B7-1 (CD80) Human Recombinant Protein

CAT#: TP724008

Human B7-1 Protein, hFc Tag

Product Images

Figure 1. Human B7-1 Protein, hFc Tag on SDS-PAGE under reducing condition.

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  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein (TP724008) can bind Human CTLA4, mFc-His tagged protein (TP723940) in a linear range of 0.048-2.094 ng/ml.

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  Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD28, mFc-His tagged protein (TP723937) can bind Human B7-1, hFc tagged protein (TP724008) in a linear range of 125-4000 ng/ml.

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  Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human CD28 (A) were surface stained with Human B7-1 , hFc tagged protein (TP724008) 1 µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  B7-1（Val35-Asn242）+hFc（Glu99-Ala330）
• Tag: C-Human Fc
• Predicted MW: 50.0 kDa
• Purity: > 95%
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
• Reconstitution: Reconstitute with deionized water
• Preparation: Affinity purification
• Applications: ELISA, Flow Cyt
• Storage: Store the lyophilized protein at -20°C.
  After reconstitution, store the protein at -80°C for 12 months.
  Avoid repeated freezing and thawing.
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 941
• UniProt ID: P33681
• Summary: The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.