SARS-CoV-2 Spike S1 Recombinant Protein with His and Avi tag

CAT#: TP723916

SARS-CoV-2 Spike S1 Recombinant Protein with His and Avi tag

Product Images

Recombinant SARS-COV-2 Spike S1 Protein with His and Avi tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-120 kDa.

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  The purity of SARS-COV-2 Spike S1 Protein with His and Avi tag (Cat.TP723916) was greater than 95% as determined by SEC-HPLC.

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  Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 24.5 nM as determined in a SPR assay (Nicoya OpenSPR).

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  Immobilized Recombinant SARS-CoV-2 Spike S1 at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL.

Specifications

Product Data

• Species: SARS-CoV-2
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  QCVNLTTRTQ LPPAYTNSFT RGVYYPDKVF RSSVLHSTQD LFLPFFSNVT WFHAIHVSGT NGTKRFDNPV LPFNDGVYFA STEKSNIIRG WIFGTTLDSK TQSLLIVNNA TNVVIKVCEF QFCNDPFLGV YYHKNNKSWM ESEFRVYSSA NNCTFEYVSQ PFLMDLEGKQ GNFKNLREFV FKNIDGYFKI YSKHTPINLV RDLPQGFSAL EPLVDLPIGI NITRFQTLLA LHRSYLTPGD SSSGWTAGAA AYYVGYLQPR TFLLKYNENG TITDAVDCAL DPLSETKCTL KSFTVEKGIY QTSNFRVQPT ESIVRFPNIT NLCPFGEVFN ATRFASVYAW NRKRISNCVA DYSVLYNSAS FSTFKCYGVS PTKLNDLCFT NVYADSFVIR GDEVRQIAPG QTGKIADYNY KLPDDFTGCV IAWNSNNLDS KVGGNYNYLY RLFRKSNLKP FERDISTEIY QAGSTPCNGV EGFNCYFPLQ SYGFQPTNGV GYQPYRVVVL SFELLHAPAT VCGPKKSTNL VKNKCVNFNF NGLTGTGVLT ESNKKFLPFQ QFGRDIADTT DAVRDPQTLE ILDITPCSFG GVSVITPGTN TSNQVAVLYQ DVNCTEVPVA IHADQLTPTW RVYSTGSNVF QTRAGCLIGA EHVNNSYECD IPIGAGICAS YQTQTNSPRR
• Tag: C-6xHis/AVI
• Purity: >95% by SDS-PAGE.
• Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Endotoxin: < 1.0 EU/μg of the protein by LAL method.
• Protein Description: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln14-Arg683) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6×His tag and Avi at the C-terminus.
• Storage: Store at -80°C.
• Stability: 3 months

Reference Data

• Locus ID: 43740568