRNPTIPATSAHVAPQTYAEYAITQPPG
  GAGATVPTGSEPAAGENPSQTLKTGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGEVIPDYVLGQSTCALF
  LSLRYHNLHPDYIHERLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSAEEAGRYLE
  TYKAYEQKPADLLMEKLEQNFLSRVSLCLLSSCTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127324
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127324.1, NP_001120796.1
• RefSeq Size: 1241 bp
• RefSeq ORF: 738 bp
• Locus ID: 13870
• UniProt ID: P07903
• Cytogenetics: 7 9.6 cM
• Gene Summary: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4 (By similarity).[UniProtKB/Swiss-Prot Function]