Vars2 (NM_175137) Mouse Tagged ORF Clone

CAT#: MG219019

Vars2 (tGFP-tagged) - Mouse valyl-tRNA synthetase 2 mitochondrial (putative) (Vars2) nuclear gene encoding mitochondrial protein, (10ug)

Product Images

Circular map for MG219019

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vars2
• Synonyms: 1190004I24Rik; mKIAA1885; Vars2l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219019 representing NM_175137
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCATTTGCCTCTGGCCTCTTTCCGGCCACCGCTCTGGGGGCTGAGGCCCTCATGGGGTCTTTCCA
  GGCCCCAGGCACTTTGTACACAGCCGGAGCCCCACGGCTCCCCTGTCTCTCGGAGGAACCGTGAAGCCAA
  ACAGAAGCGCCTGCGCGAGAAACAGGCGGCCCTGGAGGCTGGGCTAGCTGAGAAGAGCAAGATACCTGCA
  GTGCCCACTAAGGCCTGGAGTCACAAGGAGGTAGTATTGTATGAAATCCCCACCGGGCCAGGTGAAAAGA
  AAGATGTTTCTGGGCCCCTGCCTCCTGCTTACAGTCCCCAGTATGTTGAAGCGGCCTGGTACCAGTGGTG
  GGTGCGAGAGGGCTTCTTCAAACCAGAGTATCAGGCAAGGCTGCCCCAAGCTACAGGGGAGACCTTTTCC
  ATGTGTATCCCACCTCCCAATGTCACCGGCTCCCTGCACATTGGCCATGCACTCACAGTGGCGATCCAGG
  ACGCACTTGTGCGCTGGCACCGGATGCGTGGGGATCGCGTGCTGTGGATCCCCGGGTCTGATCACGCAGG
  AATTGCTACACAGGCCATGGTGGAGAAGCAGCTATGGAAGGAGCAGAGGGTGCGGAGACATGAGCTGAGC
  CGGGAAGACTTCCTCAGGGCCGTGTGGCAGTGGAAACATGAGAAAGGAGGGGAGATCTATGAACAGCTGT
  GTGCTCTGGGTGCCTCTCTGGACTGGGATCGAGAGTGTTTCACCATGGATGCCGGCTCCTCAGCAGCTGT
  GACGGAAGCTTTCGTGCGACTCTACAACTCGGGATTGTTGTACCGGAACCGCCAGCTTGTCAACTGGTCA
  TGTACTTTGCGATCGGCCATCTCAGACATTGAGGTGGAAAGCCGGCCTCTGCCTGGCCGCACGGTGCTGC
  AGCTGCCCGGATGCCCAACCCCTGTGTCTTTTGGGCTCCTTGCTTCTGTTGCTTTCCCTGTGGATGGAGA
  GCCTGACACAGAGATTGTGGTGGGAACCACCAGGCCAGAGACCCTGCCTGGAGATGTGGCTGTGGCCGTT
  CATCCAGATGACCCGAGATACACGCATTTACATGGGCGACAGCTCCGTCATCCTTTGACAGGACAGCTTC
  TCCCCCTCATCACAGACACCACTGTTCAGCCACATGTGGGCACAGGGGCAGTGAAGGTGACTCCTGCTCA
  CAGTCCAATAGATGCTGAGATAGGGACCCGGCATGGCCTGACCCCACTGAGTGTCATTGCAGAGGATGGG
  ACCATGACATCCCTCTGTGGAGACTGGCTGCAGGGCCTTCATCGATTTGTGGCCCGGGAAAAGATCATGT
  GCACACTGAGGGAGCAGGGCCTATTCCGGGGCCTACAGGAGCACCCCATGGTACTGCCCATCTGCAGCCG
  TTCCGGGGACGTGGTGGAGTACCTGCTGAAGAGCCAGTGGTTTGTCCGCTGCCAGGAAATGGGGGACCTA
  GCTGCCAAGGCTGTGGAGTCAGGGGCTCTGGAGCTCTGGCCCTCCTTCCACCAGAAGAGCTGGCAACACT
  GGTTTGCCCACATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCATCAGATTCCAGCCTACCG
  GGTCATCGGGGAGAATGCAGAGGATGACAGGAAGGAATGTTGGGTGGTCGGACGGTCAGAGGCTGAGGCC
  AGAGCGGTAGCAGCAAAACGGACAGGGAGACCAGAGGCAGAGCTGACCCTGGAGAGGGACCCTGACGTCC
  TGGACACTTGGTTCTCTTCGGCTCTTTTCCCCTTTTCTGCCCTGGGTTGGCCGCGAGAGACGCCAGACCT
  TGCTCACTTCTACCCCCTCACCCTCTTGGAAACGGGTAGTGACCTGCTGATGTTCTGGGTGGGCCGCATG
  GTCATGCTGGGGACCCAGCTCACTGGGCAGCTCCCCTTCAGCAAGGTGCTTCTTCACTCCATGGTTCGGG
  ACAGGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAACGTGCTAGACCCACGGGACATCATTAGCGGGCA
  GGAGCTGCAGGTGCTGCAGGCCAAGCTGAGAGACGGTAACTTGGACCCTGGAGAGCTCGCCGTTGCAGCC
  GCAGCACAGAAAAAGGACTTTCCTTACGGGATCCCTGAGTGTGGGACGGATGCCCTGAGATTTGCACTGT
  GCTCCCATGGAATCCTGGGGGGCGACCTGCACCTGTCTGTCTCTGAGGTCCTGAACTACCGCCATTTCTG
  CAACAAGCTCTGGAATGCCCTGCGCTTTGTCCTCCGTGCCCTGGGGGATAACTTCGTGCCCCAACCAGCA
  GAGGAGGTAACCCCCTCCTCTCCCATGGATGCCTGGATTCTAAGCCGCCTGGCCTTTGCAGCCAGCGAAT
  GTGAGAGAGGCTTCCTGAGTCGGGAGCTTTCGCTTGTCACCCACACCCTGTATCATTTCTGGCTCCACAA
  TCTCTGTGATGTCTACCTGGAAGCAGTGAAGCCAGTGTTGTCGAGCGTGCCCCGTCCCCCAGGACCCCCT
  CAGGTCCTGTTTTCCTGTGCAGACGTGGGTCTACGCCTCCTTGCCCCGCTGATGCCCTTCCTGGCCGAGG
  AGCTGTGGCAGAGGCTGCCCCCCAGGCCAGGCGGCCCCCTTGCCCCCAGCATCTGTGTGGCCCCCTACCC
  CAGCACCCGCAGCCTGGAGTTCTGGCGCCAGCCAGAGCTGGAGCGCTGCTTCTCCAGAGTCCAAGAGGTT
  GTACAGGCATTGCGGGCGCTCCGAGCCACCTACCAGCTCACAAAAGCCCGACCCCAAGTGCTGCTGCAGT
  GCTCGGACCCAGGAGAACAAGGCCTAGTCCAGCCCTTTCTGGAGCCTCTGGGCATCCTGAGCCACTGCGG
  GGCTGTAGGATTCCTGTCCCCAGGGGCAGCAGCTCCCTCAGGGTGGGCCCTGACCCCACTGGGTGACACC
  ATGAAGATCTACATGGAGCTGCAGGGCCTGGTGGACCCCCAGAGCCAGCTGCCCCGGCTCACTGCCCGAA
  GGCAGAAGTTACAGAAGCAGCTTGATGACCTGTTGAACCGGACCATGTCAGAGGGTCTTGCAGAGAGGCA
  GCAGAGGATTTCCTCCCTCCATCTGGAATTGTCAAAGCTGGACCAGGCAGCCTCCTACCTACAGCAACTG
  ATGGATGAGGCTCCCAGTGCCAGGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219019 representing NM_175137
  Red=Cloning site Green=Tags(s)
  
