TdT (DNTT) Human Recombinant Protein

CAT#: TP727648

Recombinant Human DNTT/TDT (N-6His)

Specifications

Product Data

• Species: Human
• Protein Source: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Met1-Ala509
• Tag: N-6His
• Buffer: Supplied as a 0.2 um filtered solution of 0.1M KH2PO4, 200mM KCl, 1mM β-ME, 50% glycerol, pH7.2.
• Note: Recombinant Human DNA nucleotidylexotransferase is produced by our E.coli expression system and the target gene encoding Met1-Ala509 is expressed with a 6His tag at the N-terminus.
• Storage: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 1791
• UniProt ID: P04053
• Synonyms: DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltr; Terminal deoxynucleotidyltransferase; Terminal transferase; DNTT; TDT
• Summary: Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
• Protein Pathways: Hematopoietic cell lineage, Non-homologous end-joining