Nadsyn1 (NM_030221) Mouse Recombinant Protein

CAT#: TP510307

Purified recombinant protein of Mouse NAD synthetase 1 (Nadsyn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR210307 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRKVTVATCALNQWALDFEGNFQRILKSIQIAKGKGARYRLGPELEICGYGCWDHYHESDTLLHSLQVL
  AALLDSPVTQDIICDVGMPIMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWTRSRQTEE
  YVLPRMLQDLTKQKTVPFGDVVLATQDTCVGSEICEELWTPRSPHVDMGLDGVEIITNASGSHHVLRKAH
  TRVDLVTMATSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSIFAQGTQFSLDDVEVLTATLDLEDVRS
  YRAEISSRNLEATRVSPYPRVTVDFALSVSEDLLEPVSEPIEWTYHRPEEEISLGPACWLWDFLRRSKQA
  GFFLPLSGGVDSAASACIVYSMCCLVCDAVKSGNQQVLTDVQNLVDESSYTPQDPRELCGRLLTTCYMAS
  ENSSQETHSRATKLAQLIGSYHINLSIDTAVKAVLGIFSLVTGKLPRFSAHGGSSRENLALQNVQARIRM
  VLAYLFAQLSLWSRGARGSLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCAERFQL
  PVLQTILSAPATAELEPLADGQVSQMDEEDMGMTYAELSIFGRLRKVAKAGPYSMFCKLLNMWRDSYTPT
  QVAEKVKLFFSKYSMNRHKMTTLTPAYHAENYSPDDNRFDLRPFLYNTRWPWQFLCIDNQVLQLERKASQ
  TREEQVLEHLKEPSPIWKQLLPKDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 81.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_084497
• Locus ID: 78914
• UniProt ID: Q711T7
• Cytogenetics: 7 F5
• Refseq Size: 2632
• Refseq ORF: 2178
• Synonyms: 9130012B15Rik
• Summary: Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.[UniProtKB/Swiss-Prot Function]