  MPHLPLASFRPPLWGLRPSWGLSRPQALCTQPEPHGSPVSRRNREAKQKRLREKQAALEAGLAEKSKIPA
  VPTKAWSHKEVVLYEIPTGPGEKKDVSGPLPPAYSPQYVEAAWYQWWVREGFFKPEYQARLPQATGETFS
  MCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWIPGSDHAGIATQAMVEKQLWKEQRVRRHELS
  REDFLRAVWQWKHEKGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYNSGLLYRNRQLVNWS
  CTLRSAISDIEVESRPLPGRTVLQLPGCPTPVSFGLLASVAFPVDGEPDTEIVVGTTRPETLPGDVAVAV
  HPDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDG
  TMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRGLQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDL
  AAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAEA
  RAVAAKRTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPRETPDLAHFYPLTLLETGSDLLMFWVGRM
  VMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDPGELAVAA
  AAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFVLRALGDNFVPQPA
  EEVTPSSPMDAWILSRLAFAASECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPVLSSVPRPPGPP
  QVLFSCADVGLRLLAPLMPFLAEELWQRLPPRPGGPLAPSICVAPYPSTRSLEFWRQPELERCFSRVQEV
  VQALRALRATYQLTKARPQVLLQCSDPGEQGLVQPFLEPLGILSHCGAVGFLSPGAAAPSGWALTPLGDT
  MKIYMELQGLVDPQSQLPRLTARRQKLQKQLDDLLNRTMSEGLAERQQRISSLHLELSKLDQAASYLQQL
  MDEAPSAREL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175137
• ORF Size: 3180 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175137.4, NP_780346.3
• RefSeq Size: 4441 bp
• RefSeq ORF: 3183 bp
• Locus ID: 68915
• UniProt ID: Q3U2A8
• Cytogenetics: 17 B1