Pfas (NM_001159519) Mouse Recombinant Protein

CAT#: TP517770

Purified recombinant protein of Mouse phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR217770 representing NM_001159519
  Red=Cloning site Green=Tags(s)
  
  MAPVLHFYVRPSGHEGAASGRVFRRLQEKLPTLQSVETELCYNVHWAAETLPWAEEMKKLMWLFGCPLVR
  DDVAQEPWLVPGSNDLLLEVGPRLNFSTPASTNIVSVCQAAGLRAVDRVETTRRYRLSFTDHPTAEMEAI
  SLAALHDRMTEQHYPDPIQSFSPQSIPAPLKGSIDILAEGRPALEKANQELGLALDSWDLDFYTKRFQEL
  QRNPSTVEVFDLAQSNSEHSRHWFFKGQLHVDGKKLAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKK
  VKFLRPEDSTRPSCFQQQQGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGY
  CFGNLHIPDYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREW
  IKPIMFSGGIGSMEAKHVGKKPPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPE
  MEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPEGAIIYTSRFQLGDPTLNALEIWGAEYQ
  ESNALLLRPSDRDFLSRASARERCPACFVGTITGDKRIVLVDDRECLVGKTGQGDAPLTPPTPVDLDLDW
  VLGKMPQKEFFLQRKPPVLQPLALPPELSVRQALNRVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPL
  QTPLADVAVVALSHQECIGAATALGEQPVKSLLDPKAAARLAVSEALTNLVFALVTDLRDVKCSGNWMWA
  AKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVQAPGSLVISAYAVCPDITATVTPD
  LKHPGGKGHLLYVPLSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFHITQGLLKECRLCSGHDVS
  DGGLVTCLLEMAFAGNCGIEVDVPAPGIHALPVLFAEEPGLVLEVQEADVAGVRQRYESAGLRCLELGHT
  GEAGPQAMARISVNKAVVVEEPVGELRALWEETSFQLDLLQAEPRCVIEEKQGLKERTGPSYYLPPTFPV
  ASVPCKPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIRLDTFRGVAFVGGFSYAD
  VLGSAKGWAAAVTFNPQAREELGRFRRRPDTFSLGVCNGCQLLALLGWVGSDPSEEQAEPGQDSQPTQPG
  LLLRHNLSGRFESRWATVRVEPGPALMLRGMEGSVLPVWSAHGEGYMAFSSPELQAKIEAKGLVPLHWAD
  DDGNPTEQYPLNPNGSPGGIAGICSQDGRHLALMPHPERAVRLWQWAWRPSPFDVLPTSPWLQLFINARN
  WTQEDSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 144.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001152991
• Locus ID: 237823
• UniProt ID: Q5SUR0
• Cytogenetics: 11 B3
• Refseq Size: 6369
• Refseq ORF: 4011
• Synonyms: 4432409B16Rik; FGAMS; FGAR-AT; FGARAT; Gm18; PURL; Sofa
• Summary: Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity).[UniProtKB/Swiss-Prot Function]