Gls2 (NM_001033264) Mouse Untagged Clone

CAT#: MC219566

Gls2 (untagged) - Mouse glutaminase 2 (liver, mitochondrial) (Gls2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gls2
• Synonyms: A330074B06Rik; AI195532; GA; GLS; Lga
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219566 representing NM_001033264
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCCATGAGGGCTCTGCAGAACGCGCTGAGCCGCGCGGGCAGCCACGGTCGGCGGGGAGGCTGGG
  GTCATCCGAGCCGGGGCCCGCTTCTGGGCAGGGGCGTCCGGTACTACCTCGGTGAGGCCGCAGCGCAGGG
  CAGGGGGACGCCGCACAGCCACCAGCCGCAGCACTCGGATCATGACGCCTCACACAGCGGCATGCTGCCT
  CGACTTGGTGACCTGCTTTTCTACACTATTGCAGAGGGACAGGAGCGTATCCCTATCCACAAGTTCACCA
  CGGCTCTGAAGGCCACTGGACTGCAGACGTCAGACCCACGGCTCCAGGACTGCATGAGCAAGATGCAGCG
  CATGGTCCAAGAGTCCAGCAGCGGTGGCCTCTTGGACCGAGAGCTCTTCCAAAAGTGTGTGAGCAGCAAC
  ATTGTGCTCCTGACTCAGGCATTCCGAAAGAAGTTTGTCATTCCTGACTTTGAGGAGTTCACGGGCCATG
  TGGATCGCATATTTGAGGATGCCAAAGAGCCCACTGGAGGCAAAGTGGCAGCCTACATCCCGCACCTGGC
  CAAATCAAACCCAGACCTTTGGGGCGTCTCCCTGTGCACTGTGGATGGGCAGCGGCACTCTGTGGGCCAC
  ACGAAGATCCCCTTCTGCCTGCAGTCCTGTGTCAAGCCCCTCACTTACGCCATCTCCGTGAGCACCTTAG
  GCACTGACTACGTGCACAAGTTTGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACTCTCCCTCAA
  TGAGGAAGGAATTCCCCATAACCCCATGGTCAATGCTGGTGCCATTGTGGTCAGCTCCCTGATCAAGATG
  GACTGTAACAAAGCAGAGAAGTTCGATTTTGTGTTACAGTATCTGAACAAGATGGCTGGGAACGAATTCA
  TGGGGTTCAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCATCGGCTATTA
  CCTCAAGGAGAAGAAGTGCTTCCCTAAGGGTGTGGACATGATGGCTGCCCTTGATCTCTATTTCCAGCTG
  TGCTCTGTGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTGGCCAATGGCGGCATCTGCC
  CTATCACAGGGGAGAGCGTGCTGAGCGCCGAGGCCGTGCGCAACACCCTCAGCCTCATGCATTCCTGTGG
  CATGTATGACTTCTCGGGCCAGTTTGCCTTCCATGTGGGCCTGCCAGCCAAGTCAGCTGTGTCAGGAGCC
  ATCCTCCTGGTTGTACCCAATGTCATGGGCATGATGTGTCTGTCGCCGCCGTTAGACAAGCTGGGGAACA
  GCCAAAGGGGCATCAACTTCTGCCAGAAGTTGGTGTCTCTCTTTAACTTCCACAACTATGACAACCTGCG
  GCACTGCGCTCGGAAGTTAGACCCACGGAGGGAAGGAGGAGAGGTTCGGAACAAGACCGTGGTGAACCTG
  CTATTTGCTGCATATAGTGGAGATGTCTCGGCCCTTCGAAGGTTTGCATTGTCGGCCATGGATATGGAGC
  AGAAGGACTATGATTCCCGCACAGCCCTACATGTCGCTGCAGCTGAAGGGCACATTGAAGTTGTCAAATT
  TCTGATCGAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTGGACGATGCT
  GTGCAGTTCAACCACCTGGAGGTGGTCAAACTGCTTCAAGATTACCATGACTCCTACTTGCTCTCTGAGA
  CTCAAGCTGAGGCGGCAGCTGAGACCCTGTCCAAAGAGAATCTAGAGAGCATGGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033264
• Insert Size: 1809 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033264.3, NP_001028436.2
• RefSeq Size: 2460 bp
• RefSeq ORF: 1809 bp
• Locus ID: 216456
• UniProt ID: Q571F8
• Cytogenetics: 10 D3
• Gene Summary: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.