Atf1 (NM_007497) Mouse Tagged ORF Clone

CAT#: MG223254

Atf1 (tGFP-tagged) - Mouse activating transcription factor 1 (Atf1), (10ug)

Product Images

Circular map for MG223254

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atf1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223254 representing NM_007497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATTCCCACAAGAGTAACACGACAGAGACTGCATCTCAGCCTGGTTCAACCGTTGCGGGGCCTC
  ACGTTTCTCAGATAGTACATCAGGTGTCATCGTTATCAGAAAGTGAGGAGTCTCAGGACTCATCTGACAG
  CATAGGCTCCTCACAGAAAGCTCACGGGATCCTGGCCCGGCGCCCATCCTACAGGAAAATTTTGAAAGAC
  CTCTCTTCTGAAGATACACGGGGCAGAAAAGGGGAAGGAGAAAATCCCAGCATTTCTGCCATCACGTCTA
  TGTCTGTTCCAGCCCCCATCTATCAGACGAGCAGCGGACAGTACATTGCCATTGCCCCAAACGGAGCCTT
  ACATTTGGCCAGTCCAAGCACGGATGGAGTGCAGGCACTGCAGACATTAACCATGACAAATTCAAGCAGT
  ACTCAGCAGGGGACGATCCTCCAGTATGCACAGACCTCTGACGGACAGCAGATACTCGTCCCGAGCAACC
  AGGTGGTTGTACAGACTGCATCAGGAGATATGCAGACCTACCAGATCCGTACCACGCCATCTGCCACGTC
  TCTCCCACAGACCGTGGTGATGACTTCTCCTGTGACTCTTACATCTCAGACAACAAAGACAGATGACCCT
  CAACTAAGAAGAGAAATACGACTGATGAAAAACAGAGAAGCTGCCCGAGAGTGCCGCCGGAAGAAGAAGG
  AGTACGTGAAGTGCCTGGAGAACCGTGTTGCTGTTCTGGAAAATCAAAATAAAACTCTAATAGAGGAGCT
  AAAGACTTTGAAGGACCTTTATTCTCATAAAAGTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223254 representing NM_007497
  Red=Cloning site Green=Tags(s)
  
  MEDSHKSNTTETASQPGSTVAGPHVSQIVHQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKD
  LSSEDTRGRKGEGENPSISAITSMSVPAPIYQTSSGQYIAIAPNGALHLASPSTDGVQALQTLTMTNSSS
  TQQGTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDP
  QLRREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSHKSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007497
• ORF Size: 807 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007497.2
• RefSeq Size: 2329 bp
• RefSeq ORF: 810 bp
• Locus ID: 11908
• UniProt ID: P81269
• Cytogenetics: 15 F1
• Gene Summary: Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation (By similarity).[UniProtKB/Swiss-Prot Function]