Atf1 (NM_007497) Mouse Untagged Clone

CAT#: MC208158

Atf1 (untagged) - Mouse activating transcription factor 1 (Atf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208158 representing NM_007497
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATTCCCACAAGAGTAACACGACAGAGACTGCATCTCAGCCTGGTTCAACCGTTGCGGGGCCTC
  ACGTTTCTCAGATAGTACATCAGGTGTCATCGTTATCAGAAAGTGAGGAGTCTCAGGACTCATCTGACAG
  CATAGGCTCCTCACAGAAAGCTCACGGGATCCTGGCCCGGCGCCCATCCTACAGGAAAATTTTGAAAGAC
  CTCTCTTCTGAAGATACACGGGGCAGAAAAGGGGAAGGAGAAAATCCCAGCATTTCTGCCATCACGTCTA
  TGTCTGTTCCAGCCCCCATCTATCAGACGAGCAGCGGACAGTACATTGCCATTGCCCCAAACGGAGCCTT
  ACAGTTGGCCAGTCCAAGCACGGATGGAGTGCAGGCACTGCAGACATTAACCATGACAAATTCAAGCAGT
  ACTCAGCAGGGGACGATCCTCCAGTATGCACAGACCTCTGACGGACAGCAGATACTCGTCCCGAGCAACC
  AGGTGGTTGTACAGACTGCATCAGGAGATATGCAGACCTACCAGATCCGTACCACGCCGTCTGCCACGTC
  TCTCCCGCAGACCGTGGTGATGACTTCTCCTGTGACTCTTGCATCTCAGACAACAAAGACAGATGACCCT
  CAACTAAGAAGAGAAATACGACTGATGAAAAACAGAGAAGCTGCCCGAGAGTGCCGCCGGAAGAAGAAGG
  AGTACGTGAAGTGCCTGGAGAACCGTGTTGCTGTTCTGGAAAATCAAAATAAAACTCTAATAGAGGAGCT
  AAAGACTTTGAAGGACCTTTATTCTCATAAAAGTGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007497
• Insert Size: 810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007497.3, NP_031523.3
• RefSeq Size: 2329 bp
• RefSeq ORF: 810 bp
• Locus ID: 11908
• UniProt ID: P81269
• Cytogenetics: 15 F1
• Gene Summary: Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation (By similarity).[UniProtKB/Swiss-Prot Function]