Gls2 (NM_001033264) Mouse Tagged ORF Clone

CAT#: MG221638

Gls2 (tGFP-tagged) - Mouse glutaminase 2 (liver mitochondrial) (Gls2) nuclear gene encoding mitochondrial protein, (10ug)

Product Images

Circular map for MG221638

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gls2
• Synonyms: A330074B06Rik; AI195532; GA; GLS; Lga
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221638 representing NM_001033264
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCCATGAGGGCTCTGCAGAACGCGCTGAGCCGCGCGGGCAGCCACGGTCGGCGGGGAGGCTGGG
  GTCATCCGAGCCGGGGCCCGCTTCTGGGCAGGGGCGTCCGGTACTACCTCGGTGAGGCCGCGGCGCAGGG
  CAGGGGGACGCCGCACAGCCACCAGCCGCAGCACTCGGATCATGACGCCTCACACAGCGGCATGCTGCCT
  CGACTTGGTGACCTGCTTTTCTACACTATTGCAGAGGGACAGGAGCGTATCCCTATCCACAAGTTCACCA
  CGGCTCTGAAGGCCACTGGACTGCAGACGTCAGACCCACGGCTCCAGGACTGCATGAGCAAGATGCAGCG
  CATGGTCCAAGAGTCCAGCAGCGGTGGCCTCTTGGATCGAGAGCTCTTCCAAAAGTGTGTGAGCAGCAAC
  ATTGTGCTCCTGACTCAGGCATTCCGAAAGAAGTTTGTCATTCCTGACTTTGAGGAGTTCACGGGCCATG
  TGGATCGCATATTTGAGGATGCCAAAGAGCCCACTGGAGGCAAAGTGGCAGCCTACATCCCGCACCTGGC
  CAAATCAAACCCAGACCTTTGGGGCGTCTCCCTGTGCACTGTGGATGGGCAGCGGCACTCTGTGGGCCAC
  ACGAAGATCCCCTTCTGCCTGCAGTCCTGTGTCAAGCCCCTCACTTACGCCATCTCCGTGAGCACCTTAG
  GCACTGACTACGTGCACAAGTTTGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACTCTCCCTCAA
  TGAGGAAGGAATTCCCCATAACCCCATGGTCAATGCTGGTGCCATTGTGGTCAGCTCCCTGATCAAGATG
  GACTGTAACAAAGCAGAGAAGTTCGATTTTGTGTTACAGTATCTGAACAAGATGGCTGGGAACGAATTCA
  TGGGGTTCAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCATCGGCTATTA
  CCTCAAGGAGAAGAAGTGCTTCCCTAAGGGTGTGGACATGATGGCTGCCCTTGATCTCTATTTCCAGCTG
  TGCTCTGTGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTGGCCAATGGCGGCATCTGCC
  CTATCACAGGGGAGAGCGTGCTGAGCGCCGAGGCCGTGCGCAACACCCTCAGCCTCATGCATTCCTGTGG
  CATGTATGACTTCTCGGGCCAGTTTGCCTTCCATGTGGGCCTGCCAGCCAAGTCAGCTGTGTCAGGAGCC
  ATCCTCCTGGTTGTACCCAATGTCATGGGCATGATGTGTCTGTCGCCACCGTTAGACAAGCTGGGGAACA
  GCCAAAGGGGCATCAACTTCTGCCAGAAGTTGGTGTCTCTCTTTAACTTCCACAACTATGACAACCTGCG
  GCACTGCGCTCGGAAGTTAGACCCACGGAGGGAAGGAGGAGAGGTTCGGAACAAGACCGTGGTGAACCTG
  CTATTTGCTGCATATAGTGGAGATGTCTCGGCCCTTCGAAGGTTTGCATTGTCGGCCATGGATATGGAGC
  AGAAGGACTATGATTCCCGCACAGCCCTACATGTCGCTGCAGCTGAAGGGCACATTGAAGTTGTCAAATT
  TCTGATCGAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTGGACGATGCT
  GTGCAGTTCAACCACCTGGAGGTGGTCAAACTGCTTCAAGATTACCATGACTCCTACTTGCTCTCTGAGT
  CTCAAGCTGAGGCGGCAGCTGAGACCCTGTCCAAAGAGAATCTAGAGAGCATGGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221638 representing NM_001033264
  Red=Cloning site Green=Tags(s)
  
  MRSMRALQNALSRAGSHGRRGGWGHPSRGPLLGRGVRYYLGEAAAQGRGTPHSHQPQHSDHDASHSGMLP
  RLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSN
  IVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKEPTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGH
  TKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKM
  DCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQL
  CSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGA
  ILLVVPNVMGMMCLSPPLDKLGNSQRGINFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNL
  LFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFVKDRWGNIPLDDA
  VQFNHLEVVKLLQDYHDSYLLSESQAEAAAETLSKENLESMV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033264
• ORF Size: 1806 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033264.3, NP_001028436.2
• RefSeq Size: 2460 bp
• RefSeq ORF: 1809 bp
• Locus ID: 216456
• UniProt ID: Q571F8
• Cytogenetics: 10 D3
• Gene Summary: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.[UniProtKB/Swiss-Prot Function]