Escherichia coli Recombinant Protein

CAT#: TP727424

Recombinant E. coli Tryptophan Synthase α Chain/Trp A

Specifications

Product Data

• Species: Escherichia coli
• Protein Source: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Met1-Ser268
• Buffer: Supplied as a 0.2 um filtered solution of PBS, pH 7.4.
• Note: Recombinant E.coli Tryptophan synthase alpha chain is produced by our E.coli expression system and the target gene encoding Met1-Ser268 is expressed.
• Storage: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Stability: 12 months from date of despatch

Reference Data

• Synonyms: Tryptophan synthase alpha chain; trpA
• Summary: Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.